羽毛水解液的生产优化及富氮肥在水稻生产中的应用

Sumita Sahoo, Biswajit Rath, Keshab C. Mondal, Suman Kumar Halder, Arpita Mandal
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摘要

目前的农业部门主要依靠合成肥料来获得更好的作物。羽毛是蛋白质和氮的丰富来源。在禽窝底培养基中,用角蛋白分解细菌魏德曼尼芽孢杆菌SAB10降解。采用固体发酵工艺生产羽毛水解产物,并通过OVAT (One Variable At A Time)系统对发酵条件进行优化。在pH值为10的条件下,在48小时内将1.25%w/v的羽毛在1%w/v的鸡粪中完全降解。发酵后的游离羽毛水解物以不同的浓度和不同的方式用于水稻植株。在盆栽条件下,固体发酵产生的液态羽毛水解液中含有大量的蛋白质(3.12mg/ml)和氨基酸(792µg/ml),促进了水稻植株的生长。据报道,D组植物叶片的总叶绿素(5.25mg/g干wt)和IAA(17.23µg/ml)含量较高。喷施羽毛水解液(300µl/ml)后,水稻中酚类(1.71倍)和类黄酮(1.52倍)含量显著提高。我们调查的新颖之处在于,我们在这里使用了两种废弃的产品,并将它们转化为有价值的产品。
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Production Optimization of Feather Hydrolysate and Use as a Promising Nitogen-Rich Fertilizer for Rice (Oryza Sativa) Production
Present agriculture sector mostly depend on synthetic fertilizer for better crop. Feather is a rich source of protein and nitrogen. It was degraded by Keratinolytic bacteria Bacillus wiedmanni SAB10 in poultry litter sole media. Feather hydrolysate was produce from solid state fermentation process and fermentation condition was optimized through OVAT (One Variable At A Time) system. In this process feather (1.25%w/v) was fully degraded in poultry litter(1%w/v) with in 48 hrs at pH 10.After the fermentation cell free feather hydrolysate use in rice plant in different concentration and different mode. Liquid feather hydrolysate produced from solid state fermentation contain important amount of protein (3.12mg/ml) and amino acid(792µg/ml) that enhances the rice plant growth in pot trial condition. After application Group D Plants leaves have been reported to have higher levels of total chlorophyll (5.25mg/g of dry wt), IAA (17.23µg/ml)..Carbohydrate contain of rice has increased 1.6 fold than control Following the spraying of feather hydrolysate (300 µl/ml), the phenolic (1.71 fold) and flavonoid (1.52 fold) contents significantly increased.. The novelty of our investigation is we use here two wasted products and convert them a valuable product.
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