紫舌假单胞菌YJR13和腐臭假单胞菌YJR92抗辣椒疫霉菌活性及辣椒根定殖

IF 1.8 3区 农林科学 Q2 PLANT SCIENCES Plant Pathology Journal Pub Date : 2023-02-01 DOI:10.5423/PPJ.OA.01.2023.0001
Elena Volynchikova, Ki Deok Kim
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引用次数: 1

摘要

此前,通过序筛选,plecogssidida YJR13和Pseudomonas putida YJR92被证明能有效控制辣椒疫霉引起的疫病。在本研究中,我们进一步研究了这些菌株对辣椒粉菌丝生长、游动孢子萌发和胚管伸长的抑制活性。我们还研究了菌株在方形培养皿中的根定植能力,包括细胞运动(游泳和群体运动)和生物膜形成。两株菌株在液体和固体V8果汁培养基和M9培养基中,与芽孢杆菌(Bacillus vallismortis) EXTN-1(阳性生防菌株)、水鞘单胞菌(Sphingomonas aquatilis KU408(阴性生防菌株)和MgSO4溶液(未处理对照)相比,均显著抑制菌丝生长、虫孢子萌发和芽管伸长。在稀释的(营养缺乏的)V8果汁肉汤中,所测试的菌株数量保持在>108个细胞/ml,同时提供菌丝抑制活性。此外,这些菌株以106个细胞/ml的浓度在辣椒根部定植7天。菌株的根定植受到强大的游动和群体活动、生物膜形成以及对辣椒根系渗出成分(氨基酸、有机酸和糖)的趋化活性的支持。综上所述,菌株YJR13和YJR92可以通过直接抑制卵菌对辣椒菌丝生长、虫孢子萌发和胚管伸长的抑制作用,有效抑制辣椒疫霉病。细菌在辣椒根中的定植可能通过细胞运动、生物膜的形成以及对根分泌物的趋化性来介导。
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Anti-Oomycete Activity and Pepper Root Colonization of Pseudomonas plecoglossicida YJR13 and Pseudomonas putida YJR92 against Phytophthora capsici.

Previously, Pseudomonas plecoglossicida YJR13 and Pseudomonas putida YJR92 from a sequential screening procedure were proven to effectively control Phytophthora blight caused by Phytophthora capsici. In this study, we further investigated the anti-oomycete activities of these strains against mycelial growth, zoospore germination, and germ tube elongation of P. capsici. We also investigated root colonization ability of the bacterial strains in square dishes, including cell motility (swimming and swarming motilities) and biofilm formation. Both strains significantly inhibited mycelial growth in liquid and solid V8 juice media and M9 minimal media, zoospore germination, and germ tube elongation compared with Bacillus vallismortis EXTN-1 (positive biocontrol strain), Sphingomonas aquatilis KU408 (negative biocontrol strain), and MgSO4 solution (untreated control). In diluted (nutrient-deficient) V8 juice broth, the tested strain populations were maintained at >108 cells/ml, simultaneously providing mycelial inhibitory activity. Additionally, these strains colonized pepper roots at a 106 cells/ml concentration for 7 days. The root colonization of the strains was supported by strong swimming and swarming activities, biofilm formation, and chemotactic activity towards exudate components (amino acids, organic acids, and sugars) of pepper roots. Collectively, these results suggest that strains YJR13 and YJR92 can effectively suppress Phytophthora blight of pepper through direct anti-oomycete activities against mycelial growth, zoospore germination and germ tube elongation. Bacterial colonization of pepper roots may be mediated by cell motility and biofilm formation together with chemotaxis to root exudates.

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来源期刊
Plant Pathology Journal
Plant Pathology Journal 生物-植物科学
CiteScore
4.90
自引率
4.30%
发文量
71
审稿时长
12 months
期刊介绍: Information not localized
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