brusatol抑制真核蛋白合成的机制

Walker Willingham Jr. , Edward A. Stafford , Steven H. Reynolds , Stephen G. Chaney , Kuo-Hsiung Lee , Masayoshi Okano , Iris H. Hall
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引用次数: 34

摘要

研究了brusatol抑制兔网织红细胞蛋白合成的机制。当添加到网状细胞裂解物时,brusatol在30°C下仅在滞后2-4分钟后抑制内源性蛋白质合成。在此期间,80s核糖体积累。Brusatol在抑制裂解物中的内源性蛋白质合成和径流核糖体中的聚(U)定向多苯丙氨酸合成方面同样有效。在分级网织细胞系统中,brusatol不抑制三元、40s和80s起始复合物的形成,但抑制嘌呤霉素与含有[35S]Met-tRNAf的起始复合物的反应。这些数据表明,brusatol抑制蛋白质合成的肽基转移酶延伸反应,但只有在完成一轮蛋白质合成后才能起作用。因此,brusatol在兔网织细胞系统中的作用机制与之前报道的brusatol在酵母系统中的作用非常相似。
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Mechanism of eukaryotic protein synthesis inhibition by brusatol

The mechanism by which brusatol inhibits protein synthesis in rabbit reticulocytes has been investigated. When added to reticulocyte lysates, brusatol inhibits endogenous protein synthesis only after a lag of 2–4 min at 30°C. During this period 80 S ribosomes accumulate. Brusatol is equally effective in inhibiting endogenous protein synthesis in lysates and poly(U)-directed polyphenylalanine synthesis with runoff ribosomes. In fractionated reticulocyte systems, brusatol does not inhibit formation of the ternary, 40 S, and 80 S initiation complexes, but does inhibit the reaction of puromycin with initiation complexes containing [35S]Met-tRNAf. These data suggest that brusatol inhibits the peptidyl transferase elongation reaction of protein synthesis, but can do so only after one round of protein synthesis has been completed. Thus, the mechanism of action of brusatol in the rabbit reticulocyte system is very similar to the effects previously reported for bruceantin in a yeast system.

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