Preserved T cell but attenuated antibody response in MS patients on fingolimod and ocrelizumab following 2nd and 3rd SARS-CoV-2 mRNA vaccine.

IF 2.5 Q2 CLINICAL NEUROLOGY Multiple Sclerosis Journal - Experimental, Translational and Clinical Pub Date : 2023-04-01 DOI:10.1177/20552173231165196

Sarah Conway, Shrishti Saxena, Clare Baecher-Allan, Rajesh Krishnan, Maria Houtchens, Bonnie Glanz, Taylor J Saraceno, Mariann Polgar-Turcsanyi, Gauruv Bose, Rohit Bakshi, Shamik Bhattacharyya, Kristin Galetta, Tamara Kaplan, Christopher Severson, Tarun Singhal, Lynn Stazzone, Jonathan Zurawski, Anu Paul, Howard L Weiner, Brian C Healy, Tanuja Chitnis

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引用次数: 4

Abstract

Background: There is limited knowledge about T cell responses in patients with multiple sclerosis (MS) after 3 doses of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) mRNA vaccine.

Objectives: Assess the SARS-CoV-2 spike antibody and T cell responses in MS patients and healthy controls (HCs) after 2 doses (2-vax) and 3 doses (3-vax) of SARS-CoV-2 mRNA vaccination.

Methods: We studied seroconversion rates and T cell responses by flow cytometry in HC and MS patients on fingolimod or ocrelizumab.

Results: After 2-vax, 8/33 (24.2%) patients in ocrelizumab group, 5/7 (71.4%) in fingolimod group, and 29/29 (100%) in HC group (P = 5.7 × 10^-11) seroconverted. After 3-vax, 9/22 (40.9%) patients in ocrelizumab group, 19/21 (90.5%) in fingolimod group, and 7/7 (100%) in HC group seroconverted (P = 0.0003). The percentage of SARS-CoV-2 peptide reactive total CD4+ T cells increased in HC and ocrelizumab group but not in fingolimod group after 2-vax and 3-vax (P < 0.0001). The percentage of IFNγ and TNFα producing total CD4+ and CD8+ T cells increased in fingolimod group as compared to HC and ocrelizumab group after 2-vax and 3-vax (P < 0.0001).

Conclusions: MS patients on ocrelizumab and fingolimod had attenuated humoral responses, but preserved cytokine producing T cell responses compared to HCs after SARS-CoV-2 mRNA vaccination.

Clinical trials registration: NCT05060354.

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在第二次和第三次SARS-CoV-2 mRNA疫苗接种后，MS患者使用fingolimod和ocrelizumab保留T细胞，但抗体反应减弱。

背景:对3剂严重急性呼吸综合征冠状病毒2 (SARS-CoV-2) mRNA疫苗后多发性硬化症(MS)患者T细胞反应的了解有限。目的:评估2剂(2-vax)和3剂(3-vax) SARS-CoV-2 mRNA疫苗接种后MS患者和健康对照(hc)的SARS-CoV-2刺突抗体和T细胞反应。方法:采用流式细胞术研究芬戈莫德或奥克雷单抗治疗的HC和MS患者血清转化率和T细胞反应。结果:2-vax后，ocrelizumab组8/33例(24.2%)，fingolimod组5/7例(71.4%)，HC组29/29例(100%)血清转化(P = 5.7 × 10-11)。3-vax后，ocrelizumab组9/22(40.9%)、fingolimod组19/21(90.5%)、HC组7/7(100%)的患者血清转化(P = 0.0003)。2-vax和3-vax后，HC组和奥克雷珠单抗组的SARS-CoV-2肽反应性总CD4+ T细胞百分比增加，而芬戈莫德组则没有(P P)结论:与接种SARS-CoV-2 mRNA疫苗后的HC相比，奥克雷珠单抗和芬戈莫德组的MS患者的体液反应减弱，但细胞因子产生的T细胞反应保留。临床试验注册:NCT05060354。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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