低成本的无护套微流控阻抗细胞术在护理点的应用

IF 2.3 4区 工程技术 Q2 INSTRUMENTS & INSTRUMENTATION Microfluidics and Nanofluidics Pub Date : 2023-12-06 DOI:10.1007/s10404-023-02697-6
Seied Ali Hosseini, Sanaz Bahrami, Leila Zolghadr, Nematollah Gheibi
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引用次数: 0

摘要

本研究介绍了一种采用阻抗测量的微流控装置来精确计数悬浮细胞。在此设备开发之前,阻抗细胞术微流控芯片需要使用平面电极和鞘液,这使系统复杂化,或者使用阻碍细胞运动和降低操作效率的小收缩区域。这种新开发的设备能够灵敏和快速的细胞计数,而不需要鞘液或平面电极,使其适用于护理点应用。代替薄膜电极,使用与液体注射相同的针头进行阻抗测量,从而简化了设备。利用分析和计算机辅助模拟设计了该装置的物理参数,以确定灵敏检测人体细胞所需的最大尺寸。模拟研究了流速、电池形状和注射方式对器件性能的影响,并与实验结果进行了比较。最后,对该装置在不同流速和浓度下计数MCF7细胞的能力进行了测试,在1 mL/hr流速下,检测限为每μL 32.3个细胞。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Low-cost sheath-less microfluidic impedance cytometry for point-of-care applications

The present study introduces a microfluidic device that employs impedance measurement to accurately enumerate cells in suspension. Prior to the development of this device, impedance cytometry microfluidic chips necessitated the use of planar electrodes and sheath fluids, which complicated the system, or utilized small constricted regions that impeded cell movement and reduced operational efficiency. This newly developed device is capable of sensitive and rapid cell enumeration without the need for sheath fluid or planar electrodes, making it suitable for point-of-care applications. Instead of thin-film electrodes, the same needles used for liquid injection were implemented for impedance measurement, thus simplifying the device. The physical parameters of the device were designed using analytical and computer-aided simulations to determine the maximum dimensions required for sensitive detection of human cells. Simulations were also employed to investigate the effects of flow rates, cell shape, and injection method on device performance, and results were compared with experimental findings. Finally, this novel device was tested for its ability to count MCF7 cells at various flow rates and concentrations, with a limit of detection of 32.3 cells per μL being achieved in 1 mL/hr flow rate.

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来源期刊
Microfluidics and Nanofluidics
Microfluidics and Nanofluidics 工程技术-纳米科技
CiteScore
4.80
自引率
3.60%
发文量
97
审稿时长
2 months
期刊介绍: Microfluidics and Nanofluidics is an international peer-reviewed journal that aims to publish papers in all aspects of microfluidics, nanofluidics and lab-on-a-chip science and technology. The objectives of the journal are to (1) provide an overview of the current state of the research and development in microfluidics, nanofluidics and lab-on-a-chip devices, (2) improve the fundamental understanding of microfluidic and nanofluidic phenomena, and (3) discuss applications of microfluidics, nanofluidics and lab-on-a-chip devices. Topics covered in this journal include: 1.000 Fundamental principles of micro- and nanoscale phenomena like, flow, mass transport and reactions 3.000 Theoretical models and numerical simulation with experimental and/or analytical proof 4.000 Novel measurement & characterization technologies 5.000 Devices (actuators and sensors) 6.000 New unit-operations for dedicated microfluidic platforms 7.000 Lab-on-a-Chip applications 8.000 Microfabrication technologies and materials Please note, Microfluidics and Nanofluidics does not publish manuscripts studying pure microscale heat transfer since there are many journals that cover this field of research (Journal of Heat Transfer, Journal of Heat and Mass Transfer, Journal of Heat and Fluid Flow, etc.).
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