环境因素对椭圆酵母BSU-XR1生产纳米银的影响

Khudaverdi G. Ganbarov, Mirmusa M. Jafarov, Sanam İ. Huseynova, Muhammad Shoaib, Kamala S. Alkishiyeva
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摘要

摘要:本文研究了不同环境因素对椭圆酵母BSU-XR1合成纳米银的影响。在30℃的肉汤培养基中培养酵母形成纳米银颗粒。用过滤器从肉汤中分离生物质。生物质用0.1L蒸馏水(DW)洗涤3次。将10克湿生物质倒入装有90毫升无菌DW的烧杯中。将1 ml的10-3摩尔硝酸银溶液倒入其中,与硝酸银盐混合,在黑暗环境中孵育。结果表明,在10克湿生物质中,培养第21天为制备纳米银颗粒的最佳条件。对于所研究的酵母菌株的培养,银纳米颗粒的合成在25-30°C的范围内进行。培养基初始pH为7.0。以硝酸银为盐,浓度分别为0.5 mM和1.0 mM,各实验的最佳培养条件均为黑暗环境。
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Impact of Environmental Factors on the Production of Silver Nanoparticles by Saccharomyces Ellipsoideus BSU-XR1
ABSTRACT: In our presented work we have studied the effect of various environmental factors on the synthesis of silver nanoparticles by Saccharomyces ellipsoideus BSU-XR1. Silver nanoparticles were formed by growing the yeast in broth medium at 30oC. Filters were used to seperate biomass from broth. Biomass was washed 3 times with 0.1L of distilled water (DW). 10 grams of wet biomass was poured to beaker containing 90 ml of sterile DW. 1 ml of 10-3 molar solution of Silver nitrate was poured to it, and this mixture with silver nitrate salt was incubated in a dark environment. It was determined that, the optimal condition for the production of silver nanoparticles was being on the 21st day of incubation, in 10 grams of wet biomass. For the cultivation of the studied yeast strain, the synthesis of silver nanoparticles took place in the range of 25-30°C. Tthe initial pH of the medium was 7.0. and the concentration of silver nitrate was used as salt at conntration of 0.5 mM and 1.0 mM. The optimal conditions of incubation were in dark environment in all variotions of experiments.
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