应用大鼠腹主动脉移植模型评价聚ε-己内酯纳米纤维片血管的通畅率和内皮化。

IF 1.6 4区 医学 Q2 SURGERY Frontiers in Surgery Pub Date : 2024-11-21 eCollection Date: 2024-01-01 DOI:10.3389/fsurg.2024.1464155
Yuta Kikuchi, Naohiro Wakabayashi, Daikelly I Braghirolli, Patricia Pranke, Hiroyuki Kamiya, Kyohei Oyama
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引用次数: 0

摘要

导言:全球心血管疾病的增加导致人工小口径血管移植物用于搭桥手术,如冠状动脉和远端动脉搭桥手术的发展。然而,对于创建血管移植物的最佳方法尚无共识。聚己内酯(PCL)是一种生物相容性和可生物降解的材料,已被广泛研究作为组织再生的支架,包括血管移植。本研究采用PCL纳米纤维片(PCL graft)制备血管移植物,并采用大鼠腹主动脉移植模型对其性能进行了研究。方法:采用静电纺丝法制备PCL纳米纤维片。这些纳米纤维片被卷起。用PCL溶液在层与层之间涂上胶水,制成内径为1mm的PCL纳米纤维血管移植物。将长度为7mm的PCL移植物植入大鼠腹主动脉。随后,采用移植物吻合口远端主动脉半切除部位脉动血流量测定通畅程度,采用苏木精、伊红及免疫荧光染色法检测内皮化情况。结果:PCL移植物2周通畅率为57.1%(21例中12例),作为小口径血管移植物,其通畅率尚不理想。然而,专利病例显示cd31阳性内皮细胞层在内腔和自体细胞浸润到支架中,表明自体血管样再生。相比之下,闭塞的情况下,纳米纤维层被破坏;内层折叠到管腔的中间。这一观察结果表明,PCL移植物内层的解体扰乱了血液流动并引发了闭塞。结论:PCL移植具有自体血管样再生;然而,关于通畅性,由卷曲的PCL纳米纤维片制成的移植物在结构上存在弱点。为了实现PCL移植的长期和高通畅率,需要进一步的改进。
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Evaluation of the patency rate and endothelialization of a poly-ε-caprolactone, nanofiber sheet-based vascular graft using a rat abdominal aortic implantation model.

Introduction: The global increase in cardiovascular diseases has resulted in an augmented development of artificial small-caliber vascular grafts used in bypass graft surgeries, such as coronary and distal artery bypass graft surgeries. However, no consensus exists regarding the best method for creating vascular grafts. Poly-ε-caprolactone (PCL) is a biocompatible and biodegradable material that has been widely studied as a scaffold for tissue regeneration, inclusive of vascular grafts. In this study, a vascular graft was created from a PCL nanofiber sheet (PCL graft), and the performance thereof was examined using a rat abdominal aortic implantation model.

Methods: The PCL nanofiber sheets were created using an electrospinning machine. These nanofiber sheets were rolled up. Glue was applied between layers using a PCL solution to create a PCL nanofiber vascular graft, with an inner diameter of 1 mm. PCL grafts with 7 mm length were implanted into the abdominal aorta of rats. Thereafter, the patency was determined by pulsating blood flow from the hemiresection site of the distal aorta of the graft anastomosis, and endothelialization was examined using hematoxylin and eosin and immunofluorescent staining methods.

Results: The patency rate of the PCL graft at 2 weeks was 57.1% (12 of 21 cases), which is not satisfactory as a small-caliber vascular graft. Patent cases, however, revealed a CD31-positive endothelial cell layer in the inner lumen and autologous cell infiltration into the scaffold, indicating autologous vessel-like regeneration. By contrast, the occluded cases showed disassembly of the nanofiber layers; and the inner layers folded into the middle of the lumen. This observation suggested that the disassembled inner layer of the PCL graft disturbed the blood flow and triggered occlusion.

Conclusions: PCL grafts can exhibit autologous vessel-like regeneration; nonetheless, regarding patency, grafts made from rolled-up PCL nanofiber sheets have structural weaknesses. Further improvements are required to achieve a long-term and high patency rate for PCL grafts.

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来源期刊
Frontiers in Surgery
Frontiers in Surgery Medicine-Surgery
CiteScore
1.90
自引率
11.10%
发文量
1872
审稿时长
12 weeks
期刊介绍: Evidence of surgical interventions go back to prehistoric times. Since then, the field of surgery has developed into a complex array of specialties and procedures, particularly with the advent of microsurgery, lasers and minimally invasive techniques. The advanced skills now required from surgeons has led to ever increasing specialization, though these still share important fundamental principles. Frontiers in Surgery is the umbrella journal representing the publication interests of all surgical specialties. It is divided into several “Specialty Sections” listed below. All these sections have their own Specialty Chief Editor, Editorial Board and homepage, but all articles carry the citation Frontiers in Surgery. Frontiers in Surgery calls upon medical professionals and scientists from all surgical specialties to publish their experimental and clinical studies in this journal. By assembling all surgical specialties, which nonetheless retain their independence, under the common umbrella of Frontiers in Surgery, a powerful publication venue is created. Since there is often overlap and common ground between the different surgical specialties, assembly of all surgical disciplines into a single journal will foster a collaborative dialogue amongst the surgical community. This means that publications, which are also of interest to other surgical specialties, will reach a wider audience and have greater impact. The aim of this multidisciplinary journal is to create a discussion and knowledge platform of advances and research findings in surgical practice today to continuously improve clinical management of patients and foster innovation in this field.
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