DIFFERENCE IN THE PERCENTAGE OF PHAGOCYTOSIS OF POLYMORPHONUCLEAR LEUKOCYTES IN PATIENTS WITH DIABETES MELLITUS TYPE I AND II WITH PERIODONTAL DISEASE

Abstract

The purpose of this study was to determine the difference in the percentage of phagocytosis of polymorphonuclear leukocyte in patients with diabetes type I and type II with periodontal disease, recent COVID-19 pandemic that has affected millions of people in the world, it has been shown that patients with diabetes have a greater susceptibility to seriously developing the disease caused by the SARS-CoV-2 virus. Materials and Methods: The study consisted of 20 patients from the Periodontology Postgraduate clinic of the Faculty of Dentistry of the Autonomous University of Tamaulipas, who underwent a sample of 10 ml of heparinized blood was taken and 3 ml of Dextran 3% was added and incubated at 37 ° C for two hours in a serological bath, at the end of this time they were centrifuged at 1500 rpm for 6 minutes, the cells were washed with the GIBCO ™ DMEM culture medium 3 times for further counting and adjusted the population at 2-4 x 10 6 cells / ml. The experimental design consisted in using 200 μl of the cells in GIBCO ™ DMEM culture medium and 300 μl of a pure culture of Staphylococcus aureus was diluted in 0.85% saline solution, both reagents were incubated at 37 ° C for 45 minutes, then the cells were resuspended and it placed 1 drop on a slide, dried, fixed with methanol and stained each smear using the Gram technique to then count 100 cells that phagocytosed and not phagocytosed. Results: Control patients reported an average of 83.20 ± 5.81 phagocytic action, and diabetic patients type I and type II reported an average of 40.70 ± 9.92. Likewise, when analyzing the percentage of non-phagocytosis, the control patients presented an average of 16.80 ± 5.81 and the diabetic patients of 59.30 ± 9.92, which was statistically significant (p = 0.001), by other hand, comparing the percentage of phagocytosis among patients with diabetes type I and type II were not statistically significant, with an average