一种具有防污抗炎活性的新型HEMA共聚物水凝胶,可作为一种有前景的医疗器械防微生物粘附涂层。

IF 2.6 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biofouling Pub Date : 2024-12-22 DOI:10.1080/08927014.2024.2442011
Hadeel Abu Mahfouz, Ola Tarawneh, Lama Hamadneh, Muayad Esaifan, Sameer Al-Kouz, Ala A Alhusban, Mohammad Abu-Sini, Lana Hamdan, Buthaina Hussein, Mohammad Hailat
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引用次数: 0

摘要

与抗菌剂相比,抗粘附表面可以减少导管中细菌的粘附和生物膜的形成,提供更好的选择性,效率和设备寿命。在这项研究中,创造并测试了新型阴离子表面生物材料,以减少微生物在医疗器械涂层中的粘附和定植。顺丁烯二酸酐(MA)与2-HEMA以不同的量聚合生成p(HEMA-co-MA)水凝胶共聚物。傅里叶变换红外表征(ATR-FTIR)、热分析、扫描电子显微镜与能量色散x射线光谱、溶胀能力、细胞毒性评估和混合生物膜形成能力用于表征共聚物水凝胶。根据ISO和ASTM标准的指导和规定对水凝胶进行了评价。该聚合物致密,两种单体之间具有稳定的交联,对人胚胎肾(HEK) 293细胞系无毒,并显著减少细菌生物膜的形成。此外,增加MA的量会影响TGF-1基因的表达,其中基因表达显著升高,特别是在MA的最高百分比时。此外,聚合物中高比例的MA改善了新聚合物的热性能、薄膜柔韧性和膨胀能力。这些新型聚合物有望成为改善导管生物材料性能和修饰指定设备表面以减少微生物感染和生长的材料。
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A novel HEMA copolymer hydrogel with antifouling and anti-inflammatory activity as a promising medical device coating layer to prevent microbial adhesion.

Compared to antimicrobial agents, anti-adhesive surfaces can reduce bacteria adhesion and biofilm formation in catheters, providing better selectivity, efficiency, and device life span. In this research, novel anionic surface biomaterials were created and tested to reduce microbial adhesion and colonization in medical device coating. Maleic anhydride (MA) was polymerized with 2-HEMA in varying amounts to produce a p(HEMA-co-MA) hydrogel copolymer. Fourier transforms infrared characterization (ATR-FTIR), thermal analysis, scanning electron microscopy with energy-dispersive X-ray spectroscopy, swelling capacity, cytotoxicity evaluation, and mixed biofilm formation ability were used to characterize the copolymer hydrogels. Hydrogels were evaluated by considering the guidance and regulations of ISO and ASTM standards. The polymers were dense, had stable cross-linking between both monomers, were non-toxic to the Human Embryonic Kidney (HEK) 293 cell line, and reduced bacterial biofilm formation statistically significantly. Furthermore, increasing the amount of MA affected TGF-1 gene expression, where the gene expression was significantly elevated, especially at the highest percentage of MA. Furthermore, the high percentage of MA in the polymer improved the new polymer's thermal properties, film flexibility, and swelling capacity. These novel polymers could be promising materials for improving catheter biomaterial properties and modifying the surfaces of designated devices to reduce microbial infections and growth.

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来源期刊
Biofouling
Biofouling 生物-海洋与淡水生物学
CiteScore
5.00
自引率
7.40%
发文量
57
审稿时长
1.7 months
期刊介绍: Biofouling is an international, peer-reviewed, multi-discliplinary journal which publishes original articles and mini-reviews and provides a forum for publication of pure and applied work on protein, microbial, fungal, plant and animal fouling and its control, as well as studies of all kinds on biofilms and bioadhesion. Papers may be based on studies relating to characterisation, attachment, growth and control on any natural (living) or man-made surface in the freshwater, marine or aerial environments, including fouling, biofilms and bioadhesion in the medical, dental, and industrial context. Specific areas of interest include antifouling technologies and coatings including transmission of invasive species, antimicrobial agents, biological interfaces, biomaterials, microbiologically influenced corrosion, membrane biofouling, food industry biofilms, biofilm based diseases and indwelling biomedical devices as substrata for fouling and biofilm growth, including papers based on clinically-relevant work using models that mimic the realistic environment in which they are intended to be used.
期刊最新文献
Exploring the potential of hydrolytic enzymes combined with antibacterial agents to disrupt pathogenic biofilms and disinfect released cells. Anti-biofilm effect of ferulic acid against Enterobacter hormaechei and Klebsiella pneumoniae: in vitro and in silico investigation. Anti-biofouling marine diterpenoids from Okinawan soft corals. Effects of epigallocatechin gallate on the development of matrix-rich Streptococcus mutans biofilm. Inhibition of Salmonella Typhimurium biofilm and polysaccharide production via eugenol-glucosyltransferase interactions.
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