Junli Zhang, Yanyan Zhao, Haiying Hu, Min Lv, Hongling Zhang
{"title":"以Ni@Pt纳米颗粒作为信号标记的比色纳米酶法快速灵敏地检测血浆中外泌体Aβ42","authors":"Junli Zhang, Yanyan Zhao, Haiying Hu, Min Lv, Hongling Zhang","doi":"10.1007/s00604-024-06862-8","DOIUrl":null,"url":null,"abstract":"<p> A nano-enzyme sandwich assay (SWzyme assay), a colorimetric system based on a biochip and inorganic nano-enzyme for rapid and simple determination of exosomal Aβ42 in plasma is proposed. Anti-CD63 antibody-modified biochips were prepared for plasma exosome capture and synthesized highly catalytic Ni@Pt nanozymes for detecting exosomal Aβ42. The method was able to detect exosomal Aβ42 with a limit of detection (LOD) as low as 4.2×10<sup>4</sup> particles/mL and a linear range from 10<sup>4</sup> to 10<sup>8</sup> particles/mL. By determination of exosomal Aβ42, the SWzyme assay successfully distinguished plasma from Alzheimer’s desease (AD) and healthy mice. The SWzyme assay holds promise to serve as diagnostic tools for the early detection of AD and supporting the development of personalized medicine.</p>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 1","pages":""},"PeriodicalIF":5.3000,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A colorimetric nano-enzyme assay with Ni@Pt nanoparticles as signal labels for rapid and sensitive detection of exosomal Aβ42 in plasma\",\"authors\":\"Junli Zhang, Yanyan Zhao, Haiying Hu, Min Lv, Hongling Zhang\",\"doi\":\"10.1007/s00604-024-06862-8\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p> A nano-enzyme sandwich assay (SWzyme assay), a colorimetric system based on a biochip and inorganic nano-enzyme for rapid and simple determination of exosomal Aβ42 in plasma is proposed. Anti-CD63 antibody-modified biochips were prepared for plasma exosome capture and synthesized highly catalytic Ni@Pt nanozymes for detecting exosomal Aβ42. The method was able to detect exosomal Aβ42 with a limit of detection (LOD) as low as 4.2×10<sup>4</sup> particles/mL and a linear range from 10<sup>4</sup> to 10<sup>8</sup> particles/mL. By determination of exosomal Aβ42, the SWzyme assay successfully distinguished plasma from Alzheimer’s desease (AD) and healthy mice. The SWzyme assay holds promise to serve as diagnostic tools for the early detection of AD and supporting the development of personalized medicine.</p>\",\"PeriodicalId\":705,\"journal\":{\"name\":\"Microchimica Acta\",\"volume\":\"192 1\",\"pages\":\"\"},\"PeriodicalIF\":5.3000,\"publicationDate\":\"2025-01-03\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Microchimica Acta\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s00604-024-06862-8\",\"RegionNum\":2,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microchimica Acta","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1007/s00604-024-06862-8","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
A colorimetric nano-enzyme assay with Ni@Pt nanoparticles as signal labels for rapid and sensitive detection of exosomal Aβ42 in plasma
A nano-enzyme sandwich assay (SWzyme assay), a colorimetric system based on a biochip and inorganic nano-enzyme for rapid and simple determination of exosomal Aβ42 in plasma is proposed. Anti-CD63 antibody-modified biochips were prepared for plasma exosome capture and synthesized highly catalytic Ni@Pt nanozymes for detecting exosomal Aβ42. The method was able to detect exosomal Aβ42 with a limit of detection (LOD) as low as 4.2×104 particles/mL and a linear range from 104 to 108 particles/mL. By determination of exosomal Aβ42, the SWzyme assay successfully distinguished plasma from Alzheimer’s desease (AD) and healthy mice. The SWzyme assay holds promise to serve as diagnostic tools for the early detection of AD and supporting the development of personalized medicine.
期刊介绍：
As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.
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