基于羟基磷灰石的含软骨素酶 ABCI 的纳米系统的合成与评估

IF 3.5 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY AMB Express Pub Date : 2024-02-14 DOI:10.1186/s13568-024-01677-5
Fatemeh Afraei, Sara Daneshjou, Bahareh Dabirmanesh
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引用次数: 0

摘要

从普通变形杆菌(Proteus Vulgaris)中分离出的细菌酶软骨素酶 ABCI(chABCI)对治疗脊髓损伤至关重要。然而,由于其寿命短,这种酶的维持和临床应用受到很大限制。本研究将这种酶固定在羟基磷灰石上,并对其进行了评估,目的是提高 chABCI 的特性和效率。羟基磷灰石颗粒(HAPs)具有良好的生物相容性、形状可控性和高吸附性,是药物输送载体的潜在候选材料。利用纳米尺度可以有效地接触到酶的底物。它以这种方式展示了重要的生物应用能力。研究人员利用场发射枪扫描电子显微镜(FEG-SEM)、X 射线衍射(XRD)、红外光谱(FT-IR)、体外释放研究和细胞毒性测试来表征药物纳米系统的特性。研究结果表明,酶的带电基团与羟基磷灰石纳米颗粒之间形成了静电结合。研究结果还表明,固定在羟基磷灰石上的chABCI具有更易管理药物释放、毒性和副作用最小等有利特性,而且极有可能提高给药效果并减少重复注射的需要。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Synthesis and evaluation of nanosystem containing chondroitinase ABCI based on hydroxyapatite.

The bacterial enzyme chondroitinase ABCI (chABCI), which has been isolated from Proteus Vulgaris, is crucial in the treatment of spinal cord injuries. However, due to its short lifespan, the maintenance and clinical application of this enzyme are very constrained. In this study, the immobilization of this enzyme on hydroxyapatite has been carried out and assessed with the aim of enhancing the characteristics and efficiency of chABCI. Hydroxyapatite particles (HAPs) are a potential candidate for drug-delivery carriers because of their excellent biocompatibility, shape controllability, and high adsorption. The use of the nanometer scale allows efficient access to the enzyme's substrate. It demonstrates important biological application capabilities in this way. Field emission gun-scanning electron microscopy (FEG-SEM), X-ray diffraction (XRD), infrared spectroscopy (FT-IR), in vitro release study, and cytotoxicity test were used to characterize the drug nanosystem's properties. According to the findings, electrostatic bindings was formed between charged groups of the enzyme and hydroxyapatite nanoparticles. The results also demonstrated that immobilized chABCI on hydroxyapatite has beneficial properties, such as more manageable drug release, minimal toxicity and side effects, and a high potential to enhance the efficacy of drug delivery and decrease the need for repeated injections.

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来源期刊
AMB Express
AMB Express BIOTECHNOLOGY & APPLIED MICROBIOLOGY-
CiteScore
7.20
自引率
2.70%
发文量
141
审稿时长
13 weeks
期刊介绍: AMB Express is a high quality journal that brings together research in the area of Applied and Industrial Microbiology with a particular interest in ''White Biotechnology'' and ''Red Biotechnology''. The emphasis is on processes employing microorganisms, eukaryotic cell cultures or enzymes for the biosynthesis, transformation and degradation of compounds. This includes fine and bulk chemicals, polymeric compounds and enzymes or other proteins. Downstream processes are also considered. Integrated processes combining biochemical and chemical processes are also published.
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