Xiaoqian Long , Feng Lin , Biao Tang , Fengcheng Miao , Zhiyu Li , Yao Shen , Hua Yang , Jiangang Ma
{"title":"从屠宰场废弃物中分离出的带有 tet（X6）和 blaNDM-1 的靛蓝不动杆菌。","authors":"Xiaoqian Long , Feng Lin , Biao Tang , Fengcheng Miao , Zhiyu Li , Yao Shen , Hua Yang , Jiangang Ma","doi":"10.1016/j.jgar.2024.12.004","DOIUrl":null,"url":null,"abstract":"<div><h3>Objectives</h3><div><em>Acinetobacter indicus</em> is an important pathogen of nosocomial infection. The purpose of this study was to analyze the resistance and transmission of <em>A. indicus</em> strain AIBD14 isolated from slaughterhouse environment.</div></div><div><h3>Methods</h3><div>A total of 96 environmental samples were collected from slaughterhouse. The antimicrobial susceptibility test was carried out by microbroth dilution method and <em>E</em>-test. Whole genome sequencing and bioinformatics analysis of the AIBD14 were performed, then S1-PFGE and southern blot verified the location of <em>bla</em><sub>NDM-1</sub> and <em>tet</em>(X6).</div></div><div><h3>Results</h3><div>The AIBD14 is resistant to meropenem but susceptibility to tigecycline, and coharboring <em>bla</em><sub>NDM-1</sub> and <em>tet</em>(X6). The <em>bla</em><sub>NDM-1</sub> is located on the pAIBD14-NDM-1 that cannot be transferred by conjugation. Specifically, <em>bla</em><sub>NDM-1</sub> is located on the transposon Tn<em>125</em>, and <em>bla</em><sub>NDM-1</sub> can be transferred to other species with the help of transposon. The genetic background of <em>bla</em><sub>NDM-1</sub> is \"IS<em>Aba125</em>-<em>bla</em><sub>NDM-1</sub>-<em>ble</em><sub>MEL</sub>-<em>dsbD</em>-<em>cutA</em>-<em>groES</em>-<em>groEL</em>-<em>insE</em>-IS<em>Aba125</em>\". pAIBD14-NDM-1 is classified into the GR31 plasmid based on the homology of the <em>repB</em>. Meanwhile, there are two XerC/D-like binding sites on the plasmid, which can mediate the transfer of resistance genes. The <em>tet</em>(X6) gene is located on the chromosome of AIBD14, its downstream is accompanied by the neglected macrolide resistance gene <em>estT</em>, and there is a single copy of the insertion element IS<em>CR2</em> around <em>tet</em>(X6) as the genetic background \"IS<em>Aba4</em>-IS<em>3</em>-hp-hp-<em>tet</em>(X6)-<em>estT</em>-<em>guaA</em>-IS<em>CR2</em>\".</div></div><div><h3>Conclusions</h3><div>This is the first report of the coexistence of <em>tet</em>(X6) and <em>bla</em><sub>NDM-1</sub> in the <em>A. indicus</em>, and it has the risk of horizontal transfer across multiple species. So strict monitoring the multiple-resistant bacteria in the industrial chain is necessary based on the \"One Heath\".</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"41 ","pages":"Pages 1-7"},"PeriodicalIF":3.7000,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Acinetobacter indicus Coharboring Tet(X6) and blaNDM-1 Isolated From Slaughterhouse Waste\",\"authors\":\"Xiaoqian Long , Feng Lin , Biao Tang , Fengcheng Miao , Zhiyu Li , Yao Shen , Hua Yang , Jiangang Ma\",\"doi\":\"10.1016/j.jgar.2024.12.004\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Objectives</h3><div><em>Acinetobacter indicus</em> is an important pathogen of nosocomial infection. The purpose of this study was to analyze the resistance and transmission of <em>A. indicus</em> strain AIBD14 isolated from slaughterhouse environment.</div></div><div><h3>Methods</h3><div>A total of 96 environmental samples were collected from slaughterhouse. The antimicrobial susceptibility test was carried out by microbroth dilution method and <em>E</em>-test. Whole genome sequencing and bioinformatics analysis of the AIBD14 were performed, then S1-PFGE and southern blot verified the location of <em>bla</em><sub>NDM-1</sub> and <em>tet</em>(X6).</div></div><div><h3>Results</h3><div>The AIBD14 is resistant to meropenem but susceptibility to tigecycline, and coharboring <em>bla</em><sub>NDM-1</sub> and <em>tet</em>(X6). The <em>bla</em><sub>NDM-1</sub> is located on the pAIBD14-NDM-1 that cannot be transferred by conjugation. Specifically, <em>bla</em><sub>NDM-1</sub> is located on the transposon Tn<em>125</em>, and <em>bla</em><sub>NDM-1</sub> can be transferred to other species with the help of transposon. The genetic background of <em>bla</em><sub>NDM-1</sub> is \\\"IS<em>Aba125</em>-<em>bla</em><sub>NDM-1</sub>-<em>ble</em><sub>MEL</sub>-<em>dsbD</em>-<em>cutA</em>-<em>groES</em>-<em>groEL</em>-<em>insE</em>-IS<em>Aba125</em>\\\". pAIBD14-NDM-1 is classified into the GR31 plasmid based on the homology of the <em>repB</em>. Meanwhile, there are two XerC/D-like binding sites on the plasmid, which can mediate the transfer of resistance genes. The <em>tet</em>(X6) gene is located on the chromosome of AIBD14, its downstream is accompanied by the neglected macrolide resistance gene <em>estT</em>, and there is a single copy of the insertion element IS<em>CR2</em> around <em>tet</em>(X6) as the genetic background \\\"IS<em>Aba4</em>-IS<em>3</em>-hp-hp-<em>tet</em>(X6)-<em>estT</em>-<em>guaA</em>-IS<em>CR2</em>\\\".</div></div><div><h3>Conclusions</h3><div>This is the first report of the coexistence of <em>tet</em>(X6) and <em>bla</em><sub>NDM-1</sub> in the <em>A. indicus</em>, and it has the risk of horizontal transfer across multiple species. So strict monitoring the multiple-resistant bacteria in the industrial chain is necessary based on the \\\"One Heath\\\".</div></div>\",\"PeriodicalId\":15936,\"journal\":{\"name\":\"Journal of global antimicrobial resistance\",\"volume\":\"41 \",\"pages\":\"Pages 1-7\"},\"PeriodicalIF\":3.7000,\"publicationDate\":\"2024-12-17\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of global antimicrobial resistance\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2213716524004636\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of global antimicrobial resistance","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2213716524004636","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
Acinetobacter indicus Coharboring Tet(X6) and blaNDM-1 Isolated From Slaughterhouse Waste
Objectives
Acinetobacter indicus is an important pathogen of nosocomial infection. The purpose of this study was to analyze the resistance and transmission of A. indicus strain AIBD14 isolated from slaughterhouse environment.
Methods
A total of 96 environmental samples were collected from slaughterhouse. The antimicrobial susceptibility test was carried out by microbroth dilution method and E-test. Whole genome sequencing and bioinformatics analysis of the AIBD14 were performed, then S1-PFGE and southern blot verified the location of blaNDM-1 and tet(X6).
Results
The AIBD14 is resistant to meropenem but susceptibility to tigecycline, and coharboring blaNDM-1 and tet(X6). The blaNDM-1 is located on the pAIBD14-NDM-1 that cannot be transferred by conjugation. Specifically, blaNDM-1 is located on the transposon Tn125, and blaNDM-1 can be transferred to other species with the help of transposon. The genetic background of blaNDM-1 is "ISAba125-blaNDM-1-bleMEL-dsbD-cutA-groES-groEL-insE-ISAba125". pAIBD14-NDM-1 is classified into the GR31 plasmid based on the homology of the repB. Meanwhile, there are two XerC/D-like binding sites on the plasmid, which can mediate the transfer of resistance genes. The tet(X6) gene is located on the chromosome of AIBD14, its downstream is accompanied by the neglected macrolide resistance gene estT, and there is a single copy of the insertion element ISCR2 around tet(X6) as the genetic background "ISAba4-IS3-hp-hp-tet(X6)-estT-guaA-ISCR2".
Conclusions
This is the first report of the coexistence of tet(X6) and blaNDM-1 in the A. indicus, and it has the risk of horizontal transfer across multiple species. So strict monitoring the multiple-resistant bacteria in the industrial chain is necessary based on the "One Heath".
期刊介绍：
The Journal of Global Antimicrobial Resistance (JGAR) is a quarterly online journal run by an international Editorial Board that focuses on the global spread of antibiotic-resistant microbes.
JGAR is a dedicated journal for all professionals working in research, health care, the environment and animal infection control, aiming to track the resistance threat worldwide and provides a single voice devoted to antimicrobial resistance (AMR).
Featuring peer-reviewed and up to date research articles, reviews, short notes and hot topics JGAR covers the key topics related to antibacterial, antiviral, antifungal and antiparasitic resistance.
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