高渗盐水增强细胞免疫功能。

Circulatory shock Pub Date : 1994-04-01
W G Junger, F C Liu, W H Loomis, D B Hoyt
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引用次数: 0

摘要

高渗盐水(HTS)复苏改善创伤后的预后。我们研究了HTS对免疫功能的影响。细胞外Na+浓度增加25 mM时,人和兔外周血单个核细胞(PBMC)的体外t细胞增殖能力增加一倍。进一步增加的高渗性(人细胞超过40 mM,兔细胞超过80 mM)导致增殖的进行性抑制。人和家兔单核细胞功能(肿瘤坏死因子生成)在30 mM高渗时增强300%,表明hts增强的单核细胞辅助细胞功能可能导致t细胞增殖增加。KCl替代HTS也能增强t细胞的增殖,提示其参与渗透效应。HTS(高达30 mM)增加了未受刺激的人PBMC的Ca2+i。兔注射HTS可提高细胞介导的免疫功能(延迟型超敏反应)。我们的研究结果表明,血浆渗透压升高可能上调细胞免疫功能。因此,创伤患者的HTS复苏可能逆转创伤后免疫抑制并降低败血症的风险。
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Hypertonic saline enhances cellular immune function.

Hypertonic saline (HTS) resuscitation improves outcome after trauma. We studied the effect of HTS on immune function. In vitro T-cell proliferation of human and rabbit peripheral blood mononuclear cells (PBMC) was doubled at 25 mM increased extracellular Na+ concentrations. Further increased hypertonicity (more than 40 mM with human cells, and 80 mM with rabbit cells) caused progressive suppression of proliferation. Human and rabbit monocyte functions (tumor necrosis factor production) were augmented by 300% at 30 mM hypertonicity, indicating that HTS-enhanced accessory cell function of monocytes may cause increased T-cell proliferation. Substitution of HTS with KCl also enhanced T-cell proliferation, suggesting an involvement of osmotic effects. HTS (up to 30 mM) increased Ca2+i of nonstimulated human PBMC. HTS injection in rabbits increased cell-mediated immune function (delayed-type hypersensitivity reaction). Our findings suggest that increased plasma osmolality may up-regulate cellular immune function. HTS resuscitation of trauma patients may thus reverse posttraumatic immunosuppression and reduce the risk of sepsis.

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