己酮茶碱和生长抑素对人肺巨噬细胞产生肿瘤坏死因子的影响。

Circulatory shock Pub Date : 1994-06-01
J L Balibrea, J Arias-Díaz, C García, E Vara
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引用次数: 0

摘要

在脓毒症期间产生有害影响时,细胞因子似乎主要以旁分泌方式起作用。因此,肺巨噬细胞的局部TNF α释放可能在成人呼吸窘迫综合征(ARDS)的发病机制中起核心作用。相比之下,penttoxiphylline (PTXF)可以减轻脓毒症动物模型中的肺损伤,而生长抑素(SS-14)已被证明可以下调单核细胞中TNF α受体的表达,表明该激素具有免疫调节作用。本实验旨在研究PTXF和SS-14对脂多糖(LPS)诱导的人肺巨噬细胞释放TNF α的影响。巨噬细胞来自多器官供体肺。近期有吸烟史、机械通气超过72小时或任何放射性肺浸润者均未纳入本研究。培养1小时后,LPS刺激TNF α释放呈剂量依赖性(2.5和10微克/毫升LPS分别为2.34 +/- 0.20和11.32 +/- 1.38 pg/微克蛋白,P < 0.01)。100微克/ml PTXF (0.24 +/- 0.07 vs. 2.43 +/- 0.20, P < 0.01, 1.30 +/- 0.08 vs. 11.32 +/- 1.38, P < 0.01, pg/微克蛋白,2.5和10微克/ml LPS)和0.4 ng/ml SS-14 (0.26 +/- 0.07 vs. 2.43 +/- 0.20, P < 0.01, 0.60 +/- 0.19 vs. 11.32 +/- 1.38, P < 0.01, pg/微克蛋白,2.5和10微克/ml LPS)显著抑制了这种反应。(摘要删节250字)
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Effect of pentoxifylline and somatostatin on tumour necrosis factor production by human pulmonary macrophages.

Cytokines seem to act predominantly in a paracrine manner when producing their deleterious effects during sepsis. Therefore, local TNF alpha release by pulmonary macrophages would have a central role in the pathogenesis of the adult respiratory distress syndrome (ARDS). By contrast, pentoxiphylline (PTXF) can reduce lung damage in septic animal models, and somatostatin (SS-14) has been shown to down-regulate TNF alpha-receptor expression in monocytes, suggesting an immunomodulatory action for this hormone. The aim of this work was to study the effect of PTXF and SS-14 on lipopolysaccharide (LPS)-induced TNF alpha release by human pulmonary macrophages. Macrophages were obtained from multiple organ donor lungs. Donors with either a recent history of tobacco smoking, more than 72 hr of mechanical ventilation, or any radiological pulmonary infiltrate were not included in this study. After 1 hr of culture, LPS stimulated TNF alpha release in a dose-dependent manner (2.34 +/- 0.20 and 11.32 +/- 1.38 pg/microgram protein, P < 0.01, in response to 2.5 and 10 micrograms/ml LPS, respectively). This response was significantly inhibited by both PTXF, 100 micrograms/ml (0.24 +/- 0.07 vs. 2.43 +/- 0.20, P < 0.01, and 1.30 +/- 0.08 vs. 11.32 +/- 1.38, P < 0.01, pg/micrograms protein, 2.5 and 10 micrograms/ml LPS, respectively) and SS-14, 0.4 ng/ml (0.26 +/- 0.07 vs. 2.43 +/- 0.20, P < 0.01, and 0.60 +/- 0.19 vs. 11.32 +/- 1.38, P < 0.01, pg/micrograms protein, 2.5 and 10 micrograms/ml LPS, respectively).(ABSTRACT TRUNCATED AT 250 WORDS)

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