Anti-wrinkle and anti-inflammation effects of Camellia sinensis seed extracts

The aim of this study was to investigate toxicity, elastase inhibitory activity, collagenase inhibitory activity, procollagen biosynthesis, hyaluronidase inhibitory activity and nitric oxide production inhibitory activity for improving anti-wrinkle and anti-inflammation using different extracts obtained from Camellia sinensis seeds. All extracts showed no cytotoxicity to Hs68 cell within 500 μg/mL. Among various extracts, elastase and collagenase inhibitory activities showed the highest inhibitory activity of 58.6% and 69.1% in 500 μg/mL of ethanol extract, respectivey. Procollagen biosynthesis showed high activity at 158.8% and 157.6% at 500 μg/mL of ethylacetate extract and ethanol extract, respectively. Hyaluronidase inhibitory activity showed the highest inhibitory activity at 31.2% in 500 μg/mL of ethylacetate extract, and nitric oxide production inhibitory activity showed high inhibitory activity at 40.4% in 500μg/mL of ethylacetate extract. The hyaluronidase and nitric oxide production was suppressed in ethylacetate extract, and it is considered that it can be applied to the treatment of inflammatory diseases. Taken together, the ethanol and ethylacetate extracts of C. sinensis seeds used in this study can be expected for skin protection and anti-inflammatory effects.