Pawankumar Rai, Srishti Mehrotra, Vaibhavi Lahane, Akhilesh K. Yadav, Sandeep K. Sharma
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Development of a positive pressure-based instrumentation for efficient solid phase extraction
Solid phase extraction technique is a widely used sample preparation technique for the extraction of components from complex food matrices. However, there are several parameters in SPE that leads to low recovery, and reproducibility, insufficiently clean extracts and evaporation of volatile compounds. These drawbacks can be addressed through the use of innovative techniques and instrumentation that offers improved efficiency and accuracy for isolation of active constituents from food and beverage samples. Here a solid phase extraction methodology and extraction assembly has been developed for extracting compounds from orange fruit juice, followed by analysis using a LC-HRMS system. The methodology and assembly eliminate the evaporation step, thus facilitating the analysis of wide range of volatile and non-volatile compounds without the risk of their evaporation, making it particularly suitable for thermo-sensitive compounds. Also, the elute can be used for further analysis using LC-HRMS. The effectiveness of the designed extraction assembly and technique was demonstrated by comparing the procedure with the available conventional extraction assemblies, highlighting the advantages of the developed method.
期刊介绍:
The Journal of Food Science and Technology (JFST) is the official publication of the Association of Food Scientists and Technologists of India (AFSTI). This monthly publishes peer-reviewed research papers and reviews in all branches of science, technology, packaging and engineering of foods and food products. Special emphasis is given to fundamental and applied research findings that have potential for enhancing product quality, extend shelf life of fresh and processed food products and improve process efficiency. Critical reviews on new perspectives in food handling and processing, innovative and emerging technologies and trends and future research in food products and food industry byproducts are also welcome. The journal also publishes book reviews relevant to all aspects of food science, technology and engineering.