用金装饰氧化铁/碳点修饰的玻璃碳电极,用于光助伏安法检测抗生素耐药微生物粪肠球菌

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology Biosensors and Bioelectronics: X Pub Date : 2024-08-22 DOI:10.1016/j.biosx.2024.100532
Shobana Babu , Renugadevi Kathirvel , Prakash Periakaruppan
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引用次数: 0

摘要

检测细菌对管理重大健康问题至关重要,因为它能及时干预、减少并发症并改善患者的治疗效果,尤其是在治疗常见感染时,需要精确识别才能有效对症治疗。在医院获得性感染和尿路感染(UTI)中,肠球菌是一个显著的威胁,因为对多种抗生素耐药、对标准疗法无反应、携带各种毒力因子的菌株越来越多。鉴别粪肠球菌(E. faecalis)的传统方法有其局限性,包括处理时间长、灵敏度有限以及可能出现假阳性结果。聚合酶链式反应 (PCR) 是一种有价值的工具,但容易受到污染和 DNA 浓度变化的影响。新兴的光电化学(PEC)技术有望利用光产生的电子和空穴来提高粪肠球菌的检测能力。本研究介绍了一种利用光辅助电化学生物传感器的快速而精确的方法,其特点是在玻璃碳电极上修饰了金包覆氧化铁和碳点的纳米复合材料(Au@Fe3O4/CDs)。该纳米复合材料已成功合成,并进行了全面的表征。该研究的检测范围为 1 至 14 CFU mL-1,检测限(LOD:3 CFU mL-1,LOQ:10 CFU mL-1)明显较低。对食物、水和土壤等实际样品的严格检测表明,该传感器具有极高的特异性、再现性和长期稳定性。Au@Fe3O4/CDs 纳米复合材料在 PEC 过程中的应用凸显了这种创新方法在解决与细菌感染相关的健康问题以及为医疗保健和环境领域提供实时影响方面的潜力。
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A glassy carbon electrode modified with gold decorated iron oxide/ carbon dots for light assisted voltammetric detection of antibiotic resistant microbe Enterococcus faecalis

Detecting bacteria is essential in managing significant health concerns as it enables timely intervention, reducing complications and improving patient outcomes, particularly in treating common infections that necessitate precise identification for effective symptom management. Enterococcus species represent a notable threat in hospital-acquired infections and urinary tract infections (UTIs), given the increasing prevalence of strains resistant to multiple antibiotics, unresponsive to standard therapies, and carrying various virulence factors. Traditional approaches to identifying Enterococcus faecalis (E. faecalis) have limitations, including prolonged processing times, limited sensitivity, and the potential for false positive results. While Polymerase Chain Reaction (PCR) is a valuable tool, it is susceptible to contamination and variations in DNA concentration. The emerging technique of Photoelectrochemical (PEC) holds promise for enhancing E. faecalis detection by leveraging photogenerated electrons and holes. This study introduces a rapid and precise approach utilizing a light-assisted electrochemical biosensor featuring a glassy carbon electrode modified with a nanocomposite of gold-coated iron oxide and carbon dots (Au@Fe3O4/CDs). The nanocomposite was successfully synthesized and underwent thorough characterization. The investigation has a detection range from 1 to 14 CFU mL−1, along with a notably low limit of detection (LOD: 3 CFU mL−1, LOQ: 10 CFU mL−1). Rigorous examination of real-world samples such as food, water, and soil demonstrated exceptional specificity, reproducibility, and long-term stability of the sensor. The applications of the Au@Fe3O4/CDs nanocomposite in PEC processes underscore the potential of this innovative approach in addressing health concerns associated with bacterial infections and delivering real-time impacts for both healthcare and environmental domains.

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来源期刊
Biosensors and Bioelectronics: X
Biosensors and Bioelectronics: X Biochemistry, Genetics and Molecular Biology-Biophysics
CiteScore
4.60
自引率
0.00%
发文量
166
审稿时长
54 days
期刊介绍: Biosensors and Bioelectronics: X, an open-access companion journal of Biosensors and Bioelectronics, boasts a 2020 Impact Factor of 10.61 (Journal Citation Reports, Clarivate Analytics 2021). Offering authors the opportunity to share their innovative work freely and globally, Biosensors and Bioelectronics: X aims to be a timely and permanent source of information. The journal publishes original research papers, review articles, communications, editorial highlights, perspectives, opinions, and commentaries at the intersection of technological advancements and high-impact applications. Manuscripts submitted to Biosensors and Bioelectronics: X are assessed based on originality and innovation in technology development or applications, aligning with the journal's goal to cater to a broad audience interested in this dynamic field.
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