吸毒者大鼠戊型肝炎病毒(Rocahepevirus ratti)血清学和分子学调查。

IF 8.4 2区 医学 Q1 IMMUNOLOGY Emerging Microbes & Infections Pub Date : 2024-12-01 Epub Date: 2024-09-04 DOI:10.1080/22221751.2024.2396865
Maria Casares-Jimenez, Diana Corona-Mata, Transito Garcia-Garcia, Leticia Manchado-Lopez, Lucia Rios-Muñoz, Maria de Guia-Castro, Pedro Lopez-Lopez, David Caceres-Anillo, Angela Camacho, Javier Caballero-Gomez, Ignacio Perez-Valero, Marina Gallo-Marin, Ana Belen Perez, Rainer G Ulrich, Antonio Rivero-Juarez, Antonio Rivero
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引用次数: 0

摘要

摘要大鼠戊型肝炎病毒(ratHEV)是一种新出现的人畜共患急性肝炎病因。由于血清流行率研究很少,高危人群几乎不为人知。由于血液传播感染经常发生在吸毒者身上,而吸毒者又因缺乏住房和无家可归而特别容易受到感染,因此这部分人群是应重点评估的戊型肝炎病毒感染人群。因此,本研究旨在评估作为潜在高危人群的吸毒者的鼠HEV血清流行率和RNA检出率。我们设计了一项回顾性研究,涉及参加戒毒康复中心的人员。通过使用 ELISA 和点印迹 (DB) 法检测特异性抗体来评估是否暴露于大鼠 HEV,通过使用 RT-qPCR 检测大鼠 HEV RNA 来评估是否存在活动性感染。研究共纳入 341 人,其中男性最多(67.7%),平均年龄 45 岁。共有 17 人检测出特异性 IgG 抗体(4.6%;95% CI;3.1% - 7.9%)。发现了一例活动性鼠HEV感染病例(0.3%;95% CI:0.1% - 1.8%)。这是一名 57 岁的无家可归妇女,经济来源有限,曾通过肠外途径积极吸食可卡因和海洛因。总之,我们在吸毒者中发现了潜在的 ratHEV 暴露。为了更准确地评估高危人群和传播途径,有必要对吸毒者和适当的对照组进行有针对性的研究。
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Serological and molecular survey of rat hepatitis E virus (Rocahepevirus ratti) in drug users.

ABSTRACTRat hepatitis E virus (ratHEV) is an emerging cause of acute hepatitis of zoonotic origin. Since seroprevalence studies are scarce, at-risk groups are almost unknown. Because blood-borne infections frequently occur in people with drug use, who are particularly vulnerable to infection due to lack of housing and homelessness, this population constitutes a priority in which ratHEV infection should be evaluated. Therefore, the aim of this study was to evaluate the ratHEV seroprevalence and RNA detection rate in drug users as a potential at-risk population. We designed a retrospective study involving individuals that attended drug rehabilitation centres. Exposure to ratHEV was assessed by specific antibody detection using ELISA and dot blot (DB) assay and the presence of active infection by ratHEV RNA detection using RT-qPCR. Three-hundred and forty-one individuals were included, the most of them being men (67.7%) with an average age of 45 years. A total of 17 individuals showed specific IgG antibodies against ratHEV (4.6%; 95% CI; 3.1%-7.9%). One case of active ratHEV infection was identified (0.3%; 95% CI: 0.1%-1.8%). This was a 57-year-old homeless woman with limited financial resources, who had active cocaine and heroin use via parenteral route. In conclusion, we identified a potential exposure to ratHEV among drug users. Targeted studies in drug users with proper control groups are necessary to evaluate high-risk populations and transmission routes more accurately.

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来源期刊
Emerging Microbes & Infections
Emerging Microbes & Infections IMMUNOLOGY-MICROBIOLOGY
CiteScore
26.20
自引率
2.30%
发文量
276
审稿时长
20 weeks
期刊介绍: Emerging Microbes & Infections is a peer-reviewed, open-access journal dedicated to publishing research at the intersection of emerging immunology and microbiology viruses. The journal's mission is to share information on microbes and infections, particularly those gaining significance in both biological and clinical realms due to increased pathogenic frequency. Emerging Microbes & Infections is committed to bridging the scientific gap between developed and developing countries. This journal addresses topics of critical biological and clinical importance, including but not limited to: - Epidemic surveillance - Clinical manifestations - Diagnosis and management - Cellular and molecular pathogenesis - Innate and acquired immune responses between emerging microbes and their hosts - Drug discovery - Vaccine development research Emerging Microbes & Infections invites submissions of original research articles, review articles, letters, and commentaries, fostering a platform for the dissemination of impactful research in the field.
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