在含维生素 C 的生理盐水保存液中保存的钛的表面特征和体外生物相容性。

IF 4.2 3区 医学 Q2 ENGINEERING, BIOMEDICAL Journal of Materials Science: Materials in Medicine Pub Date : 2024-01-11 DOI:10.1007/s10856-023-06769-5
Wen-Si Zhang, Yao Liu, Shui-Yi Shao, Chang-Qing Shu, Yi-Heng Zhou, Song-Mei Zhang, Jing Qiu
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引用次数: 0

摘要

本研究的目的是探索钛植入物的储存方案,并研究其成骨特性。将商用纯钛(cp-Ti)表面和双蚀刻(SLA)钛表面试样分别在空气、生理盐水、10 mM 含维生素 C(VitC)的生理盐水和 100 mM 含维生素 C 的生理盐水中保存 2 周。用扫描电子显微镜(SEM)观察了钛的表面微观形貌,用拉曼光谱和 X 射线光电子能谱(XPS)分析了试样的表面元素组成,并测试了试样的水接触角和表面粗糙度。用 BCA 试剂盒检测了两种钛表面在不同介质中储存后的蛋白质吸附能力。用 CCK-8、激光共聚焦显微镜(CLSM)和 Western blot 检测了两种钛表面在不同培养基中的增殖、粘附和成骨分化活性。扫描电镜结果表明,空气组的钛表面相对干净,而其他三组的钛表面均可见散落的氯化钠或 VitC 晶体。四组钛表面的微观形态没有明显差异。拉曼光谱在两个实验组的钛表面检测到了 VitC 晶体。XPS、水接触角和表面粗糙度结果表明,在 0.9% NaCl 和两种含 VitC 的生理盐水中储存的 cp-Ti 和 SLA-Ti 的碳污染较少,表面亲水性较高。此外,在两种含 VitC 的生理盐水保存液中保存的 cp-Ti 和 SLA-Ti 表面的蛋白质吸附电位明显提高。体外研究结果表明,在 100 mM 含 VitC 的生理盐水保存液中保存两种钛表面可提高 MC3T3-E1 成骨细胞的细胞粘附、增殖和成骨相关蛋白的表达。总之,在 100 mM 含 VitC 的生理盐水保存液中保存 cp-Ti 和 SLA-Ti 可有效减少碳污染,提高表面亲水性,有利于成骨细胞的成骨分化。
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Surface characteristics and in vitro biocompatibility of titanium preserved in a vitamin C-containing saline storage solution.

The purpose of this study is to explore a storage solution for titanium implants and investigate its osteogenic properties. The commercial pure titanium (cp-Ti) surface and double-etched (SLA) titanium surface specimens were preserved in air, saline, 10 mM Vitamin C (VitC)-containing saline and 100 mM VitC-containing saline storage solutions for 2 weeks. The surface microtopography of titanium was observed by scanning electron microscopy (SEM), the surface elemental compositions of the specimens were analyzed by Raman and X-ray photoelectron spectroscopy (XPS), and water contact angle and surface roughness of the specimens were tested. The protein adsorption capacity of two titanium surfaces after storage in different media was examined by BCA kit. The MC3T3-E1 osteoblasts were cultured on two titanium surfaces after storage in different media, and the proliferation, adhesion and osteogenic differentiation activity of osteoblasts were detected by CCK-8, laser confocal microscope (CLSM) and Western blot. The SEM results indicated that the titanium surfaces of the air group were relatively clean while scattered sodium chloride or VitC crystals were seen on the titanium surfaces of the other three groups. There were no significant differences in the micromorphology of the titanium surfaces among the four groups. Raman spectroscopy detected VitC crystals on the titanium surfaces of two experimental groups. The XPS, water contact angle and surface roughness results suggested that cp-Ti and SLA-Ti stored in 0.9% NaCl and two VitC-containing saline storage solutions possessed less carbon contamination and higher surface hydrophilicity. Moreover, the protein adsorption potentials of cp-Ti and SLA-Ti surfaces were significantly improved under preservation in two VitC-containing saline storage solutions. The results of in vitro study showed that the preservation of two titanium surfaces in 100 mM VitC-containing saline storage solution upregulated the cell adhesion, proliferation, osteogenic related protein expressions of MC3T3-E1 osteoblasts. In conclusion, preservation of cp-Ti and SLA-Ti in 100 mM VitC-containing saline storage solution could effectively reduce carbon contamination and enhance surface hydrophilicity, which was conducive to osteogenic differentiation of osteoblasts.

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来源期刊
Journal of Materials Science: Materials in Medicine
Journal of Materials Science: Materials in Medicine 工程技术-材料科学:生物材料
CiteScore
8.00
自引率
0.00%
发文量
73
审稿时长
3.5 months
期刊介绍: The Journal of Materials Science: Materials in Medicine publishes refereed papers providing significant progress in the application of biomaterials and tissue engineering constructs as medical or dental implants, prostheses and devices. Coverage spans a wide range of topics from basic science to clinical applications, around the theme of materials in medicine and dentistry. The central element is the development of synthetic and natural materials used in orthopaedic, maxillofacial, cardiovascular, neurological, ophthalmic and dental applications. Special biomedical topics include biomaterial synthesis and characterisation, biocompatibility studies, nanomedicine, tissue engineering constructs and cell substrates, regenerative medicine, computer modelling and other advanced experimental methodologies.
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