通过反复 pH 值冲击提高枯草芽孢杆菌的聚-γ-L-二氨基丁酸产量

IF 3.5 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Bioprocess and Biosystems Engineering Pub Date : 2024-09-01 Epub Date: 2024-06-21 DOI:10.1007/s00449-024-03050-z
Shu Li, Nan Wang, Xiaoting Li
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引用次数: 0

摘要

本研究探讨了 pH 值对枯草芽孢杆菌批量发酵生产聚-γ-L-二氨基丁酸(γ-PAB)的影响。在未控制 pH 值的自然发酵中,pH 值在 18 小时内从初始的 7.0 降至 3.0,γ-PAB 产量为 428.6 mg/L。在控制 pH 值的发酵中,普米氏菌在较高的 pH 值下倾向于增殖,而在较低的 pH 值下有利于γ-PAB 的合成,其中γ-PAB 生成的最佳 pH 值为 4.2,γ-PAB 产量达到 2284.5 mg/L。在预发酵阶段采用持续 9 小时的 pH 震荡策略,得到的 B. pumilus 生物量(OD600)和γ-PAB 产量分别为 61.3 和 2794.6 mg/L,比未进行 pH 震荡的批次发酵分别高出 10.8%和 22.4%。随后的重复 pH 震荡发酵表明,可以进一步提高生产率,最终 OD600 达到 65.1,γ-PAB 产量高达 3482.3 mg/L,与单次 pH 震荡发酵相比,分别提高了 6.2% 和 17.1%。该研究表明,普米氏菌能在次优pH条件下合成更多的γ-PAB,为调节γ-PAB的合成提供了一种新方法。
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Enhancement of poly‑γ‑L‑diaminobutanoic acid production in Bacillus pumilus by repeated pH shocks.

This study investigated the effect of pH on poly-γ-L-diaminobutanoic acid (γ-PAB) production by Bacillus pumilus in batch fermentation. In the natural fermentation where pH was not controlled, pH decreased from initial 7.0 to 3.0 in 18 h and γ-PAB production was 428.6 mg/L. In the pH-controlled fermentation, B. pumilus tended to proliferation at higher pH, while γ-PAB synthesis was favorable at lower pH, in which the optimal pH for γ-PAB production was 4.2, and γ-PAB yield reached 2284.5 mg/L. Adopting a pH shock strategy which lasted 9 h in the pre-fermentation phase, biomass (OD600) and γ-PAB yield of B. pumilus were obtained as 61.3 and 2794.6 mg/L, respectively, which were 10.8% and 22.4% higher than those in batch fermentation without pH shock. Subsequent fermentation of repeated pH shocks showed that a further higher productivity could be achieved, in which the final OD600 reached 65.1, and γ-PAB production reached as high as 3482.3 mg/L, which were increased by 6.2% and 17.1% compared with those in single pH shock, respectively. This study demonstrated that B. pumilus can synthesize more γ-PAB at suboptimal pH and provided a novel approach to regulate γ-PAB synthesis.

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来源期刊
Bioprocess and Biosystems Engineering
Bioprocess and Biosystems Engineering 工程技术-工程:化工
CiteScore
7.90
自引率
2.60%
发文量
147
审稿时长
2.6 months
期刊介绍: Bioprocess and Biosystems Engineering provides an international peer-reviewed forum to facilitate the discussion between engineering and biological science to find efficient solutions in the development and improvement of bioprocesses. The aim of the journal is to focus more attention on the multidisciplinary approaches for integrative bioprocess design. Of special interest are the rational manipulation of biosystems through metabolic engineering techniques to provide new biocatalysts as well as the model based design of bioprocesses (up-stream processing, bioreactor operation and downstream processing) that will lead to new and sustainable production processes. Contributions are targeted at new approaches for rational and evolutive design of cellular systems by taking into account the environment and constraints of technical production processes, integration of recombinant technology and process design, as well as new hybrid intersections such as bioinformatics and process systems engineering. Manuscripts concerning the design, simulation, experimental validation, control, and economic as well as ecological evaluation of novel processes using biosystems or parts thereof (e.g., enzymes, microorganisms, mammalian cells, plant cells, or tissue), their related products, or technical devices are also encouraged. The Editors will consider papers for publication based on novelty, their impact on biotechnological production and their contribution to the advancement of bioprocess and biosystems engineering science. Submission of papers dealing with routine aspects of bioprocess engineering (e.g., routine application of established methodologies, and description of established equipment) are discouraged.
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