酶解单峰骆驼乳蛋白的双重功能特性及抗氧化和抗菌活性的提高

Dina Awad, Osama Ahmed, Samar Baloza, Maha Mamdouh
{"title":"酶解单峰骆驼乳蛋白的双重功能特性及抗氧化和抗菌活性的提高","authors":"Dina Awad, Osama Ahmed, Samar Baloza, Maha Mamdouh","doi":"10.21608/bvmj.2023.228158.1700","DOIUrl":null,"url":null,"abstract":"Keywords Dromedary milk has a proven outstanding history of being a nutrient-rich food. Camel milk possesses both medicinal and technological advantages, including antioxidant, antibacterial, and antiparasitic activities. The present investigation is proposed to assess the antibacterial ability and free radical scavenging capacity against Gram-negative bacteria of various enzyme-digested skimmed camel’s milk (SCM) using papain, pepsin, and trypsin at four hydrolysis times at one-hour intervals. An antibacterial growth assay was conducted to detect the antibacterial capacity of the generated various enzymatic camel milk hydrolysates. The results showed that the antibacterial activity of unhydrolyzed intact SCM displayed mild effect against Escherichia coli and no effect against Salmonella enteritidis . Pepsin-treated SCM hydrolysates after 3h hydrolysis significantly enhanced SCM antimicrobial activity against E. coli , and after 2h, hydrolysis produces SCM hydrolysates against Salmonella enteritidis. Pepsin and trypsin SCM-treated hydrolysates after 2h hydrolysis could completely abolish the survival growth rate of Salmonella enteritidis. Additionally, hydrolysates treated with papain SCM after 3 and 4 hours of hydrolysis showed a 100% ability to kill Salmonella enteritidis . Both DPPH method and ABTS assays were employed to evaluate antioxidants. Our findings demonstrated that all SCM hydrolysates gradually acquired strong antioxidant capacity after four hours of hydrolysis, with trypsin-treated hydrolysates having the highest values. The current work offered a great role of skimmed camel’s milk hydrolysates and the possibilities for using these hydrolysates as food natural preservatives.","PeriodicalId":8803,"journal":{"name":"Benha Veterinary Medical Journal","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Dual functional properties of enzymatically hydrolyzed dromedary milk proteins with improved antioxidant and antibacterial activity\",\"authors\":\"Dina Awad, Osama Ahmed, Samar Baloza, Maha Mamdouh\",\"doi\":\"10.21608/bvmj.2023.228158.1700\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Keywords Dromedary milk has a proven outstanding history of being a nutrient-rich food. Camel milk possesses both medicinal and technological advantages, including antioxidant, antibacterial, and antiparasitic activities. The present investigation is proposed to assess the antibacterial ability and free radical scavenging capacity against Gram-negative bacteria of various enzyme-digested skimmed camel’s milk (SCM) using papain, pepsin, and trypsin at four hydrolysis times at one-hour intervals. An antibacterial growth assay was conducted to detect the antibacterial capacity of the generated various enzymatic camel milk hydrolysates. The results showed that the antibacterial activity of unhydrolyzed intact SCM displayed mild effect against Escherichia coli and no effect against Salmonella enteritidis . Pepsin-treated SCM hydrolysates after 3h hydrolysis significantly enhanced SCM antimicrobial activity against E. coli , and after 2h, hydrolysis produces SCM hydrolysates against Salmonella enteritidis. Pepsin and trypsin SCM-treated hydrolysates after 2h hydrolysis could completely abolish the survival growth rate of Salmonella enteritidis. Additionally, hydrolysates treated with papain SCM after 3 and 4 hours of hydrolysis showed a 100% ability to kill Salmonella enteritidis . Both DPPH method and ABTS assays were employed to evaluate antioxidants. Our findings demonstrated that all SCM hydrolysates gradually acquired strong antioxidant capacity after four hours of hydrolysis, with trypsin-treated hydrolysates having the highest values. The current work offered a great role of skimmed camel’s milk hydrolysates and the possibilities for using these hydrolysates as food natural preservatives.\",\"PeriodicalId\":8803,\"journal\":{\"name\":\"Benha Veterinary Medical Journal\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Benha Veterinary Medical Journal\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.21608/bvmj.2023.228158.1700\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Benha Veterinary Medical Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.21608/bvmj.2023.228158.1700","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Dual functional properties of enzymatically hydrolyzed dromedary milk proteins with improved antioxidant and antibacterial activity
Keywords Dromedary milk has a proven outstanding history of being a nutrient-rich food. Camel milk possesses both medicinal and technological advantages, including antioxidant, antibacterial, and antiparasitic activities. The present investigation is proposed to assess the antibacterial ability and free radical scavenging capacity against Gram-negative bacteria of various enzyme-digested skimmed camel’s milk (SCM) using papain, pepsin, and trypsin at four hydrolysis times at one-hour intervals. An antibacterial growth assay was conducted to detect the antibacterial capacity of the generated various enzymatic camel milk hydrolysates. The results showed that the antibacterial activity of unhydrolyzed intact SCM displayed mild effect against Escherichia coli and no effect against Salmonella enteritidis . Pepsin-treated SCM hydrolysates after 3h hydrolysis significantly enhanced SCM antimicrobial activity against E. coli , and after 2h, hydrolysis produces SCM hydrolysates against Salmonella enteritidis. Pepsin and trypsin SCM-treated hydrolysates after 2h hydrolysis could completely abolish the survival growth rate of Salmonella enteritidis. Additionally, hydrolysates treated with papain SCM after 3 and 4 hours of hydrolysis showed a 100% ability to kill Salmonella enteritidis . Both DPPH method and ABTS assays were employed to evaluate antioxidants. Our findings demonstrated that all SCM hydrolysates gradually acquired strong antioxidant capacity after four hours of hydrolysis, with trypsin-treated hydrolysates having the highest values. The current work offered a great role of skimmed camel’s milk hydrolysates and the possibilities for using these hydrolysates as food natural preservatives.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
Cowpea 11S globulin spray quadruples shrimp shelf-life and maintains freshness Prevalence of some Foodborne Pathogens (FBPs) in imported chilled and frozen beef in Egypt Evaluation of the antidiabetic and hepatoprotective effect of Origanum majorana leaf extract and its nanoparticles in streptozotocin-induced diabetes in rats Ameliorative effect of lemon balm on eltroxin induced-hyperthyroidism in rats Molecular characterization of isolated duck viral hepatitis and its pathogenicity in one day and seven day old ducklings in Qalyubia governorate.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1