尼日利亚Gusau镇疟疾病媒的分子鉴定

K. Abdullahi, S. I. Moyi, M.T. Abdulazeez
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摘要

目前已知的按蚊约有460种,其中100多种可以传播人类疟疾,但只有30-40种通常传播导致疟疾的疟原虫属寄生虫。冈比亚按蚊是非洲最危险的疟疾寄生虫——恶性疟原虫的主要传播媒介。冈比亚按蚊是一个由8个形态上难以区分的物种组成的复合体,复合体中的每一个成员都具有独特的生物学、生态学和行为,应该进行研究和区分。研究目的是利用分子生物学技术鉴定尼日利亚Gusau镇疟疾病媒的种类,以确定准确的疟疾病媒。了解特定环境中的确切疟疾病媒,有助于制定精心设计的量身定制的病媒控制措施。采用标准采集方法,在姑索镇5个区选定地区采集室内和室外蚊虫样本,为期12个月。采用聚合酶链反应(PCR)技术对样本进行鉴定,结果显示所有采集点均为冈比亚按蚊。因此,建议在蚊虫控制规划中采用综合疟疾病媒控制,因为冈比亚按蚊是顽强的,由于出现杀虫剂抗性及其与人类宿主的密切联系,可能难以控制和/或根除。
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Molecular Identification of Malaria Vector in Gusau Township, Nigeria
There about 460 Anopheles species recognized, over 100 can transmit human malaria but only 30–40 commonly transmit parasites of the genus Plasmodium, which cause malaria. Anopheles gambiae is the principal vector of the most dangerous malaria parasite species in Africa, which is Plasmodium falciparum. Anopheles gambiae are a complex consisting of eight morphologically indistinguishable species and each of the members of the complex having unique biology, ecology and behaviour and should be studied and differentiated. The research objective was to identify the species of malaria vector in Gusau township Nigeria using molecular biological technique to know the exact malaria vector. Knowledge of the exact malaria vector in a given environment enables formulating a carefully designed tailor-made vector control measure. Indoor and outdoor mosquito samples were collected from selected areas in five wards of Gusau township using standard collection methods, for a period of twelve months. The samples were identified using polymerase chain reaction (PCR) technique and the result showed Anopheles gambiae in all sites of sample collection. It is therefore recommended that integrated malaria vector control should be adopted in the mosquito control programme because Anopheles gambiae is rugged and can be difficult to control and/or eradicate because of the emerging insecticide resistance and its close association with human host.
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