快速开发和大规模生产对仓鼠具有保护效力的 SARS-CoV-2 中和性鸡卵黄抗体。

IF 4.3 2区 生物学 Q1 BIOLOGY Biological Research Pub Date : 2024-05-06 DOI:10.1186/s40659-024-00508-y
Binan Zhao, Haoran Peng, Yanjing Zhang, Jie Zhang, Desheng Kong, Sai Cao, Yan Li, Dan Yang, Chuanwen Sun, Xinyi Pu, Ping Zhao, Yan Xu, Kai Zhao, Liangzhi Xie
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引用次数: 0

摘要

尽管针对 SARS-CoV-2 病毒的疫苗和疗法的开发速度创下了纪录,但并不意味着未来的大流行病也能取得这样的成功。此外,在发展中国家,COVID-19 疫苗的接种率和疗法的应用率仍然很低。快速、低成本地大规模生产抗病毒 IgY 抗体可能是疫苗和疗法开发的一个有吸引力的替代或补充方案。在这篇文章中,我们在 SARS-CoV-2 基因序列公开后的 2 个月内快速制备了 SARS-CoV-2 抗原、免疫母鸡并纯化了 IgY 抗体。我们进一步证明了 IgY 抗体能竞争性地阻断 RBD 与 ACE2 的结合,中和真实的 SARS-CoV-2 病毒,并能有效地保护仓鼠免受 SARS-CoV-2 的挑战,防止体重减轻和肺部病变,这是首次用 IgY 抗体进行的全面研究。大规模生产的过程在大多数发展中国家都很容易实现,因此可以成为我们抗击病毒大流行工具箱中的一个新的重要选择。这项研究可以促进进一步研究、优化和潜在应用 IgY 抗体作为人类和动物的治疗和预防药物。
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Rapid development and mass production of SARS-CoV-2 neutralizing chicken egg yolk antibodies with protective efficacy in hamsters.

Despite the record speed of developing vaccines and therapeutics against the SARS-CoV-2 virus, it is not a given that such success can be secured in future pandemics. In addition, COVID-19 vaccination and application of therapeutics remain low in developing countries. Rapid and low cost mass production of antiviral IgY antibodies could be an attractive alternative or complementary option for vaccine and therapeutic development. In this article, we rapidly produced SARS-CoV-2 antigens, immunized hens and purified IgY antibodies in 2 months after the SARS-CoV-2 gene sequence became public. We further demonstrated that the IgY antibodies competitively block RBD binding to ACE2, neutralize authentic SARS-CoV-2 virus and effectively protect hamsters from SARS-CoV-2 challenge by preventing weight loss and lung pathology, representing the first comprehensive study with IgY antibodies. The process of mass production can be easily implemented in most developing countries and hence could become a new vital option in our toolbox for combating viral pandemics. This study could stimulate further studies, optimization and potential applications of IgY antibodies as therapeutics and prophylactics for human and animals.

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来源期刊
Biological Research
Biological Research 生物-生物学
CiteScore
10.10
自引率
0.00%
发文量
33
审稿时长
>12 weeks
期刊介绍: Biological Research is an open access, peer-reviewed journal that encompasses diverse fields of experimental biology, such as biochemistry, bioinformatics, biotechnology, cell biology, cancer, chemical biology, developmental biology, evolutionary biology, genetics, genomics, immunology, marine biology, microbiology, molecular biology, neuroscience, plant biology, physiology, stem cell research, structural biology and systems biology.
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