雄性大鼠的高脂饮食、微生物组-肠-脑轴信号传导和焦虑样行为。

IF 4.3 2区 生物学 Q1 BIOLOGY Biological Research Pub Date : 2024-05-06 DOI:10.1186/s40659-024-00505-1
Sylvana I S Rendeiro de Noronha, Lauro Angelo Gonçalves de Moraes, James E Hassell, Christopher E Stamper, Mathew R Arnold, Jared D Heinze, Christine L Foxx, Margaret M Lieb, Kristin E Cler, Bree L Karns, Sophia Jaekel, Kelsey M Loupy, Fernanda C S Silva, Deoclécio Alves Chianca-Jr, Christopher A Lowry, Rodrigo Cunha de Menezes
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引用次数: 0

摘要

肥胖(与摄入高脂肪饮食(HFD)有关)和焦虑是生活在现代城市社会中的人的共同特征。最近的研究表明,微生物组-肠道-大脑轴信号传导,包括大脑血清素能系统在高脂饮食与焦虑之间的关系中发挥作用。有证据表明,肠道微生物组和大脑血清素能系统可能在这一反应中共同发挥重要作用。在此,我们对雄性大鼠进行了为期九周的高氟日粮方案,随后分析了肠道微生物组的多样性和群落组成、脑干血清素能基因表达(ph2、htr1a 和 slc6a4)以及与焦虑相关的防御性行为反应。我们的研究表明,摄入高氟日粮会降低肠道微生物组的α多样性并改变其群落组成,从而导致肥胖;增加脑干tph2、htr1a和slc6a4 mRNA的表达,包括在背内侧背侧剑突核(ctRD)尾部的表达,该亚区以前与压力和焦虑相关的行为反应有关;最后,增加焦虑相关的防御性行为反应。与对照组饮食相比,HFD 增加了固着菌/类杆菌的比例,并提高了 Blautia 的相对丰度,降低了 Prevotella 的丰度。我们发现,与对照组饮食相比,HFD 食物中背侧剑突核亚区域的 tph2、htr1a 和 slc6a4 mRNA 表达增加。特定细菌类群与 cDRD 中血清素能基因表达的增加有关。因此,我们认为高密度脂蛋白胆固醇诱导的肥胖与微生物组-肠道-血清素能脑轴信号的改变有关,从而导致大鼠焦虑相关防御性行为反应的增加。
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High-fat diet, microbiome-gut-brain axis signaling, and anxiety-like behavior in male rats.

Obesity, associated with the intake of a high-fat diet (HFD), and anxiety are common among those living in modern urban societies. Recent studies suggest a role of microbiome-gut-brain axis signaling, including a role for brain serotonergic systems in the relationship between HFD and anxiety. Evidence suggests the gut microbiome and the serotonergic brain system together may play an important role in this response. Here we conducted a nine-week HFD protocol in male rats, followed by an analysis of the gut microbiome diversity and community composition, brainstem serotonergic gene expression (tph2, htr1a, and slc6a4), and anxiety-related defensive behavioral responses. We show that HFD intake decreased alpha diversity and altered the community composition of the gut microbiome in association with obesity, increased brainstem tph2, htr1a and slc6a4 mRNA expression, including in the caudal part of the dorsomedial dorsal raphe nucleus (cDRD), a subregion previously associated with stress- and anxiety-related behavioral responses, and, finally, increased anxiety-related defensive behavioral responses. The HFD increased the Firmicutes/Bacteroidetes ratio relative to control diet, as well as higher relative abundances of Blautia, and decreases in Prevotella. We found that tph2, htr1a and slc6a4 mRNA expression were increased in subregions of the dorsal raphe nucleus in the HFD, relative to control diet. Specific bacterial taxa were associated with increased serotonergic gene expression in the cDRD. Thus, we propose that HFD-induced obesity is associated with altered microbiome-gut-serotonergic brain axis signaling, leading to increased anxiety-related defensive behavioral responses in rats.

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来源期刊
Biological Research
Biological Research 生物-生物学
CiteScore
10.10
自引率
0.00%
发文量
33
审稿时长
>12 weeks
期刊介绍: Biological Research is an open access, peer-reviewed journal that encompasses diverse fields of experimental biology, such as biochemistry, bioinformatics, biotechnology, cell biology, cancer, chemical biology, developmental biology, evolutionary biology, genetics, genomics, immunology, marine biology, microbiology, molecular biology, neuroscience, plant biology, physiology, stem cell research, structural biology and systems biology.
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