增强软骨修复的粘合剂水凝胶技术:概念的初步验证

IF 5 3区 化学 Q1 POLYMER SCIENCE Gels Pub Date : 2024-10-14 DOI:10.3390/gels10100657
Peyman Karami, Robin Martin, Alexis Laurent, Hui Yin Nam, Virginie Philippe, Lee Ann Applegate, Dominique P Pioletti
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引用次数: 0

摘要

膝关节软骨的自然愈合能力有限,使有效治疗方案的制定变得复杂。目前的非细胞疗法(如微骨折)导致修复软骨机械性能差、耐久性低、组织整合不理想。自体软骨细胞植入等先进疗法面临着细胞渗漏和分布不均匀等挑战。成功的细胞疗法有赖于治疗性生物制剂在植入部位的长期存留,但植入材料与周围健康组织的最佳整合仍是一个尚未满足的需求。本研究评估了新开发的光固化粘合剂水凝胶用于软骨修复的效果,重点关注粘合特性、整合性能和支持组织再生的能力。所提出的水凝胶设计具有显著的粘附强度,优于纤维蛋白胶等商业粘合剂。研究人员利用活体山羊模型评估了水凝胶的粘附性能以及与全厚软骨缺损处长达六个月的长期整合。结果表明,无细胞水凝胶处理过的缺损能与周围组织很好地融合,软骨修复能力增强,侧向融合效果显著。体外实验结果进一步证明了水凝胶基质中细胞的高存活率、强大的基质生成和成功的细胞包裹。这些发现凸显了粘合剂水凝胶配方在提高细胞疗法疗效方面的潜力,为膝关节软骨缺损提供了一种潜在的优质疗法。
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An Adhesive Hydrogel Technology for Enhanced Cartilage Repair: A Preliminary Proof of Concept.

Knee cartilage has limited natural healing capacity, complicating the development of effective treatment plans. Current non-cell-based therapies (e.g., microfracture) result in poor repair cartilage mechanical properties, low durability, and suboptimal tissue integration. Advanced treatments, such as autologous chondrocyte implantation, face challenges including cell leakage and inhomogeneous distribution. Successful cell therapy relies on prolonged retention of therapeutic biologicals at the implantation site, yet the optimal integration of implanted material into the surrounding healthy tissue remains an unmet need. This study evaluated the effectiveness of a newly developed photo-curable adhesive hydrogel for cartilage repair, focusing on adhesion properties, integration performance, and ability to support tissue regeneration. The proposed hydrogel design exhibited significant adhesion strength, outperforming commercial adhesives such as fibrin-based glues. An in vivo goat model was used to evaluate the hydrogels' adhesion properties and long-term integration into full-thickness cartilage defects over six months. Results showed that cell-free hydrogel-treated defects achieved superior integration with surrounding tissue and enhanced cartilage repair, with notable lateral integration. In vitro results further demonstrated high cell viability, robust matrix production, and successful cell encapsulation within the hydrogel matrix. These findings highlight the potential of adhesive hydrogel formulations to improve the efficacy of cell-based therapies, offering a potentially superior treatment for knee cartilage defects.

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来源期刊
Gels
Gels POLYMER SCIENCE-
CiteScore
4.70
自引率
19.60%
发文量
707
审稿时长
11 weeks
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