Chengjiao Yu , Xin Sui , Changfeng Miao, Boya Liang, Pengfei Gu, Qiang Li
{"title":"从活性污泥中分离出的 Rhodoligotrophos defluvii lm1T 的低营养特性和基因组分析","authors":"Chengjiao Yu , Xin Sui , Changfeng Miao, Boya Liang, Pengfei Gu, Qiang Li","doi":"10.1016/j.ibiod.2024.105793","DOIUrl":null,"url":null,"abstract":"<div><p>Oligotrophic conditions have important role in the evolution of life and also the study of ecological status and ecological functions of oligotrophic bacteria in such ecosystems. In this study, the oligotrophic characteristics of the experimental strain <em>Rhodoligotrophos defluvii</em> lm1<sup>T</sup> (hereinafter referred to as lm1<sup>T</sup>) isolated from activated sludge were investigated. The results indicated that lm1<sup>T</sup> was able to grow normally in Luria-Bertani (LB) and diluted LB solid agar plates, demonstrating it to be a facultative oligotrophic bacterium. Research about carbon source and genome analysis revealed that lm1<sup>T</sup> lacks 6-phosphofructokinase in the glycolysis process, resulting in the inability to convert glucose-6-phosphate to fructose-6-phosphate, which prevented lm1<sup>T</sup> from utilizing glucose. And in line with that, lm1<sup>T</sup> can utilize 1,6-diphosphofructose, acetate, propionate, and butyrate to meet its growth requirements. lm1<sup>T</sup> possesses a wide range of nitrogen sources utilization, including NaNO<sub>3</sub>, urea, NH<sub>4</sub>Cl, (NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub>, peptone, and yeast extract, enabling its adaptation to various complex environments. Additionally, comparative genomic analysis of lm1<sup>T</sup> and the model strain <em>Rhodoligotrophos appendicifer</em> 120-1<sup>T</sup> revealed that lm1<sup>T</sup> had more genes related to degradation metabolism, which aided its adaptation to complex environments.</p></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":null,"pages":null},"PeriodicalIF":4.1000,"publicationDate":"2024-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Oligotrophic characteristics and genomic analysis of the Rhodoligotrophos defluvii lm1T isolated from activated sludge\",\"authors\":\"Chengjiao Yu , Xin Sui , Changfeng Miao, Boya Liang, Pengfei Gu, Qiang Li\",\"doi\":\"10.1016/j.ibiod.2024.105793\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Oligotrophic conditions have important role in the evolution of life and also the study of ecological status and ecological functions of oligotrophic bacteria in such ecosystems. In this study, the oligotrophic characteristics of the experimental strain <em>Rhodoligotrophos defluvii</em> lm1<sup>T</sup> (hereinafter referred to as lm1<sup>T</sup>) isolated from activated sludge were investigated. The results indicated that lm1<sup>T</sup> was able to grow normally in Luria-Bertani (LB) and diluted LB solid agar plates, demonstrating it to be a facultative oligotrophic bacterium. Research about carbon source and genome analysis revealed that lm1<sup>T</sup> lacks 6-phosphofructokinase in the glycolysis process, resulting in the inability to convert glucose-6-phosphate to fructose-6-phosphate, which prevented lm1<sup>T</sup> from utilizing glucose. And in line with that, lm1<sup>T</sup> can utilize 1,6-diphosphofructose, acetate, propionate, and butyrate to meet its growth requirements. lm1<sup>T</sup> possesses a wide range of nitrogen sources utilization, including NaNO<sub>3</sub>, urea, NH<sub>4</sub>Cl, (NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub>, peptone, and yeast extract, enabling its adaptation to various complex environments. Additionally, comparative genomic analysis of lm1<sup>T</sup> and the model strain <em>Rhodoligotrophos appendicifer</em> 120-1<sup>T</sup> revealed that lm1<sup>T</sup> had more genes related to degradation metabolism, which aided its adaptation to complex environments.</p></div>\",\"PeriodicalId\":13643,\"journal\":{\"name\":\"International Biodeterioration & Biodegradation\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.1000,\"publicationDate\":\"2024-04-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Biodeterioration & Biodegradation\",\"FirstCategoryId\":\"93\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0964830524000647\",\"RegionNum\":2,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Biodeterioration & Biodegradation","FirstCategoryId":"93","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0964830524000647","RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Oligotrophic characteristics and genomic analysis of the Rhodoligotrophos defluvii lm1T isolated from activated sludge
Oligotrophic conditions have important role in the evolution of life and also the study of ecological status and ecological functions of oligotrophic bacteria in such ecosystems. In this study, the oligotrophic characteristics of the experimental strain Rhodoligotrophos defluvii lm1T (hereinafter referred to as lm1T) isolated from activated sludge were investigated. The results indicated that lm1T was able to grow normally in Luria-Bertani (LB) and diluted LB solid agar plates, demonstrating it to be a facultative oligotrophic bacterium. Research about carbon source and genome analysis revealed that lm1T lacks 6-phosphofructokinase in the glycolysis process, resulting in the inability to convert glucose-6-phosphate to fructose-6-phosphate, which prevented lm1T from utilizing glucose. And in line with that, lm1T can utilize 1,6-diphosphofructose, acetate, propionate, and butyrate to meet its growth requirements. lm1T possesses a wide range of nitrogen sources utilization, including NaNO3, urea, NH4Cl, (NH4)2SO4, peptone, and yeast extract, enabling its adaptation to various complex environments. Additionally, comparative genomic analysis of lm1T and the model strain Rhodoligotrophos appendicifer 120-1T revealed that lm1T had more genes related to degradation metabolism, which aided its adaptation to complex environments.
期刊介绍:
International Biodeterioration and Biodegradation publishes original research papers and reviews on the biological causes of deterioration or degradation.