{"title":"用金装饰氧化铁/碳点修饰的玻璃碳电极,用于光助伏安法检测抗生素耐药微生物粪肠球菌","authors":"","doi":"10.1016/j.biosx.2024.100532","DOIUrl":null,"url":null,"abstract":"<div><p>Detecting bacteria is essential in managing significant health concerns as it enables timely intervention, reducing complications and improving patient outcomes, particularly in treating common infections that necessitate precise identification for effective symptom management. Enterococcus species represent a notable threat in hospital-acquired infections and urinary tract infections (UTIs), given the increasing prevalence of strains resistant to multiple antibiotics, unresponsive to standard therapies, and carrying various virulence factors. Traditional approaches to identifying <em>Enterococcus faecalis</em> (<em>E. faecalis</em>) have limitations, including prolonged processing times, limited sensitivity, and the potential for false positive results. While Polymerase Chain Reaction (PCR) is a valuable tool, it is susceptible to contamination and variations in DNA concentration. The emerging technique of Photoelectrochemical (PEC) holds promise for enhancing <em>E. faecalis</em> detection by leveraging photogenerated electrons and holes. This study introduces a rapid and precise approach utilizing a light-assisted electrochemical biosensor featuring a glassy carbon electrode modified with a nanocomposite of gold-coated iron oxide and carbon dots (Au@Fe<sub>3</sub>O<sub>4</sub>/CDs). The nanocomposite was successfully synthesized and underwent thorough characterization. The investigation has a detection range from 1 to 14 CFU mL<sup>−1</sup>, along with a notably low limit of detection (LOD: 3 CFU mL<sup>−1</sup>, LOQ: 10 CFU mL<sup>−1</sup>). Rigorous examination of real-world samples such as food, water, and soil demonstrated exceptional specificity, reproducibility, and long-term stability of the sensor. The applications of the Au@Fe<sub>3</sub>O<sub>4</sub>/CDs nanocomposite in PEC processes underscore the potential of this innovative approach in addressing health concerns associated with bacterial infections and delivering real-time impacts for both healthcare and environmental domains.</p></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":null,"pages":null},"PeriodicalIF":10.6100,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590137024000967/pdfft?md5=34de78549f37917f1dd49d1a65e225d3&pid=1-s2.0-S2590137024000967-main.pdf","citationCount":"0","resultStr":"{\"title\":\"A glassy carbon electrode modified with gold decorated iron oxide/ carbon dots for light assisted voltammetric detection of antibiotic resistant microbe Enterococcus faecalis\",\"authors\":\"\",\"doi\":\"10.1016/j.biosx.2024.100532\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Detecting bacteria is essential in managing significant health concerns as it enables timely intervention, reducing complications and improving patient outcomes, particularly in treating common infections that necessitate precise identification for effective symptom management. Enterococcus species represent a notable threat in hospital-acquired infections and urinary tract infections (UTIs), given the increasing prevalence of strains resistant to multiple antibiotics, unresponsive to standard therapies, and carrying various virulence factors. Traditional approaches to identifying <em>Enterococcus faecalis</em> (<em>E. faecalis</em>) have limitations, including prolonged processing times, limited sensitivity, and the potential for false positive results. While Polymerase Chain Reaction (PCR) is a valuable tool, it is susceptible to contamination and variations in DNA concentration. The emerging technique of Photoelectrochemical (PEC) holds promise for enhancing <em>E. faecalis</em> detection by leveraging photogenerated electrons and holes. This study introduces a rapid and precise approach utilizing a light-assisted electrochemical biosensor featuring a glassy carbon electrode modified with a nanocomposite of gold-coated iron oxide and carbon dots (Au@Fe<sub>3</sub>O<sub>4</sub>/CDs). The nanocomposite was successfully synthesized and underwent thorough characterization. The investigation has a detection range from 1 to 14 CFU mL<sup>−1</sup>, along with a notably low limit of detection (LOD: 3 CFU mL<sup>−1</sup>, LOQ: 10 CFU mL<sup>−1</sup>). Rigorous examination of real-world samples such as food, water, and soil demonstrated exceptional specificity, reproducibility, and long-term stability of the sensor. The applications of the Au@Fe<sub>3</sub>O<sub>4</sub>/CDs nanocomposite in PEC processes underscore the potential of this innovative approach in addressing health concerns associated with bacterial infections and delivering real-time impacts for both healthcare and environmental domains.</p></div>\",\"PeriodicalId\":260,\"journal\":{\"name\":\"Biosensors and Bioelectronics: X\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":10.6100,\"publicationDate\":\"2024-08-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S2590137024000967/pdfft?md5=34de78549f37917f1dd49d1a65e225d3&pid=1-s2.0-S2590137024000967-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biosensors and Bioelectronics: X\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2590137024000967\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Biochemistry, Genetics and Molecular Biology\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biosensors and Bioelectronics: X","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2590137024000967","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
A glassy carbon electrode modified with gold decorated iron oxide/ carbon dots for light assisted voltammetric detection of antibiotic resistant microbe Enterococcus faecalis
Detecting bacteria is essential in managing significant health concerns as it enables timely intervention, reducing complications and improving patient outcomes, particularly in treating common infections that necessitate precise identification for effective symptom management. Enterococcus species represent a notable threat in hospital-acquired infections and urinary tract infections (UTIs), given the increasing prevalence of strains resistant to multiple antibiotics, unresponsive to standard therapies, and carrying various virulence factors. Traditional approaches to identifying Enterococcus faecalis (E. faecalis) have limitations, including prolonged processing times, limited sensitivity, and the potential for false positive results. While Polymerase Chain Reaction (PCR) is a valuable tool, it is susceptible to contamination and variations in DNA concentration. The emerging technique of Photoelectrochemical (PEC) holds promise for enhancing E. faecalis detection by leveraging photogenerated electrons and holes. This study introduces a rapid and precise approach utilizing a light-assisted electrochemical biosensor featuring a glassy carbon electrode modified with a nanocomposite of gold-coated iron oxide and carbon dots (Au@Fe3O4/CDs). The nanocomposite was successfully synthesized and underwent thorough characterization. The investigation has a detection range from 1 to 14 CFU mL−1, along with a notably low limit of detection (LOD: 3 CFU mL−1, LOQ: 10 CFU mL−1). Rigorous examination of real-world samples such as food, water, and soil demonstrated exceptional specificity, reproducibility, and long-term stability of the sensor. The applications of the Au@Fe3O4/CDs nanocomposite in PEC processes underscore the potential of this innovative approach in addressing health concerns associated with bacterial infections and delivering real-time impacts for both healthcare and environmental domains.
期刊介绍:
Biosensors and Bioelectronics: X, an open-access companion journal of Biosensors and Bioelectronics, boasts a 2020 Impact Factor of 10.61 (Journal Citation Reports, Clarivate Analytics 2021). Offering authors the opportunity to share their innovative work freely and globally, Biosensors and Bioelectronics: X aims to be a timely and permanent source of information. The journal publishes original research papers, review articles, communications, editorial highlights, perspectives, opinions, and commentaries at the intersection of technological advancements and high-impact applications. Manuscripts submitted to Biosensors and Bioelectronics: X are assessed based on originality and innovation in technology development or applications, aligning with the journal's goal to cater to a broad audience interested in this dynamic field.