Kecong Zhou, Huiwen Chen, Jiachen Dong, Zhongchen Song, Mengjun Sun
{"title":"通过生物信息学分析和实验验证鉴定牙周炎中的铁蛋白沉积相关基因","authors":"Kecong Zhou, Huiwen Chen, Jiachen Dong, Zhongchen Song, Mengjun Sun","doi":"10.1016/j.jormas.2025.102291","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Periodontitis is a multifactorial chronic inflammatory disease of periodontal tissues. Ferroptosis is a form of regulated cell death, which is characterized by iron-dependent lipid peroxidation and involved in various inflammatory diseases. This study aims to identify ferroptosis-related genes associated with periodontitis and further validate their relevance through in vitro experiments.</p><p><strong>Methods: </strong>Iron accumulation and localization were detected using Prussian blue staining. Differentially expressed genes in periodontitis were identified from GSE16134 and GSE10334, and intersected with ferroptosis genes to obtain differentially expressed ferroptosis genes (FerDEGs). Functional enrichment analyses of FerDEGs were performed by GO and KEGG. Hub genes were screened through PPI network analysis. The expression of these hub genes in gingival tissues and in lipopolysaccharide (LPS)-stimulated human gingival fibroblasts (HGFs) with/without ferrostatin-1 (Fer-1) detected by qRT-PCR and Western Blot.</p><p><strong>Results: </strong>Ferroptosis was observed in gingival tissues affected by periodontitis. A total of 24 FerDEGs involved in periodontitis were identified. GO analysis and KEGG analysis highlighted the intrinsic apoptotic signaling pathway and ferroptosis as the top enriched pathways. PPI network analysis identified five hub genes. The mRNA expression levels of hub genes were significantly higher in inflammatory gingival tissues and HGFs stimulated with LPS (P < 0.05). The upregulated expression of PTGS2 and IL6 in HGFs were reversed by Fer-1 (P < 0.05).</p><p><strong>Conclusion: </strong>This study highlights five ferroptosis-related genes as potential targets for future research into the pathogenesis of periodontitis.</p>","PeriodicalId":56038,"journal":{"name":"Journal of Stomatology Oral and Maxillofacial Surgery","volume":" ","pages":"102291"},"PeriodicalIF":2.2000,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Identification of ferroptosis-related genes in periodontitis through bioinformatics analysis and experimental validation.\",\"authors\":\"Kecong Zhou, Huiwen Chen, Jiachen Dong, Zhongchen Song, Mengjun Sun\",\"doi\":\"10.1016/j.jormas.2025.102291\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Periodontitis is a multifactorial chronic inflammatory disease of periodontal tissues. Ferroptosis is a form of regulated cell death, which is characterized by iron-dependent lipid peroxidation and involved in various inflammatory diseases. This study aims to identify ferroptosis-related genes associated with periodontitis and further validate their relevance through in vitro experiments.</p><p><strong>Methods: </strong>Iron accumulation and localization were detected using Prussian blue staining. Differentially expressed genes in periodontitis were identified from GSE16134 and GSE10334, and intersected with ferroptosis genes to obtain differentially expressed ferroptosis genes (FerDEGs). Functional enrichment analyses of FerDEGs were performed by GO and KEGG. Hub genes were screened through PPI network analysis. The expression of these hub genes in gingival tissues and in lipopolysaccharide (LPS)-stimulated human gingival fibroblasts (HGFs) with/without ferrostatin-1 (Fer-1) detected by qRT-PCR and Western Blot.</p><p><strong>Results: </strong>Ferroptosis was observed in gingival tissues affected by periodontitis. A total of 24 FerDEGs involved in periodontitis were identified. GO analysis and KEGG analysis highlighted the intrinsic apoptotic signaling pathway and ferroptosis as the top enriched pathways. PPI network analysis identified five hub genes. The mRNA expression levels of hub genes were significantly higher in inflammatory gingival tissues and HGFs stimulated with LPS (P < 0.05). The upregulated expression of PTGS2 and IL6 in HGFs were reversed by Fer-1 (P < 0.05).</p><p><strong>Conclusion: </strong>This study highlights five ferroptosis-related genes as potential targets for future research into the pathogenesis of periodontitis.</p>\",\"PeriodicalId\":56038,\"journal\":{\"name\":\"Journal of Stomatology Oral and Maxillofacial Surgery\",\"volume\":\" \",\"pages\":\"102291\"},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2025-02-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Stomatology Oral and Maxillofacial Surgery\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1016/j.jormas.2025.102291\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"Dentistry\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Stomatology Oral and Maxillofacial Surgery","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1016/j.jormas.2025.102291","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Dentistry","Score":null,"Total":0}
Identification of ferroptosis-related genes in periodontitis through bioinformatics analysis and experimental validation.
Background: Periodontitis is a multifactorial chronic inflammatory disease of periodontal tissues. Ferroptosis is a form of regulated cell death, which is characterized by iron-dependent lipid peroxidation and involved in various inflammatory diseases. This study aims to identify ferroptosis-related genes associated with periodontitis and further validate their relevance through in vitro experiments.
Methods: Iron accumulation and localization were detected using Prussian blue staining. Differentially expressed genes in periodontitis were identified from GSE16134 and GSE10334, and intersected with ferroptosis genes to obtain differentially expressed ferroptosis genes (FerDEGs). Functional enrichment analyses of FerDEGs were performed by GO and KEGG. Hub genes were screened through PPI network analysis. The expression of these hub genes in gingival tissues and in lipopolysaccharide (LPS)-stimulated human gingival fibroblasts (HGFs) with/without ferrostatin-1 (Fer-1) detected by qRT-PCR and Western Blot.
Results: Ferroptosis was observed in gingival tissues affected by periodontitis. A total of 24 FerDEGs involved in periodontitis were identified. GO analysis and KEGG analysis highlighted the intrinsic apoptotic signaling pathway and ferroptosis as the top enriched pathways. PPI network analysis identified five hub genes. The mRNA expression levels of hub genes were significantly higher in inflammatory gingival tissues and HGFs stimulated with LPS (P < 0.05). The upregulated expression of PTGS2 and IL6 in HGFs were reversed by Fer-1 (P < 0.05).
Conclusion: This study highlights five ferroptosis-related genes as potential targets for future research into the pathogenesis of periodontitis.
期刊介绍：
J Stomatol Oral Maxillofac Surg publishes research papers and techniques - (guest) editorials, original articles, reviews, technical notes, case reports, images, letters to the editor, guidelines - dedicated to enhancing surgical expertise in all fields relevant to oral and maxillofacial surgery: from plastic and reconstructive surgery of the face, oral surgery and medicine, … to dentofacial and maxillofacial orthopedics.
Original articles include clinical or laboratory investigations and clinical or equipment reports. Reviews include narrative reviews, systematic reviews and meta-analyses.
All manuscripts submitted to the journal are subjected to peer review by international experts, and must:
Be written in excellent English, clear and easy to understand, precise and concise;
Bring new, interesting, valid information - and improve clinical care or guide future research;
Be solely the work of the author(s) stated;
Not have been previously published elsewhere and not be under consideration by another journal;
Be in accordance with the journal''s Guide for Authors'' instructions: manuscripts that fail to comply with these rules may be returned to the authors without being reviewed.
Under no circumstances does the journal guarantee publication before the editorial board makes its final decision.
The journal is indexed in the main international databases and is accessible worldwide through the ScienceDirect and ClinicalKey Platforms.
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