田菁对小菜蛾综合防治的杀虫效果评价

Suripto Suripto, Lalu Japa, Hilman Ahyadi, Rachmawati Noviana Rahayu
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引用次数: 0

摘要

关于使用当地天然材料作为杀虫剂来源的研究,除其他外,旨在减少农民对使用合成化学品的依赖。本研究旨在从田菁植物中获得防治白菜毛虫最具选择性的杀虫活性成分,选择对环境有安全影响的靶点作用方式,并测定施药前材料在贮存期间的杀虫稳定性和施药后材料在水中的稳定性。采用正己烷、DCM、乙醇、水等溶剂,分阶段提取山楂叶干粉。对各提取部位进行了对小菜蛾幼虫和半月牙蛾的致死毒性试验。对每组死亡数据进行概率分析,得到LC50(s),以确定其杀虫选择性。利用山楂叶提取水进行生物测定,采用probit分析方法测定其对小菜蛾的抑卵、杀卵和抗摄食性能。根据提取前山楂的贮存时间、溶出前山楂的贮存时间和施用过程中山楂在水中的稳定性变化,对山楂的杀虫稳定性进行了研究。数据采用方差分析处理。结果表明,山楂叶乙醇提取物对小菜蛾的杀虫活性具有很强的选择性,即对小菜蛾有很强的毒性,而对半月桂蛾的毒性很小。山楂叶提取物对小菜蛾也有显著的防产卵、抑制卵孵化和取食活性的作用。山楂提取物贮存时间不超过7天,山楂提取物贮存时间不超过3个月,山楂的杀虫力没有明显下降。然而,在应用24小时后,提取物溶液的毒性急剧下降
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Evaluation of Insecticidal Performances of Jayanti Plant (Sesbania sesban) for Integrated Control of Cabbage Caterpillar (Plutella xylostella)
The study on the use of local natural materials as a source of insecticides is intended, among other things, to reduce farmers' dependency on using synthetic chemicals. This research was conducted to obtain the most selective insecticidal active ingredient from Sesbania sesban plant for controlling cabbage caterpillars, choose a target mode of action that has a safe impact on the environment, and determine insecticidal stability during storage of the material prior to application and its stability in water after application. The dried powder of S. sesban leaves was extracted in stages using a series of solvents, namely hexane, DCM, ethanol and water, respectively. Each of the extract fractions produced was tested for its lethal toxicity against Plutella xylostella larvae and Diadegma semiclausum imago. Each of mortality data were processed by probit analysis to produce LC50(s) to determine their insecticidal selectivity. Subsequent bioassays were carried out using extract-water of S. sesban leaves and the data were processed by probit analysis to determine their anti-ovipositor, ovicidal powers and anti-feedant properties against P. xylostella. The insecticidal stability of S. sesban was also studied according to variations in the storage time of the simplicia before extraction, the storage time of the extract before dissolution, and its stability in water during application. The data was processed using ANOVA. The results show that the insecticidal activity of the ethanol extract fraction of S. sesban leaves for controlling cabbage caterpillars was very selective, namely very toxic to P. xylostella but very less toxic to D. semiclausum. Against P. xylostella, S. sesban leaf extract has also been proven to significantly prevent oviposition, inhibit egg hatching and feeding activity. The insecticidal power of S. sesban did not decrease significantly during storage of the simplicia for less than three months and during storage of the extract for less than seven days. However, the toxicity of the extract solution decreased drastically after 24 hours of application
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