荞麦茎皮水提物和甲醇提物抗炎、抗伤及抗氧化活性研究Milne-Redh。(芸香料)

Peter Maloba, Elias Mandela, Sammy Kimoloi, Jared Onyancha
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Total phenolic and flavonoid contents were determined by Folin-Ciocalteu and aluminium chloride calorimetric methods, respectively. Anti-inflammatory and anti-nociceptive activities of increasing water and methanol extracts doses (2-250mg/Kg) were tested using carrageenan-induced paw edema and acetic acid-induced writhing animal models, respectively in comparison with dexamethasone and aspirin as standard. Antioxidant activities of plant extracts were assayed by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) method, with ascorbic acid as the standard. Data analysis was conducted by two-way ANOVA with appropriate post hoc tests. Statistical significance level was set at p ≤ 0.05. Results: Phenols, flavonoids, alkaloids, saponins, tannins, terpenoids and coumarin were detected in the extracts. However, anthocyanins and anthraquinones were absent in both water and methanolic extracts. 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引用次数: 0

摘要

背景:Fagaropsis hildebrandtii在非洲传统医学中被广泛用于治疗各种疾病,特别是与炎症、疼痛和氧化应激有关的疾病。然而,关于黄芪不同部位的抗炎、抗伤害和抗氧化作用的实验数据仍然有限。目的:测定产自肯尼亚Makueni县的hildebrandtii茎皮提取物的植物化学成分、抗炎、抗伤和抗氧化活性。材料与方法:采用水浸法和甲醇浸渍法提取大黄茎皮。然后使用标准的定性方法来确定提取物中各种植物化学物质的存在与否。用福林- ciocalteu法和氯化铝量热法分别测定总酚和类黄酮含量。采用角叉菜胶致足跖水肿动物模型和醋酸致足跖扭体动物模型,分别以地塞米松和阿司匹林为对照,观察增加水提取物和甲醇提取物剂量(2 ~ 250mg/Kg)的抗炎和抗伤害活性。以抗坏血酸为标准,采用2,2-二苯基-1-苦酰肼(DPPH)法测定植物提取物的抗氧化活性。数据分析采用双向方差分析和适当的事后检验。p≤0.05为差异有统计学意义。结果:提取液中检出酚类、黄酮类、生物碱类、皂苷类、单宁类、萜类、香豆素类。而水和甲醇提取物均不含花青素和蒽醌类。值得注意的是,甲醇提取物的总酚含量高于水提取物(34.590±2.490 mgGAE/g vs. 7.893±1.619 mgGAE/g);术中,0.05)。甲醇提取物总黄酮含量也高于水提取物(132.18±0.26 mgCE/g vs. 42.68±0.93mgCE/g);术中,0.05)。水提物和甲醇提物对自由基的清除活性IC50分别为0.98µg/ml和0.987µg/ml,显著低于抗坏血酸的清除活性5.674µg/ml,具有较高的抗氧化能力。与10 mg/Kg地塞米松标准品相比,鹿角菜提取物对卡拉胶诱导的后足水肿的抑制率在5小时内无显著差异(p>0.05)。水提物和甲醇提物对小鼠醋酸致扭体的抑制作用呈剂量依赖性,在250 mg/Kg时最大,分别为53.1±6.1%和80.24±12.59%。值得注意的是,50 mg/kg和250 mg/kg的牛蒡醇提物对醋酸致扭体的抑制百分比与150mg /kg的阿司匹林标准物相似(77.75±19.64% vs. 80.24±12.59% vs. 84.99±3.26%,p>0.05,双向方差分析)。结论:黄芪具有抗炎、抗炎、抗氧化作用。这些发现证实了在肯尼亚少数民族中使用F. hildebrandtii粗提取物来治疗与炎症、疼痛和氧化应激相关的疾病。
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Antiinflammatory, Antinociceptive and Antioxidant activities of aqueous and methanolic stem bark extracts of Fagaropsis hildebrandtii (Engl.) Milne-Redh. (Rutaceae)
Background: Fagaropsis hildebrandtii has been widely used in African traditional medicine to treat various illnesses, particularly those associated with inflammation, pain and oxidative stress. However, experimental data on the anti-inflammatory, anti-nociceptive and antioxidant of the different parts of F. hildebrandtii is still limited. Objectives: The objectives of this study were to determine the phytochemical composition, anti-inflammatory, antinociceptive and antioxidant activities of stem bark extract of F. hildebrandtii from Makueni County, Kenya. Materials and Methods: The stem bark of F. hildebrandtii was extracted by maceration using water and methanol. Standard qualitative methods were then used to determine the presence or absence of various phytochemicals in the extracts. Total phenolic and flavonoid contents were determined by Folin-Ciocalteu and aluminium chloride calorimetric methods, respectively. Anti-inflammatory and anti-nociceptive activities of increasing water and methanol extracts doses (2-250mg/Kg) were tested using carrageenan-induced paw edema and acetic acid-induced writhing animal models, respectively in comparison with dexamethasone and aspirin as standard. Antioxidant activities of plant extracts were assayed by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) method, with ascorbic acid as the standard. Data analysis was conducted by two-way ANOVA with appropriate post hoc tests. Statistical significance level was set at p ≤ 0.05. Results: Phenols, flavonoids, alkaloids, saponins, tannins, terpenoids and coumarin were detected in the extracts. However, anthocyanins and anthraquinones were absent in both water and methanolic extracts. Notably, methanol extract had a high total phenolic level compared to water extract (34.590 ± 2.490 mgGAE/g vs. 7.893 ± 1.619 mgGAE/g; p<0.05). Total flavonoid content of F. hildebrandtii methanol extract was also higher in comparison to water extract (132.18 ± 0.26 mgCE/g vs. 42.68 ± 0.93mgCE/g; p<0.05). F. hildebrandtii water and methanol extracts had a radical scavenging activity IC50 of 0.98 µg/ml and 0.987 µg/ml respectively, which was significantly lower than 5.674 µg/ml for ascorbic acid, indicating a higher antioxidant potency. The percentage inhibition of carrageenan-induced hind paw edema by F. hildebrandtii extracts was not significantly different in comparison with 10 mg/Kg of dexamethsone standard over a 5-hour test period (p>0.05). F. hildebrandtii water and methanol extracts exhibited a dose-dependent inhibition of acetic acid-induced writhing in mice, reaching a maximum of 53.1 ± 6.1% and 80.24 ± 12.59%, respectively, at 250 mg/Kg. Notably, the percentage inhibition of acetic acid-induced writhing by 50 mg/kg and 250 mg/kg of F. hildebrandtii methanolic extract was similar to that of aspirin standard at 150 mg/kg (77.75 ± 19.64% vs. 80.24 ± 12.59% vs. 84.99 ± 3.26%, p>0.05, two-way ANOVA). Conclusion: F. hildebrandtii has anti-inflammatory, antinociceptive and antioxidant activities. These findings validate the use of F. hildebrandtii crude extracts among Kenyan ethnic groups in management of diseases associated with inflammation, pain, and oxidative stress.
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