氨基酸苯二胺应用对塔拉斯芽(学名Colocasia esculenta L)的重复作用。网络文化

Muahammad Sofa, Karmanah Karmanah, Yunus Arifien
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摘要

在短时间内产生种子的一种方法是通过离体培养的无性繁殖。本研究旨在研究施用一定浓度的苄基氨基嘌呤(BAP)对芋头幼芽增殖的影响。该研究于2020年8月至2021年2月在茂物市粮食和农业安全局实验室进行。试验以BAP浓度为单因素,采用完全随机设计。4个处理水平和1个对照的BAP浓度。生长调节剂(ZPT)处理为P0(对照)、Pl (0.5 mg/L BAP + 0.2 mg/L NAA)、P2 (1 mg/L BAP + 0.2 mg/L NAA)、P3 (1.5 mg/L BAP + 0.2 mg/L NAA)、P4 (2 mg/L BAP + 0.2 mg/L NAA)。处理水平和对照水平各设4个重复,共设20个实验单元。观察参数包括茎高、芽数、叶数和根数。本研究表明,在Murashige和Skoog基础培养基上施用BAP对芋头植株增殖的芽数和根数均有影响。P2处理对弯芋植株的体外增殖刺激效果最好。P1处理能显著提高植株的茎高、芽数和叶片数量,而P1处理能显著提高根系数量。
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PENGARUH APLIKASI BENZIL AMINO PURIN TERHADAP MULTIPLIKASI TUNAS TALAS (Colocasia esculenta L.) SECARA KULTUR JARINGAN
One way to produce seeds in a short time is through vegetative propagation by in vitro culture. This study aims to determine the effect of the application of a certain concentration of Benzyl Amino Purine (BAP) on the multiplication of taro shoots ( Colocasia esculenta L.). The research conducted at the Laboratory of the Food and Agriculture Security Service of Bogor City, in August 2020 – February 2021. The test was carried out with BAP concentration as a single factor in completely randomized design. Concentration of BAP with 4 treatment levels and one control. The treatments of growth regulators (ZPT) were P0 (Control), Pl (0.5 mg/L BAP + 0.2 mg/L NAA), P2 (1 mg/L BAP + 0.2 mg/L NAA), P3 (1.5 mg/L BAP + 0.2 mg/L NAA), P4 (2 mg/L BAP + 0.2 mg/L NAA). The replications number for each treatment and control level was 4, so that the total experimental units were 20. Observation parameters included height of shoot, number of shoots, leaves, and roots. This research showed that the application of BAP on Murashige and Skoog basic media affected the number of shoots also the roots in the multiplication of taro plants. The P2 was the best treatment in stimulating the multiplication of bentul taro plants in vitro. This treatment could induce shoot height, number of shoots, and leaves while the P1 treatment increased the number of roots.
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