细菌 c-di-GMP 通过 STING 受体触发贻贝幼虫的变态反应

IF 7.8 1区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY npj Biofilms and Microbiomes Pub Date : 2024-06-20 DOI:10.1038/s41522-024-00523-7
Xiao-Meng Hu, Lihua Peng, Yuyi Wang, Fan Ma, Yu Tao, Xiao Liang, Jin-Long Yang
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引用次数: 0

摘要

几乎所有海洋无脊椎动物都能观察到细菌诱导的变态过程。然而,细菌调控幼虫-幼体变态的机制仍然未知。在这里,我们验证了一种假设,即 c-di-GMP(一种普遍存在的细菌第二信使分子)通过干扰素基因刺激器(STING)受体直接触发软体动物珊瑚贻贝的幼体变态。我们发现,删除 c-di-GMP 合成基因会导致 c-di-GMP 水平降低,并降低幼虫变态过程中的生物膜诱导活性,同时细胞外聚合物质也会发生变化。此外,从测试的不同海洋细菌中提取的 c-di-GMP 都对幼虫变态具有诱导活性。同时,通过药理学和分子实验,我们证明了M. coruscus STING(McSTING)通过与c-di-GMP结合参与了幼虫的变态过程。我们的发现揭示了细菌 c-di-GMP 触发贻贝幼虫变态转变的新作用,并扩展了细菌与海洋生态系统中宿主发育相互作用的知识。
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Bacterial c-di-GMP triggers metamorphosis of mussel larvae through a STING receptor.

Bacteria induced metamorphosis observed in nearly all marine invertebrates. However, the mechanism of bacteria regulating the larvae-juvenile metamorphosis remains unknown. Here, we test the hypothesis that c-di-GMP, a ubiquitous bacterial second-messenger molecule, directly triggers the mollusc Mytilus coruscus larval metamorphosis via the stimulator of interferon genes (STING) receptor. We determined that the deletion of c-di-GMP synthesis genes resulted in reduced c-di-GMP levels and biofilm-inducing activity on larval metamorphosis, accompanied by alterations in extracellular polymeric substances. Additionally, c-di-GMP extracted from tested varying marine bacteria all exhibited inducing activity on larval metamorphosis. Simultaneously, through pharmacological and molecular experiments, we demonstrated that M. coruscus STING (McSTING) participates in larval metamorphosis by binding with c-di-GMP. Our findings reveal that new role of bacterial c-di-GMP that triggers mussel larval metamorphosis transition, and extend knowledge in the interaction of bacteria and host development in marine ecosystems.

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来源期刊
npj Biofilms and Microbiomes
npj Biofilms and Microbiomes Immunology and Microbiology-Microbiology
CiteScore
12.10
自引率
3.30%
发文量
91
审稿时长
9 weeks
期刊介绍: npj Biofilms and Microbiomes is a comprehensive platform that promotes research on biofilms and microbiomes across various scientific disciplines. The journal facilitates cross-disciplinary discussions to enhance our understanding of the biology, ecology, and communal functions of biofilms, populations, and communities. It also focuses on applications in the medical, environmental, and engineering domains. The scope of the journal encompasses all aspects of the field, ranging from cell-cell communication and single cell interactions to the microbiomes of humans, animals, plants, and natural and built environments. The journal also welcomes research on the virome, phageome, mycome, and fungome. It publishes both applied science and theoretical work. As an open access and interdisciplinary journal, its primary goal is to publish significant scientific advancements in microbial biofilms and microbiomes. The journal enables discussions that span multiple disciplines and contributes to our understanding of the social behavior of microbial biofilm populations and communities, and their impact on life, human health, and the environment.
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