利用枪式蛋白质组学对加热大鼠晶状体中的蛋白质表达进行半定量分析。

IF 3.4 3区 医学 Q2 MEDICINE, RESEARCH & EXPERIMENTAL Molecular medicine reports Pub Date : 2025-01-01 Epub Date: 2024-11-14 DOI:10.3892/mmr.2024.13391
Hiroko Otake, Shuya Masuda, Tetsushi Yamamoto, Yoshiki Miyata, Yosuke Nakazawa, Naoki Yamamoto, Atsushi Taga, Hiroshi Sasaki, Noriaki Nagai
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引用次数: 0

摘要

以往的研究表明,晶状体中估计的累积热暴露与核性白内障的发病率之间存在很强的相关性;然而,温度与白内障之间的确切关系仍有待全面阐明。本研究采用基于枪式液相色谱/质谱的全局蛋白质组学方法,研究了在正常温度(35.0˚C)和稍高温度(37.5˚C)条件下培养的晶状体中诱发白内障的因素。在大鼠晶状体中,共鉴定出 190 种蛋白质。其中,在 35.0˚C 和 37.5˚C 条件下培养的晶状体中发现了 48 种蛋白质(25.3%)。此外,有 85 个蛋白质(44.7%)是在 35.0˚C 条件下培养的晶状体所特有的,而有 57 个蛋白质(30.0%)是在 37.5˚C 条件下培养的晶状体所特有的。使用光谱计数和基因本体分析的无标记半定量方法检测了在 37.5˚C 培养的大鼠晶状体中蛋白质表达的变化。丝蛋白和波形蛋白是维持晶状体结构的关键因素,它们的表达量有所下降。这些发现可作为阐明温度与核性白内障发病之间关系的重要指标。
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Semiquantitative analysis of protein expression in heated rat lens using shotgun proteomics.

Previous studies have reported that a strong correlation between the estimated cumulative thermal exposure in the crystalline lens and the incidence of nuclear cataracts; however, the precise relationship between temperature and cataracts remains to be fully elucidated. In the present study, the shotgun liquid chromatography/mass spectroscopy‑based global proteomic approach was applied to investigate cataract‑inducing factors in lens cultured at normal (35.0˚C) and slightly warmer (37.5˚C) conditions. In the rat lens, 190 proteins (total) were identified. Of these, 48 proteins (25.3%) were found in lenses cultured at both 35.0˚C and 37.5˚C. Moreover, 85 proteins (44.7%) were unique to lenses cultured at 35.0˚C, while 57 proteins (30.0%) were unique to lenses cultured at 37.5˚C. Protein expression changes in rat lenses cultured at 37.5˚C were examined using a label‑free semiquantitative approach that uses spectral counting and Gene Ontology analysis. Filensin and vimentin protein expression, key factors in maintaining lens structure, were decreased. These findings may serve as a valuable indicator for elucidating the relationship between temperature and the onset of nuclear cataracts.

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来源期刊
Molecular medicine reports
Molecular medicine reports 医学-病理学
CiteScore
7.60
自引率
0.00%
发文量
321
审稿时长
1.5 months
期刊介绍: Molecular Medicine Reports is a monthly, peer-reviewed journal available in print and online, that includes studies devoted to molecular medicine, underscoring aspects including pharmacology, pathology, genetics, neurosciences, infectious diseases, molecular cardiology and molecular surgery. In vitro and in vivo studies of experimental model systems pertaining to the mechanisms of a variety of diseases offer researchers the necessary tools and knowledge with which to aid the diagnosis and treatment of human diseases.
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