Renchi Fang , Lingfang Di , Xuebin Tian , Yongfeng Bai
{"title":"一株产NDM-1的ST152肺炎克雷伯菌的基因组和系统发育分析","authors":"Renchi Fang , Lingfang Di , Xuebin Tian , Yongfeng Bai","doi":"10.1016/j.jgar.2024.11.005","DOIUrl":null,"url":null,"abstract":"<div><h3>Purpose</h3><div>The aim of this study was to characterize the genomic structure of the first isolated ST152 <em>Klebsiella pneumoniae</em> strain carrying <em>bla</em><sub>NDM-1</sub> in China.</div></div><div><h3>Methods</h3><div>Antimicrobial susceptibility was determined by broth microdilution method. Whole-genome sequencing was conducted using the Illumina HiSeq and Oxford Nanopore GridION X5 platforms, and the genomic features were analyzed using a range of bioinformatics tools.</div></div><div><h3>Results</h3><div>The strain HZKP1 demonstrated resistance to cefepime, ceftazidime, amoxicillin-clavulanic acid, amikacin, ciprofloxacin, meropenem, imipenem, and ertapenem, while showing sensitivity to tigecycline and colistin. It was classified as sequence type (ST) 152, K locus (KL) 105 and OC locus (OCL) O3b, respectively. The <em>bla</em><sub>NDM-1</sub> gene was identified on the IncX3 plasmid pHZKP1-NDM (54,035 bp), located downstream of the IS<em>Kox3</em>-IS<em>3000</em>-IS<em>Aba125</em>-IS<em>5</em> segment and upstream of the <em>ble</em><sub>MBL</sub>-IS<em>26</em>-<em>bla</em><sub>SHV-69</sub>-IS<em>26</em> segment. Phylogenetic analysis indicated its closest relative to be a strain isolated from a wound swab sample in Tanzania in 2013, differing by 598 alleles.</div></div><div><h3>Conclusion</h3><div>We characterized genomic features of the first ST152 <em>K. pneumoniae</em> strain isolated in China. Our findings provide insights into the current status of resistance in new lineages within the region and enhance comprehension of <em>K. pneumoniae</em> carbapenem resistance transmission from a genetic structure perspective.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"40 ","pages":"Pages 34-36"},"PeriodicalIF":3.7000,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Genomic and phylogenetic analysis of a NDM-1 producing ST152 Klebsiella pneumoniae isolated from a bloodstream infection in China\",\"authors\":\"Renchi Fang , Lingfang Di , Xuebin Tian , Yongfeng Bai\",\"doi\":\"10.1016/j.jgar.2024.11.005\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Purpose</h3><div>The aim of this study was to characterize the genomic structure of the first isolated ST152 <em>Klebsiella pneumoniae</em> strain carrying <em>bla</em><sub>NDM-1</sub> in China.</div></div><div><h3>Methods</h3><div>Antimicrobial susceptibility was determined by broth microdilution method. Whole-genome sequencing was conducted using the Illumina HiSeq and Oxford Nanopore GridION X5 platforms, and the genomic features were analyzed using a range of bioinformatics tools.</div></div><div><h3>Results</h3><div>The strain HZKP1 demonstrated resistance to cefepime, ceftazidime, amoxicillin-clavulanic acid, amikacin, ciprofloxacin, meropenem, imipenem, and ertapenem, while showing sensitivity to tigecycline and colistin. It was classified as sequence type (ST) 152, K locus (KL) 105 and OC locus (OCL) O3b, respectively. The <em>bla</em><sub>NDM-1</sub> gene was identified on the IncX3 plasmid pHZKP1-NDM (54,035 bp), located downstream of the IS<em>Kox3</em>-IS<em>3000</em>-IS<em>Aba125</em>-IS<em>5</em> segment and upstream of the <em>ble</em><sub>MBL</sub>-IS<em>26</em>-<em>bla</em><sub>SHV-69</sub>-IS<em>26</em> segment. Phylogenetic analysis indicated its closest relative to be a strain isolated from a wound swab sample in Tanzania in 2013, differing by 598 alleles.</div></div><div><h3>Conclusion</h3><div>We characterized genomic features of the first ST152 <em>K. pneumoniae</em> strain isolated in China. Our findings provide insights into the current status of resistance in new lineages within the region and enhance comprehension of <em>K. pneumoniae</em> carbapenem resistance transmission from a genetic structure perspective.</div></div>\",\"PeriodicalId\":15936,\"journal\":{\"name\":\"Journal of global antimicrobial resistance\",\"volume\":\"40 \",\"pages\":\"Pages 34-36\"},\"PeriodicalIF\":3.7000,\"publicationDate\":\"2025-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of global antimicrobial resistance\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2213716524004466\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of global antimicrobial resistance","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2213716524004466","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
Genomic and phylogenetic analysis of a NDM-1 producing ST152 Klebsiella pneumoniae isolated from a bloodstream infection in China
Purpose
The aim of this study was to characterize the genomic structure of the first isolated ST152 Klebsiella pneumoniae strain carrying blaNDM-1 in China.
Methods
Antimicrobial susceptibility was determined by broth microdilution method. Whole-genome sequencing was conducted using the Illumina HiSeq and Oxford Nanopore GridION X5 platforms, and the genomic features were analyzed using a range of bioinformatics tools.
Results
The strain HZKP1 demonstrated resistance to cefepime, ceftazidime, amoxicillin-clavulanic acid, amikacin, ciprofloxacin, meropenem, imipenem, and ertapenem, while showing sensitivity to tigecycline and colistin. It was classified as sequence type (ST) 152, K locus (KL) 105 and OC locus (OCL) O3b, respectively. The blaNDM-1 gene was identified on the IncX3 plasmid pHZKP1-NDM (54,035 bp), located downstream of the ISKox3-IS3000-ISAba125-IS5 segment and upstream of the bleMBL-IS26-blaSHV-69-IS26 segment. Phylogenetic analysis indicated its closest relative to be a strain isolated from a wound swab sample in Tanzania in 2013, differing by 598 alleles.
Conclusion
We characterized genomic features of the first ST152 K. pneumoniae strain isolated in China. Our findings provide insights into the current status of resistance in new lineages within the region and enhance comprehension of K. pneumoniae carbapenem resistance transmission from a genetic structure perspective.
期刊介绍：
The Journal of Global Antimicrobial Resistance (JGAR) is a quarterly online journal run by an international Editorial Board that focuses on the global spread of antibiotic-resistant microbes.
JGAR is a dedicated journal for all professionals working in research, health care, the environment and animal infection control, aiming to track the resistance threat worldwide and provides a single voice devoted to antimicrobial resistance (AMR).
Featuring peer-reviewed and up to date research articles, reviews, short notes and hot topics JGAR covers the key topics related to antibacterial, antiviral, antifungal and antiparasitic resistance.
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