使用生物发光耐甲氧西林金黄色葡萄球菌(MRSA)监测金黄色葡萄球菌鼻腔定植小鼠模型。

IF 1.3 4区 农林科学 Q2 VETERINARY SCIENCES Laboratory Animals Pub Date : 2024-06-01 Epub Date: 2024-03-09 DOI:10.1177/00236772231209790
Juliana G da Silva, Juliana Pc Boechat, Bruno Dj Silva, Rodrigo Müller, José Pm Senna
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引用次数: 0

摘要

金黄色葡萄球菌鼻腔携带被认为是感染的一个危险因素,因此制定鼻腔去殖民化策略具有重要意义。尽管小鼠没有金黄色葡萄球菌的自然定植,但它们是金黄色葡萄球菌鼻腔定植的良好模型。小鼠模型易于操作,而且实验室间的可重复性使其适用于鼻腔定植研究。使用生物发光细菌的策略可以监测一段时间内的感染情况,而无需牺牲动物来进行细菌定量。在本研究中,我们使用生物发光菌株(SAP231)评估了金黄色葡萄球菌在三种小鼠品系(BALB/c、C57BL/6 和瑞士韦伯斯特)中的鼻腔定植情况。在体外,观察到可见的生物发光信号发射(BLSE),直到 106 个细菌,并通过 IVIS® 成像系统检测到 104 个细胞。给动物接种一到两个剂量的约 109 菌落总数(CFU)的 SAP231。瑞士韦伯斯特小鼠的定植时间最长,有些动物的BLSE长达140小时。BALB/c 和 C57BL/6 株系在 48 小时内显示出一致的 BLSE 结果,两种剂量接种的瑞士韦伯斯特小鼠的 BLSE 强度更高。对三种不同的图像采集位置进行了评估,侧位和腹背位的结果更好。在失去 BLSE 后,进行了细菌定量,瑞士韦伯斯特小鼠鼻腔中的细菌数量(约 105 CFU)多于其他菌株。我们的研究结果表明,生物发光金黄色葡萄球菌可以监测活体动物的鼻腔定植情况并估算其在 48 小时内的细菌负荷。
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Monitoring Staphylococcus aureus nasal colonization murine model using a bioluminescent methicillin-resistant S. aureus (MRSA).

Staphylococcus aureus nasal carriage is considered a risk factor for infections, and the development of nasal decolonization strategies is highly relevant. Despite they are not naturally colonized by Staphylococcus, mice are a good model for S. aureus nasal colonization. Murine models are easy to manipulate, and inter-laboratory reproducibility makes them suitable for nasal colonization studies. Strategies using bioluminescent bacteria allow for the monitoring of infection over time without the need to sacrifice animals for bacterial quantification. In this study, we evaluated S. aureus nasal colonization in three mouse strains (BALB/c, C57BL/6, and Swiss Webster) using a bioluminescent strain (SAP231). In vitro, a visible Bioluminescent Signal Emission (BLSE) was observed until 106 bacteria and detected by IVIS® imaging system up to 104 cells. Animals were inoculated with one or two doses of approximately 109 colony-forming units (CFU) of SAP231. Swiss Webster mice showed the longest colonization time, with some animals presenting BLSE for up to 140 h. In addition, BLSE was higher in this strain. BALB/c and C57BL/6 strains showed consistent BLSE results for 48 h. BLSE intensity was higher in Swiss Webster inoculated with both doses. Three different positions for image capture were evaluated, with better results for the lateral and ventrodorsal positions. After the loss of BLSE, bacterial quantification was performed, and Swiss Webster mice presented more bacteria in the nasal cavity (approximately 105 CFU) than the other strains. Our results demonstrate that bioluminescent S. aureus allow monitoring of nasal colonization and estimation of the bacterial burden present in live animals until 48 h.

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来源期刊
Laboratory Animals
Laboratory Animals 生物-动物学
CiteScore
4.90
自引率
8.30%
发文量
64
审稿时长
6-12 weeks
期刊介绍: The international journal of laboratory animal science and welfare, Laboratory Animals publishes peer-reviewed original papers and reviews on all aspects of the use of animals in biomedical research. The journal promotes improvements in the welfare or well-being of the animals used, it particularly focuses on research that reduces the number of animals used or which replaces animal models with in vitro alternatives.
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