利用羰花青 Cy7-Hydrazine 与 4-二甲基氨基苯甲醛的反应识别相似成分的样品并测定氟喹诺酮类药物

IF 1 4区 化学 Q4 CHEMISTRY, ANALYTICAL Journal of Analytical Chemistry Pub Date : 2024-11-01 DOI:10.1134/S106193482470093X
V. S. Orekhov, E. V. Skorobogatov, M. K. Beklemishev
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引用次数: 0

摘要

这项工作的重点是开发一种荧光指纹法,其基础是在测试样品存在的情况下进行指示剂反应。与批量方法相比,通过观察这些反应的时间变化,可以获得更详细的信息,从而提高样品识别率,实现定量分析。拟议的指示剂反应利用一种商用羰花青染料和 4-二甲氨基苯甲醛,它们的相互作用会导致荧光强度下降,吸光度随时间发生变化。三种氟喹诺酮类药物--氧氟沙星、左氧氟沙星和氧氟沙星--会选择性地改变 1 μM 或更高浓度的信号,而其他药物,包括不同的氟喹诺酮类药物,不会干扰测定。在服药后不同时间段的人体尿样中都检测到了氧氟沙星。在苹果汁、土壤提取物和不同新鲜度的肉类样品中,证明了使用相同的指示剂反应进行样品识别的可能性。数据处理采用了化学计量学方法,包括线性判别分析。该方法鉴别 15 个苹果汁样品的准确率达到 97%,鉴别 10 个 2022 年和 2023 年苹果汁样品的准确率达到 94%,鉴别 10 个土壤样品的准确率达到 99%,并成功鉴别了 5 个肉类样品的新鲜度。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Recognition of Samples of Similar Composition and Determination of Fluoroquinolones Using the Reaction of Carbocyanine Cy7-Hydrazine with 4-Dimethylaminobenzaldehyde

The work focuses on the development of a fluorimetric version of the fingerprint method based on conducting indicator reactions in the presence of a test sample. Observation of these reactions over time provides a more detailed information compared to batch methods, thereby improving sample recognition and enabling quantitative analysis. The proposed indicator reaction utilizes a commercial carbocyanine dye and 4-dimethylaminobenzaldehyde, whose interaction results in a decrease in fluorescence intensity and changes in absorbance over time. Three fluoroquinolones—moxifloxacin, levofloxacin, and ofloxacin—selectively change the signal in concentrations of 1 μM or higher, while other drugs, including different fluoroquinolones, do not interfere with the determination. Ofloxacin was detected in human urine samples at various times post-drug intake. A potential of using the same indicator reaction for sample recognition was demonstrated on examples of apple juices, soil extracts, and meat of varying freshness. Chemometric methods, including linear discriminant analysis, were used for data processing. The method achieved 97% accuracy in discriminating fifteen apple juice samples, 94% accuracy for ten apple juices from 2022 and 2023, 99% accuracy for ten soil samples, and successfully determined the freshness of five meat samples.

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来源期刊
Journal of Analytical Chemistry
Journal of Analytical Chemistry 化学-分析化学
CiteScore
2.10
自引率
9.10%
发文量
146
审稿时长
13 months
期刊介绍: The Journal of Analytical Chemistry is an international peer reviewed journal that covers theoretical and applied aspects of analytical chemistry; it informs the reader about new achievements in analytical methods, instruments and reagents. Ample space is devoted to problems arising in the analysis of vital media such as water and air. Consideration is given to the detection and determination of metal ions, anions, and various organic substances. The journal welcomes manuscripts from all countries in the English or Russian language.
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