使用光学相干断层成像技术对人类视网膜神经节细胞进行活体成像，无需自适应光学技术

IF 2.9 2区医学 Q2 BIOCHEMICAL RESEARCH METHODS Biomedical optics express Pub Date : 2024-06-28 DOI:10.1364/boe.533249

Furu Zhang, Katherine Kovalick, Achyut Raghavendra, Somayyeh Soltanian-Zadeh, Sina Farsiu, Daniel X. Hammer, Zhuolin Liu

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引用次数: 0

摘要

视网膜神经节细胞在人类视觉中发挥着重要作用，它们的退化会导致青光眼和其他神经退行性疾病。对活体视网膜中的这些细胞进行成像可大大提高青光眼的诊断和治疗水平。然而，由于神经节细胞体是半透明的，而且在神经节细胞层（GCL）内排列紧密，因此只能通过精密的研究级自适应光学（AO）系统才能成功成像。我们首次证明，使用非自适应光学相干断层扫描（OCT）设备，通过最佳参数配置和后处理，可以分辨 GCL 体节并量化细胞形态。

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In vivo imaging of human retinal ganglion cells using optical coherence tomography without adaptive optics

Retinal ganglion cells play an important role in human vision, and their degeneration results in glaucoma and other neurodegenerative diseases. Imaging these cells in the living human retina can greatly improve the diagnosis and treatment of glaucoma. However, owing to their translucent soma and tight packing arrangement within the ganglion cell layer (GCL), successful imaging has only been achieved with sophisticated research-grade adaptive optics (AO) systems. For the first time we demonstrate that GCL somas can be resolved and cell morphology can be quantified using non-AO optical coherence tomography (OCT) devices with optimal parameter configuration and post-processing.

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来源期刊

Biomedical optics express BIOCHEMICAL RESEARCH METHODS-OPTICS

CiteScore

6.80

自引率

11.80%

发文量

633

审稿时长

1 months

期刊介绍： The journal''s scope encompasses fundamental research, technology development, biomedical studies and clinical applications. BOEx focuses on the leading edge topics in the field, including: Tissue optics and spectroscopy Novel microscopies Optical coherence tomography Diffuse and fluorescence tomography Photoacoustic and multimodal imaging Molecular imaging and therapies Nanophotonic biosensing Optical biophysics/photobiology Microfluidic optical devices Vision research.