西藏中长跑运动员从高海拔向低海拔过渡后蛋白质表达的变化:加强耐力训练的意义

IF 2.3 Q2 SPORT SCIENCES Sports Medicine and Health Science Pub Date : 2023-12-20 DOI:10.1016/j.smhs.2023.12.005
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引用次数: 0

摘要

本研究旨在探讨西藏中长跑运动员从高海拔向低海拔过渡后蛋白质表达的差异,并揭示其提高中长跑成绩的分子机制。研究从西藏本土中长跑运动员中选取了11名受试者参加为期8周的赛前运动训练计划,包括在海拔2 560米的康定市进行为期6周的训练,以及随后在海拔360米的乐山市进行为期2周的训练。在康定市开始高海拔运动训练之前和到乐山市海平面之后,对跑步者进行了两次血液样本采集。采用无标记定量方法,对样本中的肽进行了质谱分析。通过蛋白质组分析,确定了不同表达的蛋白质,并预测了它们的生物学功能。21 个样本中共鉴定出 846 个蛋白质,其中 719 个为定量蛋白质。共鉴定出 49 个明显差异表达的蛋白质(p < 0.05),包括 28 个上调 0.2 倍或 21 个下调 0.17 倍的蛋白质。上调蛋白包括囊性纤维化跨膜传导调节因子(CFTR)和碳酸酐酶 I(CAI),由于它们在调节深部组织的氧饱和度方面的作用,这些蛋白尤其引人关注。京都基因组百科全书》(KEGG)通路分析表明,这些蛋白质主要参与调节肌动蛋白细胞骨架、局部粘附、生物素吸收和代谢、免疫系统、癌症和膜运输过程。总之,居住在高海拔地区的藏族中长跑运动员在赛前反复进行的高原-平原交替训练模式中获益匪浅。这种训练模式引起了外周血血浆蛋白(CFTR 和 CAI)的积极变化,而这两种蛋白是与有氧能力相关的生物标志物。在 11 名参赛选手中,有一名女运动员获得了本次比赛 3 000 米跑步项目的金牌,这表明高原平原交替训练模式可提高运动员的有氧能力。
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Protein expression changes in Tibetan middle-to-long distance runners after the transition from high altitude to low altitude: Implications for enhancing endurance training

The study aims to investigate the differences in protein expressions in Xizang's (Tibetan) middle-to-long distance runners after the transition from high altitude to low altitude and reveal the molecular mechanisms underlying their enhanced middle-to-long distance running performance. In the study, eleven subjects were selected from native Tibetan middle-to-long distance runners to participate in an 8-week pre-competition exercise training program consisting of a 6-week training stage in Kangding City at an altitude of 2 560 meters (m) and a subsequent 2-week training stage in Leshan City at an altitude of 360 ​m. Blood samples were collected twice from the runners before beginning altitude exercise training in Kangding and after going to sea level - Leshan City. Using a label-free quantitative method, peptides in the samples were analyzed by mass spectrometry. Proteomic analysis was performed to identify differentially expressed proteins and predict their biological functions. A total of 846 proteins were identified in the 21 samples, including 719 quantified proteins. In total, 49 significantly differentially expressed proteins (p ​< ​0.05) were identified, including twenty-eight 0.2-fold up-regulated proteins or twenty-one 0.17-fold down-regulated proteins. The up-regulated proteins, including cystic fibrosis transmembrane conductance regulator (CFTR) and carbonic anhydrase I (CAI), were of particular interest due to their role in regulating the oxygen saturation in deep tissues. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis indicated that these proteins were mainly involved in regulating actin cytoskeleton, local adhesion, biotin absorption and metabolism, immune system, cancer, and membrane transport processes. In conclusion, Tibetan middle-to-long distance runners who resided in high-altitude areas benefited from repeated plateau-plain alternate training mode during the pre-competition period. The training mode induced positive changes in peripheral blood plasma proteins (CFTR and CAI), the biomarkers associated with aerobic capacity. Among the 11 runners, one female athlete won the gold medal in the 3 000-m running event in this competition, demonstrating that the plateau-plain alternate training mode could enhance the aerobic capacity of athletes.

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来源期刊
Sports Medicine and Health Science
Sports Medicine and Health Science Health Professions-Physical Therapy, Sports Therapy and Rehabilitation
CiteScore
5.50
自引率
0.00%
发文量
36
审稿时长
55 days
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