Polymer Texture Influences Cell Responses in Osteogenic Microparticles.

IF 2.3 4区医学 Q3 BIOPHYSICS Cellular and molecular bioengineering Pub Date : 2022-10-01 DOI:10.1007/s12195-022-00729-9

Catherine E Miles, Stephanie L Fung, N Sanjeeva Murthy, Adam J Gormley

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Abstract

Introduction: Polymer materials used in medical devices and treatments invariably encounter cellular networks. For the device to succeed in tissue engineering applications, the polymer must promote cellular interactions through adhesion and proliferation. To predict how a polymer will behave in vitro, these material-cell interactions need to be well understood.

Methods: To study polymer structure-property relationships, microparticles of four chemically distinct tyrosol-derived poly(ester-arylate) polymers and a commercially available poly(lactic acid-co-glycolic acid) (PLGA) copolymer were prepared and their interactions with cells investigated. Cell loading concentration was optimized and cell adhesion and proliferation evaluated. Particles were also tested for their ability to adsorb bone morphogenetic protein-2 (BMP-2) and differentiate a myoblast cell line towards an osteoblast lineage through BMP-2 loading and release.

Results: While cell adhesion was observed on all particles after 24 h of incubation, the highest degree of cell adhesion occurred on polymers with smaller crystallites. At longer incubation times, cells proliferated on all particle formulations, regardless of the differences in polymer properties. High BMP-2 loading was achieved for all particle formulations and all formulations showed a burst release. Even with the burst release, cells cultured on all formulations showed an upregulation in alkaline phosphatase (ALP) activity, a measure of osteoblast differentiation.

Conclusions: As with cell adhesion, the polymer with the smaller crystallite showed the most ALP activity. We suggest that smaller crystallites serve as a proxy for topographical roughness to elicit the observed responses from cells. Furthermore, we have drawn a correlation between the polymer crystallite with the hydration potential using surface analysis techniques.

Graphical abstract:

Supplementary information: The online version contains supplementary material available at 10.1007/s12195-022-00729-9.

Abstract Image

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聚合物结构影响成骨微粒的细胞反应。

简介:用于医疗设备和治疗的聚合物材料总是会遇到蜂窝网络。为了使该装置在组织工程应用中取得成功，聚合物必须通过粘附和增殖来促进细胞相互作用。为了预测聚合物在体外的表现，需要很好地理解这些材料-细胞的相互作用。方法:为了研究聚合物的结构-性能关系，制备了四种化学性质不同的tyrosol衍生的聚(酯-芳酸酯)聚合物和一种市售的聚乳酸-共乙醇酸(PLGA)共聚物的微粒，并研究了它们与细胞的相互作用。优化细胞加载浓度，评价细胞的粘附和增殖能力。我们还测试了颗粒吸附骨形态发生蛋白-2 (BMP-2)的能力，并通过BMP-2的加载和释放将成肌细胞分化为成骨细胞谱系。结果:孵育24 h后，所有颗粒均有细胞粘附，其中晶体较小的聚合物细胞粘附程度最高。在较长的孵育时间内，细胞在所有颗粒配方上增殖，而不管聚合物性质的差异。所有颗粒配方均获得了高BMP-2负载，并且所有配方均显示出爆发释放。即使有爆发释放，在所有配方中培养的细胞都显示碱性磷酸酶(ALP)活性上调，这是成骨细胞分化的一种衡量标准。结论:与细胞粘附一样，晶体越小的聚合物ALP活性越高。我们认为，较小的晶体可以作为地形粗糙度的代表，从而引起细胞的观察反应。此外，我们还利用表面分析技术绘制了聚合物晶体与水化电位之间的关系。图片摘要:补充资料:在线版本包含补充资料，网址为10.1007/s12195-022-00729-9。

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来源期刊

Cellular and molecular bioengineering 生物-生物物理

CiteScore

5.60

自引率

3.60%

发文量

审稿时长

>12 weeks

期刊介绍： The field of cellular and molecular bioengineering seeks to understand, so that we may ultimately control, the mechanical, chemical, and electrical processes of the cell. A key challenge in improving human health is to understand how cellular behavior arises from molecular-level interactions. CMBE, an official journal of the Biomedical Engineering Society, publishes original research and review papers in the following seven general areas: Molecular: DNA-protein/RNA-protein interactions, protein folding and function, protein-protein and receptor-ligand interactions, lipids, polysaccharides, molecular motors, and the biophysics of macromolecules that function as therapeutics or engineered matrices, for example. Cellular: Studies of how cells sense physicochemical events surrounding and within cells, and how cells transduce these events into biological responses. Specific cell processes of interest include cell growth, differentiation, migration, signal transduction, protein secretion and transport, gene expression and regulation, and cell-matrix interactions. Mechanobiology: The mechanical properties of cells and biomolecules, cellular/molecular force generation and adhesion, the response of cells to their mechanical microenvironment, and mechanotransduction in response to various physical forces such as fluid shear stress. Nanomedicine: The engineering of nanoparticles for advanced drug delivery and molecular imaging applications, with particular focus on the interaction of such particles with living cells. Also, the application of nanostructured materials to control the behavior of cells and biomolecules.