凝血和纤维蛋白溶解的整合:血小板和因子 XIIIa 的核心作用。

IF 3.8 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Bioscience Reports Pub Date : 2024-09-25 DOI:10.1042/BSR20240332
Irina Patalakh, Olga Revka, Agata Gołaszewska, Natalia Bielicka, Tomasz Misztal
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引用次数: 0

摘要

本研究旨在确定血小板和活化因子 XIIIa (FXIIIa) 在纤维蛋白网络结构中的作用,并阐明网络压实对血块溶解的影响。 浊度测定法用于单阶段凝血试验,其中 PFP 被视为单因子缺乏血浆(血小板为缺乏因子),而自体 PRP 被视为缺乏因子校正血浆。共聚焦显微镜观察了在静态和流动条件下形成的纤维蛋白网络的结构特征。凝血酶引发的血浆凝固显示出吸收曲线形状的变化,在血小板存在的情况下更为明显。这些变化与凝块成熟过程中纤维蛋白支架的转变有关。在血小板、凝血酶和 Ca2+ 的共同作用下,血浆凝结会经历两个阶段:最初形成血小板-纤维蛋白网络(聚合曲线的第一个峰值),然后在 FXIIIa 的驱动下压实纤维蛋白(第二个峰值),血小板的收缩作用可进一步调节纤维蛋白的压实。这些由血小板和 FXIIIa 介导的结构变化已被证明能决定随后的血块溶解。 血小板聚集体是决定纤维蛋白在血块体积中分布的组织中心。血小板转化的纤维蛋白的镂空结构为其及时裂解提供了必要的先决条件。血小板和 FXIIIa 的相互作用伴随着纤维蛋白凝块的成熟,揭示了这一相互作用的方方面面,可能会为药物治疗与血栓发作和出血倾向相关的疾病提供新的方法。
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Integration of clotting and fibrinolysis: central role of platelets and factor XIIIa.

Purpose: The aim of the present study was to establish the role of platelets and activated factor XIIIa (FXIIIa) in the structuring of the fibrin network as well as to clarify the effect of network compaction on clot lysis.

Methods: Turbidimetry was used for the one-stage clotting test where platelet-free plasma (PFP) is regarded as single factor-deficient plasma (platelets as lacking factor) and autologous platelet-rich plasma (PRP) as deficiency corrected plasma. Structural features of the developed and subsequently lysed fibrin network, formed under static and flow conditions, were visualized by confocal microscopy.

Results: Thrombin-initiated plasma clotting revealed changes in the shape of the absorption curve, more pronounced in the presence of platelets. These changes correlate with the transformation of the fibrin scaffold during clot maturing. With the combined action of platelets, thrombin and Ca2+, plasma clotting passes through two phases: initial formation of a platelet-fibrin network (first peak in the polymerization curve), and then the compaction of fibrin, driven by FXIIIa (the second peak) which can be further modulate by the contractile action of platelets. These structural changes, mediated by platelets and FXIIIa, have been shown to determine subsequent clot lysis.

Conclusions: Platelet aggregates serve as organizing centers that determine the distribution of fibrin in clot volume. The openwork structure of the platelet-transformed fibrin provides the necessary prerequisites for its timely lysis. The revealed aspects of the interaction of platelets and FXIIIa, which accompanies the maturation of a fibrin clot, may lead to new approaches in the pharmacological correction of disorders associated with both thrombotic episodes and bleeding tendency.

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来源期刊
Bioscience Reports
Bioscience Reports 生物-细胞生物学
CiteScore
8.50
自引率
0.00%
发文量
380
审稿时长
6-12 weeks
期刊介绍: Bioscience Reports provides a home for sound scientific research in all areas of cell biology and molecular life sciences. Since 2012, Bioscience Reports has been fully Open Access and publishes all papers under the liberal CC BY licence, giving the life science community quality research to share and discuss.Content before 2012 is subscription-only, and is accessible via archive purchase. Articles are assessed on soundness, providing a home for valid findings and data. We welcome papers that span disciplines (e.g. chemistry, medicine), including papers describing: -new methodologies -tools and reagents to probe biological questions -mechanistic details -disease mechanisms -metabolic processes and their regulation -structure and function -bioenergetics
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