V. S. Orekhov, E. V. Skorobogatov, M. K. Beklemishev
{"title":"利用羰花青 Cy7-Hydrazine 与 4-二甲基氨基苯甲醛的反应识别相似成分的样品并测定氟喹诺酮类药物","authors":"V. S. Orekhov, E. V. Skorobogatov, M. K. Beklemishev","doi":"10.1134/S106193482470093X","DOIUrl":null,"url":null,"abstract":"<p>The work focuses on the development of a fluorimetric version of the fingerprint method based on conducting indicator reactions in the presence of a test sample. Observation of these reactions over time provides a more detailed information compared to batch methods, thereby improving sample recognition and enabling quantitative analysis. The proposed indicator reaction utilizes a commercial carbocyanine dye and 4-dimethylaminobenzaldehyde, whose interaction results in a decrease in fluorescence intensity and changes in absorbance over time. Three fluoroquinolones—moxifloxacin, levofloxacin, and ofloxacin—selectively change the signal in concentrations of 1 μM or higher, while other drugs, including different fluoroquinolones, do not interfere with the determination. Ofloxacin was detected in human urine samples at various times post-drug intake. A potential of using the same indicator reaction for sample recognition was demonstrated on examples of apple juices, soil extracts, and meat of varying freshness. Chemometric methods, including linear discriminant analysis, were used for data processing. The method achieved 97% accuracy in discriminating fifteen apple juice samples, 94% accuracy for ten apple juices from 2022 and 2023, 99% accuracy for ten soil samples, and successfully determined the freshness of five meat samples.</p>","PeriodicalId":606,"journal":{"name":"Journal of Analytical Chemistry","volume":null,"pages":null},"PeriodicalIF":1.0000,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Recognition of Samples of Similar Composition and Determination of Fluoroquinolones Using the Reaction of Carbocyanine Cy7-Hydrazine with 4-Dimethylaminobenzaldehyde\",\"authors\":\"V. S. Orekhov, E. V. Skorobogatov, M. K. Beklemishev\",\"doi\":\"10.1134/S106193482470093X\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>The work focuses on the development of a fluorimetric version of the fingerprint method based on conducting indicator reactions in the presence of a test sample. Observation of these reactions over time provides a more detailed information compared to batch methods, thereby improving sample recognition and enabling quantitative analysis. The proposed indicator reaction utilizes a commercial carbocyanine dye and 4-dimethylaminobenzaldehyde, whose interaction results in a decrease in fluorescence intensity and changes in absorbance over time. Three fluoroquinolones—moxifloxacin, levofloxacin, and ofloxacin—selectively change the signal in concentrations of 1 μM or higher, while other drugs, including different fluoroquinolones, do not interfere with the determination. Ofloxacin was detected in human urine samples at various times post-drug intake. A potential of using the same indicator reaction for sample recognition was demonstrated on examples of apple juices, soil extracts, and meat of varying freshness. Chemometric methods, including linear discriminant analysis, were used for data processing. The method achieved 97% accuracy in discriminating fifteen apple juice samples, 94% accuracy for ten apple juices from 2022 and 2023, 99% accuracy for ten soil samples, and successfully determined the freshness of five meat samples.</p>\",\"PeriodicalId\":606,\"journal\":{\"name\":\"Journal of Analytical Chemistry\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.0000,\"publicationDate\":\"2024-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Analytical Chemistry\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://link.springer.com/article/10.1134/S106193482470093X\",\"RegionNum\":4,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Analytical Chemistry","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1134/S106193482470093X","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
Recognition of Samples of Similar Composition and Determination of Fluoroquinolones Using the Reaction of Carbocyanine Cy7-Hydrazine with 4-Dimethylaminobenzaldehyde
The work focuses on the development of a fluorimetric version of the fingerprint method based on conducting indicator reactions in the presence of a test sample. Observation of these reactions over time provides a more detailed information compared to batch methods, thereby improving sample recognition and enabling quantitative analysis. The proposed indicator reaction utilizes a commercial carbocyanine dye and 4-dimethylaminobenzaldehyde, whose interaction results in a decrease in fluorescence intensity and changes in absorbance over time. Three fluoroquinolones—moxifloxacin, levofloxacin, and ofloxacin—selectively change the signal in concentrations of 1 μM or higher, while other drugs, including different fluoroquinolones, do not interfere with the determination. Ofloxacin was detected in human urine samples at various times post-drug intake. A potential of using the same indicator reaction for sample recognition was demonstrated on examples of apple juices, soil extracts, and meat of varying freshness. Chemometric methods, including linear discriminant analysis, were used for data processing. The method achieved 97% accuracy in discriminating fifteen apple juice samples, 94% accuracy for ten apple juices from 2022 and 2023, 99% accuracy for ten soil samples, and successfully determined the freshness of five meat samples.
期刊介绍:
The Journal of Analytical Chemistry is an international peer reviewed journal that covers theoretical and applied aspects of analytical chemistry; it informs the reader about new achievements in analytical methods, instruments and reagents. Ample space is devoted to problems arising in the analysis of vital media such as water and air. Consideration is given to the detection and determination of metal ions, anions, and various organic substances. The journal welcomes manuscripts from all countries in the English or Russian language.