Dmitry V Vishnevetskii, Fedor A Metlin, Yana V Andrianova, Elizaveta E Polyakova, Alexandra I Ivanova, Dmitry V Averkin, Arif R Mekhtiev
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引用次数: 0
摘要
在这项研究中,半胱氨酸银溶胶(CSS)和亚甲基蓝(MB)混合合成了一种新型超分子复合材料 "光凝胶"。粘度测定法、紫外光谱法、动态和电泳光散射法、扫描电子显微镜和能量色散 X 射线光谱法等现代物理化学分析方法的综合应用表明,甲基溴分子主要均匀地分布在 CSS 颗粒形成的凝胶网络的纤维之间的空间。染料分子还通过非共价作用与 CSS 颗粒表面结合。即使在没有光照射的情况下,凝胶对人类鳞状细胞癌也有协同抗癌作用,这就反映了这一事实。在正常角质细胞中观察到了水凝胶的轻微毒性影响。光动力照射明显提高了凝胶的活性,而且仍然存在协同效应。对细胞中自由基氧化的研究表明,凝胶不仅能产生活性氧,而且还有其他作用靶点。通过流式细胞仪分析,我们发现所获得的水凝胶在没有照射和有光照的情况下都会导致细胞周期停滞。从学术和应用科学的角度来看,所获得的凝胶在表皮肿瘤的光动力疗法等方面都具有重大意义。
Preparation of Composite Hydrogels Based on Cysteine-Silver Sol and Methylene Blue as Promising Systems for Anticancer Photodynamic Therapy.
In this study, a novel supramolecular composite, "photogels", was synthesized by mixing of cysteine-silver sol (CSS) and methylene blue (MB). A complex of modern physico-chemical methods of analysis such as viscosimetry, UV spectroscopy, dynamic and electrophoretic light scattering, scanning electron microscopy and energy-dispersive X-ray spectroscopy showed that MB molecules are uniformly localized mainly in the space between fibers of the gel-network formed by CSS particles. Molecules of the dye also bind with the surface of CSS particles by non-covalent interactions. This fact is reflected in the appearance of a synergistic anticancer effect of gels against human squamous cell carcinoma even in the absence of light irradiation. A mild toxic influence of hydrogels was observed in normal keratinocyte cells. Photodynamic exposure significantly increased gel activity, and there remained a synergistic effect. The study of free-radical oxidation in cells has shown that gels are not only capable of generating reactive oxygen species, but also have other targets of action. Flow cytometric analysis allowed us to find out that obtained hydrogels caused cell cycle arrest both without irradiation and with light exposure. The obtained gels are of considerable interest both from the point of view of academics and applied science, for example, in the photodynamic therapy of superficial neoplasms.