{"title":"利用铁交联海藻酸凝胶为土壤微生物形成生物膜提供新型支架。","authors":"Ikuko Machida-Sano, Hiroshi Koizumi, Shinpei Yoshitake","doi":"10.1093/bbb/zbae197","DOIUrl":null,"url":null,"abstract":"<p><p>This study aimed to evaluate the suitability of alginate gels, specifically ferric-ion-cross-linked alginate (Fe-alginate) and calcium-ion-cross-linked alginate (Ca-alginate), as scaffolds for soil microbial attachment and biofilm formation in soil. Staining with crystal violet and observations with scanning electron microscopy showed that microorganisms formed biofilms on Fe-alginate surfaces in the soil. When the soil was incubated with Fe-alginate, microbial biomass, estimated by adenosine triphosphate content, increased not only in the Fe-alginate but also in the surrounding soil. The weight of Ca-alginate in the soil decreased with time owing to chemical dissolution. However, the weight of Fe-alginate in the soil did not decrease, likely because it was protected by the microbial biofilm that formed on its surface. These results demonstrate that the use of Fe-alginate, in contrast to Ca-alginate, as a scaffold may allow for more efficient use of soil microbial functions in agriculture and bioremediation.</p>","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":""},"PeriodicalIF":1.4000,"publicationDate":"2024-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A novel scaffold for biofilm formation by soil microbes using iron-cross-linked alginate gels.\",\"authors\":\"Ikuko Machida-Sano, Hiroshi Koizumi, Shinpei Yoshitake\",\"doi\":\"10.1093/bbb/zbae197\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>This study aimed to evaluate the suitability of alginate gels, specifically ferric-ion-cross-linked alginate (Fe-alginate) and calcium-ion-cross-linked alginate (Ca-alginate), as scaffolds for soil microbial attachment and biofilm formation in soil. Staining with crystal violet and observations with scanning electron microscopy showed that microorganisms formed biofilms on Fe-alginate surfaces in the soil. When the soil was incubated with Fe-alginate, microbial biomass, estimated by adenosine triphosphate content, increased not only in the Fe-alginate but also in the surrounding soil. The weight of Ca-alginate in the soil decreased with time owing to chemical dissolution. However, the weight of Fe-alginate in the soil did not decrease, likely because it was protected by the microbial biofilm that formed on its surface. These results demonstrate that the use of Fe-alginate, in contrast to Ca-alginate, as a scaffold may allow for more efficient use of soil microbial functions in agriculture and bioremediation.</p>\",\"PeriodicalId\":9175,\"journal\":{\"name\":\"Bioscience, Biotechnology, and Biochemistry\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":1.4000,\"publicationDate\":\"2024-12-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bioscience, Biotechnology, and Biochemistry\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1093/bbb/zbae197\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioscience, Biotechnology, and Biochemistry","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1093/bbb/zbae197","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
A novel scaffold for biofilm formation by soil microbes using iron-cross-linked alginate gels.
This study aimed to evaluate the suitability of alginate gels, specifically ferric-ion-cross-linked alginate (Fe-alginate) and calcium-ion-cross-linked alginate (Ca-alginate), as scaffolds for soil microbial attachment and biofilm formation in soil. Staining with crystal violet and observations with scanning electron microscopy showed that microorganisms formed biofilms on Fe-alginate surfaces in the soil. When the soil was incubated with Fe-alginate, microbial biomass, estimated by adenosine triphosphate content, increased not only in the Fe-alginate but also in the surrounding soil. The weight of Ca-alginate in the soil decreased with time owing to chemical dissolution. However, the weight of Fe-alginate in the soil did not decrease, likely because it was protected by the microbial biofilm that formed on its surface. These results demonstrate that the use of Fe-alginate, in contrast to Ca-alginate, as a scaffold may allow for more efficient use of soil microbial functions in agriculture and bioremediation.
期刊介绍:
Bioscience, Biotechnology, and Biochemistry publishes high-quality papers providing chemical and biological analyses of vital phenomena exhibited by animals, plants, and microorganisms, the chemical structures and functions of their products, and related matters. The Journal plays a major role in communicating to a global audience outstanding basic and applied research in all fields subsumed by the Japan Society for Bioscience, Biotechnology, and Agrochemistry (JSBBA).