mec基因破坏对胡萝卜乳杆菌甲硫氨酸合成的影响。胡萝卜素Pcc21和减少软腐病。

IF 1.8 3区 农林科学 Q2 PLANT SCIENCES Plant Pathology Journal Pub Date : 2023-02-01 DOI:10.5423/PPJ.OA.09.2022.0135
Seonmi Yu, Jihee Kang, Eui-Hwan Chung, Yunho Lee
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引用次数: 1

摘要

植物致病性胸腺杆菌在世界上许多重要的经济作物中引起严重的软腐病/黑腿病。乳杆菌利用植物细胞壁降解酶(PCWDEs)作为其致病性的主要毒力决定因素。在这项研究中,我们筛选了一个随机突变,M29是编码半胱氨酸β-裂解酶的metC基因的转座子插入突变,该酶在蛋氨酸生物合成的倒数第二步催化半胱氨酸转化为同型半胱氨酸。M29成为蛋氨酸营养不良者,在蛋氨酸限制条件下导致生长缺陷。用外源蛋氨酸或同型半胱氨酸而不是半胱硫氨酸恢复受损的生长。该突变体在白菜和马铃薯块茎中表现出较弱的软腐病症状,保持了PCWDEs的活性和游泳运动。该突变体不能在白菜和马铃薯块茎中增殖。补充菌株或100µM蛋氨酸可部分恢复原毒力,而极高浓度(1 mM)可完全恢复原毒力。我们的转录组学分析显示,参与蛋氨酸生物合成或转运的基因在突变体中下调。我们的研究结果表明,MetC是蛋氨酸生物合成和转运的重要载体,并通过Pcc21在植物宿主中的增殖影响其毒力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Disruption of the metC Gene Affects Methionine Biosynthesis in Pectobacterium carotovorum subsp. carotovorum Pcc21 and Reduces Soft-Rot Disease.

Plant pathogenic Pectobacterium species cause severe soft rot/blackleg diseases in many economically important crops worldwide. Pectobacterium utilizes plant cell wall degrading enzymes (PCWDEs) as the main virulence determinants for its pathogenicity. In this study, we screened a random mutant, M29 is a transposon insertion mutation in the metC gene encoding cystathionine β-lyase that catalyzes cystathionine to homocysteine at the penultimate step in methionine biosynthesis. M29 became a methionine auxotroph and resulted in growth defects in methionine-limited conditions. Impaired growth was restored with exogenous methionine or homocysteine rather than cystathionine. The mutant exhibited reduced soft rot symptoms in Chinese cabbages and potato tubers, maintaining activities of PCWDEs and swimming motility. The mutant was unable to proliferate in both Chinese cabbages and potato tubers. The reduced virulence was partially restored by a complemented strain or 100 µM of methionine, whereas it was fully restored by the extremely high concentration (1 mM). Our transcriptomic analysis showed that genes involved in methionine biosynthesis or transporter were downregulated in the mutant. Our results demonstrate that MetC is important for methionine biosynthesis and transporter and influences its virulence through Pcc21 multiplication in plant hosts.

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来源期刊
Plant Pathology Journal
Plant Pathology Journal 生物-植物科学
CiteScore
4.90
自引率
4.30%
发文量
71
审稿时长
12 months
期刊介绍: Information not localized
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