Evaluation of the effect of tramadol, paracetamol and metamizole on the severity of experimental colitis.

IF 1.3 4区 农林科学 Q2 VETERINARY SCIENCES Laboratory Animals Pub Date : 2023-10-01 Epub Date: 2023-03-24 DOI:10.1177/00236772231163957
Marianne Spalinger, Marlene Schwarzfischer, Anna Niechcial, Kirstin Atrott, Andrea Laimbacher, Paulin Jirkof, Michael Scharl
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Abstract

Application of dextran sodium sulfate (DSS) is often used to induce experimental colitis. Current state of the art is to refrain from the use of analgesics due to their possible interaction with the model. However, the use of analgesics would be beneficial to reduce the overall constraint imposed on the animals. Here, we analyzed the effect of the analgesics Dafalgan (paracetamol), Tramal (tramadol) and Novalgin (metamizole) on DSS-induced colitis. To study the effect of those analgesics in colitis mouse models, acute and chronic colitis was induced in female C57BL6 mice by DSS administration in the drinking water. Analgesics were added to the drinking water on days four to seven (acute colitis) or on days six to nine of each DSS cycle (chronic colitis). Tramadol and paracetamol had minor effects on colitis severity. Tramadol reduced water uptake and activity levels slightly, while mice receiving paracetamol presented with a better overall appearance. Metamizole, however, significantly reduced water uptake, resulting in pronounced weight loss. In conclusion, our experiments show that tramadol and paracetamol are viable options for the use in DSS-induced colitis models. However, paracetamol seems to be slightly more favorable since it promoted the overall wellbeing of the animals upon DSS administration without interfering with typical readouts of colitis severity.

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曲马多、对乙酰氨基酚和安乃近对实验性结肠炎严重程度影响的评估。
右旋糖酐硫酸钠(DSS)的应用通常用于诱导实验性结肠炎。目前的技术状态是避免使用止痛药,因为它们可能与模型相互作用。然而,使用止痛药将有利于减少对动物的总体限制。在这里,我们分析了止痛药Dafalgan(扑热息痛)、Tramal(曲马多)和Novalgin(安乃近)对DSS诱导的结肠炎的影响。为了研究这些镇痛剂在结肠炎小鼠模型中的作用,通过饮用水中DSS给药在雌性C57BL6小鼠中诱导急性和慢性结肠炎。在每个DSS周期的第4天至第7天(急性结肠炎)或第6天至第9天(慢性结肠炎)将镇痛剂添加到饮用水中。曲马多和扑热息痛对结肠炎的严重程度影响较小。曲马多略微降低了小鼠的水分摄取和活动水平,而接受扑热息痛治疗的小鼠整体外观更好。然而,异咪唑显著降低了吸水率,导致体重明显减轻。总之,我们的实验表明,曲马多和扑热息痛是用于DSS诱导的结肠炎模型的可行选择。然而,扑热息痛似乎更有利,因为它在DSS给药后促进了动物的整体健康,而不会干扰结肠炎严重程度的典型读数。
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来源期刊
Laboratory Animals
Laboratory Animals 生物-动物学
CiteScore
4.90
自引率
8.30%
发文量
64
审稿时长
6-12 weeks
期刊介绍: The international journal of laboratory animal science and welfare, Laboratory Animals publishes peer-reviewed original papers and reviews on all aspects of the use of animals in biomedical research. The journal promotes improvements in the welfare or well-being of the animals used, it particularly focuses on research that reduces the number of animals used or which replaces animal models with in vitro alternatives.
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