Shuyuan Zhang , Jiabao Wu , Junqin Chen , Shijia Jun , Yuhong Yuan , Xinyu Dai , Feng Wang , Yue Ma
{"title":"蜡样芽孢杆菌对草莓叶斑病(Neopestalotiopsis clavispora)的生物防治效果","authors":"Shuyuan Zhang , Jiabao Wu , Junqin Chen , Shijia Jun , Yuhong Yuan , Xinyu Dai , Feng Wang , Yue Ma","doi":"10.1016/j.scienta.2024.112841","DOIUrl":null,"url":null,"abstract":"<div><p>With their distinctive flavor, fragrance, and nutritional value, strawberry (<em>Fragaria</em> × <em>ananassa</em> Duch.) fruits are popular worldwide. However, strawberry is susceptible to pathogenic fungi in production, which can severely affect yields. In this study, we found a fungal disease in strawberries in Shenyang. The pathogen was identified from morphological characteristics and molecular data as <em>Neopestalotiopsis clavispora</em>. This fungus causes enormous economic losses in strawberry cultivation and production. It is essential to explore effective methods of controlling this disease, including chemical fungicides and biocontrol agents. This study evaluated the antifungal activity of 17 <em>Bacillus</em> strains isolated from soil against <em>N. clavispora</em>. These <em>Bacillus</em> strains showed significant antagonistic effects, with inhibition rates in the dual culture test of 50.22 %–79.48 %. Among these, <em>Bacillus cereus</em> (Bce-2) had the highest inhibition rate of 79.48 %. The ability of different working concentrations of <em>Bacillus cereus</em> culture solution to inhibit mycelium growth was variable, when the working concentration of <em>Bacillus cereus</em> culture solution was 0 %, 2 %, 4 %, 6 % and 8 %, the mycelium dry weight was 0.05–0.52 g. Furthermore, we optimized the fermentation method and evaluated the ability of the <em>Bacillus cereus</em> fermentation solution (BCFS) to control this disease in the field, the disease index of strawberry plants pre-treated with BCFS was reduced by 57.85 %. BCFS treatment improved the activities of peroxidase (POD), superoxide dismutase (SOD), catalase (CAT) and phenylalanine lyase (PAL). BCFS also prevents strawberry leaf spot disease caused by <em>N. clavispora</em> by increasing the expression of the following genes (<em>WRKY22, WRKY29, ChiB, RbohD, HSP90, Rd19, PR1 and PP2C</em>) in strawberry leaves. This study suggests that <em>Bacillus cereus</em> (Bce-2) may be effective in controlling <em>N. clavispora</em>.</p></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":null,"pages":null},"PeriodicalIF":3.9000,"publicationDate":"2024-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The biological control effect of Bacillus cereus on strawberry leaf spot disease caused by Neopestalotiopsis clavispora\",\"authors\":\"Shuyuan Zhang , Jiabao Wu , Junqin Chen , Shijia Jun , Yuhong Yuan , Xinyu Dai , Feng Wang , Yue Ma\",\"doi\":\"10.1016/j.scienta.2024.112841\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>With their distinctive flavor, fragrance, and nutritional value, strawberry (<em>Fragaria</em> × <em>ananassa</em> Duch.) fruits are popular worldwide. However, strawberry is susceptible to pathogenic fungi in production, which can severely affect yields. In this study, we found a fungal disease in strawberries in Shenyang. The pathogen was identified from morphological characteristics and molecular data as <em>Neopestalotiopsis clavispora</em>. This fungus causes enormous economic losses in strawberry cultivation and production. It is essential to explore effective methods of controlling this disease, including chemical fungicides and biocontrol agents. This study evaluated the antifungal activity of 17 <em>Bacillus</em> strains isolated from soil against <em>N. clavispora</em>. These <em>Bacillus</em> strains showed significant antagonistic effects, with inhibition rates in the dual culture test of 50.22 %–79.48 %. Among these, <em>Bacillus cereus</em> (Bce-2) had the highest inhibition rate of 79.48 %. The ability of different working concentrations of <em>Bacillus cereus</em> culture solution to inhibit mycelium growth was variable, when the working concentration of <em>Bacillus cereus</em> culture solution was 0 %, 2 %, 4 %, 6 % and 8 %, the mycelium dry weight was 0.05–0.52 g. Furthermore, we optimized the fermentation method and evaluated the ability of the <em>Bacillus cereus</em> fermentation solution (BCFS) to control this disease in the field, the disease index of strawberry plants pre-treated with BCFS was reduced by 57.85 %. BCFS treatment improved the activities of peroxidase (POD), superoxide dismutase (SOD), catalase (CAT) and phenylalanine lyase (PAL). BCFS also prevents strawberry leaf spot disease caused by <em>N. clavispora</em> by increasing the expression of the following genes (<em>WRKY22, WRKY29, ChiB, RbohD, HSP90, Rd19, PR1 and PP2C</em>) in strawberry leaves. This study suggests that <em>Bacillus cereus</em> (Bce-2) may be effective in controlling <em>N. clavispora</em>.</p></div>\",\"PeriodicalId\":21679,\"journal\":{\"name\":\"Scientia Horticulturae\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.9000,\"publicationDate\":\"2024-01-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Scientia Horticulturae\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0304423824000025\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"HORTICULTURE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Scientia Horticulturae","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0304423824000025","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"HORTICULTURE","Score":null,"Total":0}
The biological control effect of Bacillus cereus on strawberry leaf spot disease caused by Neopestalotiopsis clavispora
With their distinctive flavor, fragrance, and nutritional value, strawberry (Fragaria × ananassa Duch.) fruits are popular worldwide. However, strawberry is susceptible to pathogenic fungi in production, which can severely affect yields. In this study, we found a fungal disease in strawberries in Shenyang. The pathogen was identified from morphological characteristics and molecular data as Neopestalotiopsis clavispora. This fungus causes enormous economic losses in strawberry cultivation and production. It is essential to explore effective methods of controlling this disease, including chemical fungicides and biocontrol agents. This study evaluated the antifungal activity of 17 Bacillus strains isolated from soil against N. clavispora. These Bacillus strains showed significant antagonistic effects, with inhibition rates in the dual culture test of 50.22 %–79.48 %. Among these, Bacillus cereus (Bce-2) had the highest inhibition rate of 79.48 %. The ability of different working concentrations of Bacillus cereus culture solution to inhibit mycelium growth was variable, when the working concentration of Bacillus cereus culture solution was 0 %, 2 %, 4 %, 6 % and 8 %, the mycelium dry weight was 0.05–0.52 g. Furthermore, we optimized the fermentation method and evaluated the ability of the Bacillus cereus fermentation solution (BCFS) to control this disease in the field, the disease index of strawberry plants pre-treated with BCFS was reduced by 57.85 %. BCFS treatment improved the activities of peroxidase (POD), superoxide dismutase (SOD), catalase (CAT) and phenylalanine lyase (PAL). BCFS also prevents strawberry leaf spot disease caused by N. clavispora by increasing the expression of the following genes (WRKY22, WRKY29, ChiB, RbohD, HSP90, Rd19, PR1 and PP2C) in strawberry leaves. This study suggests that Bacillus cereus (Bce-2) may be effective in controlling N. clavispora.
期刊介绍:
Scientia Horticulturae is an international journal publishing research related to horticultural crops. Articles in the journal deal with open or protected production of vegetables, fruits, edible fungi and ornamentals under temperate, subtropical and tropical conditions. Papers in related areas (biochemistry, micropropagation, soil science, plant breeding, plant physiology, phytopathology, etc.) are considered, if they contain information of direct significance to horticulture. Papers on the technical aspects of horticulture (engineering, crop processing, storage, transport etc.) are accepted for publication only if they relate directly to the living product. In the case of plantation crops, those yielding a product that may be used fresh (e.g. tropical vegetables, citrus, bananas, and other fruits) will be considered, while those papers describing the processing of the product (e.g. rubber, tobacco, and quinine) will not. The scope of the journal includes all horticultural crops but does not include speciality crops such as, medicinal crops or forestry crops, such as bamboo. Basic molecular studies without any direct application in horticulture will not be considered for this journal.