利用时域漫反射光学光谱分析体外脑、肺和心脏组织热损伤的光学特征

IF 2.9 2区 医学 Q2 BIOCHEMICAL RESEARCH METHODS Biomedical optics express Pub Date : 2024-03-19 DOI:10.1364/boe.517376
Alessandro Bossi, Leonardo Bianchi, Paola Saccomandi, and Antonio Pifferi
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引用次数: 0

摘要

与传统方法相比,热疗法通过加热来治疗肿瘤,大大减少了疼痛、术后并发症和费用。然而,我们仍然需要有效的工具来避免治疗不足或治疗过度,从而指导外科医生进行腹腔镜介入治疗。在这项工作中,我们根据近红外光谱范围(657 至 1107 纳米)内的散射和吸收系数降低情况,研究了活体小牛脑、肺和心脏组织随温度变化的光学特征。这些光谱是通过时域漫射光学测量的,在 43 ℃、60 ℃ 和 80 ℃ 温度下进行阶梯状空间均匀热处理。我们发现散射光谱主要有三种增加,可能是由于胶原蛋白、肌球蛋白和蛋白质二级结构的变性。在 75 °C 之后,我们发现吸收光谱在 770 纳米和 830 纳米处出现了两个新的峰值,这可能是由于形成了一种新的发色团,可能与血红蛋白或肌红蛋白有关。这项研究通过确定热损伤的几个关键标记,标志着利用漫射光学技术控制热疗法向前迈出了重要一步。这将提高实时监测和调整治疗的能力,有望改善肿瘤治疗的效果。
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Optical signatures of thermal damage on ex-vivo brain, lung and heart tissues using time-domain diffuse optical spectroscopy
Thermal therapies treat tumors by means of heat, greatly reducing pain, post-operation complications, and cost as compared to traditional methods. Yet, effective tools to avoid under- or over-treatment are mostly needed, to guide surgeons in laparoscopic interventions. In this work, we investigated the temperature-dependent optical signatures of ex-vivo calf brain, lung, and heart tissues based on the reduced scattering and absorption coefficients in the near-infrared spectral range (657 to 1107 nm). These spectra were measured by time domain diffuse optics, applying a step-like spatially homogeneous thermal treatment at 43 °C, 60 °C, and 80 °C. We found three main increases in scattering spectra, possibly due to the denaturation of collagen, myosin, and the proteins' secondary structure. After 75 °C, we found the rise of two new peaks at 770 and 830 nm in the absorption spectra due to the formation of a new chromophore, possibly related to hemoglobin or myoglobin. This research marks a significant step forward in controlling thermal therapies with diffuse optical techniques by identifying several key markers of thermal damage. This could enhance the ability to monitor and adjust treatment in real-time, promising improved outcomes in tumor therapy.
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来源期刊
Biomedical optics express
Biomedical optics express BIOCHEMICAL RESEARCH METHODS-OPTICS
CiteScore
6.80
自引率
11.80%
发文量
633
审稿时长
1 months
期刊介绍: The journal''s scope encompasses fundamental research, technology development, biomedical studies and clinical applications. BOEx focuses on the leading edge topics in the field, including: Tissue optics and spectroscopy Novel microscopies Optical coherence tomography Diffuse and fluorescence tomography Photoacoustic and multimodal imaging Molecular imaging and therapies Nanophotonic biosensing Optical biophysics/photobiology Microfluidic optical devices Vision research.
期刊最新文献
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