Aline Zaparte, Courtney J Christopher, Connie Arnold, Lauren Richey, Adairre Castille, Kyle Mistretta, Christopher M Taylor, Huiyi Lin, Steve Nelson, John P Kirwan, John W Apolzan, Shawn R Campagna, David A Welsh
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引用次数: 0
摘要
电子烟(吸食电子烟)的普及率急剧上升,在青少年和年轻成年人中造成了公共健康危机。长期吸烟会诱发肠道炎症,并通过产生促炎症分子硫化氢(H2S)降低肠道屏障功能。这对艾滋病病毒感染者(PWH)来说尤其令人担忧,因为他们已经面临免疫功能受损的问题,而且患代谢失调、糖尿病和慢性肝病的风险更高。此外,艾滋病病毒感染者的行为也不健康,因此了解与该人群吸烟相关的全身代谢失调和病理生理机制至关重要。在此,我们采用基于液相色谱-质谱联用仪(LC-MS)的代谢组学方法,研究了吸食(7 人)和吸食可燃烟草/大麻(6 人)的公共卫生人员与不吸食或不吸烟的对照组参与者(10 人)相比,其上呼吸道、血液循环和肠道代谢概况。这项提出假设的探索性研究揭示了嘌呤、神经递质和维生素 B 代谢的系统性改变,以及与吸烟和吸食可燃烟草/大麻有关的炎症通路和隐硫循环的组织特异性改变。此外,这项研究首次将微生物衍生代谢物2,3-二羟基丙烷-1-磺酸(DHPS)与吸食(烟草和大麻)诱导的肠道代谢失衡联系起来。这些发现强调了确定生理变化的全部生物学和临床意义以及与吸食大麻相关的风险的重要性。
Effects of E-Cigarettes on the Lung and Systemic Metabolome in People with HIV.
The popularity of e-cigarettes (vaping) has soared, creating a public health crisis among teens and young adults. Chronic vaping can induce gut inflammation and reduce intestinal barrier function through the production of the proinflammatory molecule hydrogen sulfide (H2S). This is particularly concerning for people with HIV (PWH) as they already face impaired immune function and are at a higher risk for metabolic dysregulation, diabetes, and chronic liver disease. Furthermore, PWH experience unhealthy behaviors, making it crucial to understand the systemic metabolic dysregulation and pathophysiological mechanisms associated with vaping in this population. Here, we employed liquid chromatography-mass spectrometry (LC-MS)-based metabolomics to investigate the upper respiratory, circulation, and gut metabolic profiles of PWH who vape (n = 7) and smoke combustible tobacco/marijuana (n = 6) compared to control participants who did not vape or smoke (n = 10). This hypothesis-generating exploratory study revealed systemic alterations in purine, neurotransmitter, and vitamin B metabolisms and tissue-specific changes in inflammatory pathways and cryptic sulfur cycling associated with vaping and combustible tobacco/marijuana smoking in PWH. In addition, this study provides the first link between microbial-derived metabolite 2,3-dihydroxypropane-1-sulfonate (DHPS) and vaping/smoking (tobacco and marijuana)-induced metabolic dyshomeostasis in the gut. These findings highlight the importance of identifying the full biological and clinical significance of the physiological changes and risks associated with vaping.
MetabolitesBiochemistry, Genetics and Molecular Biology-Molecular Biology
CiteScore
5.70
自引率
7.30%
发文量
1070
审稿时长
17.17 days
期刊介绍:
Metabolites (ISSN 2218-1989) is an international, peer-reviewed open access journal of metabolism and metabolomics. Metabolites publishes original research articles and review articles in all molecular aspects of metabolism relevant to the fields of metabolomics, metabolic biochemistry, computational and systems biology, biotechnology and medicine, with a particular focus on the biological roles of metabolites and small molecule biomarkers. Metabolites encourages scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on article length. Sufficient experimental details must be provided to enable the results to be accurately reproduced. Electronic material representing additional figures, materials and methods explanation, or supporting results and evidence can be submitted with the main manuscript as supplementary material.