{"title":"[辅助或无辅助病毒的HSV-1载体对基因转入大鼠脑的免疫反应的比较]。","authors":"Wei Zhang, A Geller","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>This study was conducted to compare the immune response to gene transfer with helper virus or helper virus-free vectors.</p><p><strong>Methods: </strong>Two vector stocks were delivered by stereotactic injection into the striatum and then the immune response markers including TCR, CD4, CD8, MHC-I and MHC-II were examined.</p><p><strong>Results: </strong>The data showed that the immune response to either vector stock reached maximal levels by 4 to 7 days after gene transfer. However, the maximal levels of all markers to the helper virus-free stock were 3 to 10 folds lower than the markers to the helper virus stock. The immune response to the helper virus-free stock declined rapidly to be close to the levels of PBS control.</p><p><strong>Conclusion: </strong>The immune response to the helper virus-free stock is significantly less than the response to the vector stock that contains helper virus.</p>","PeriodicalId":13173,"journal":{"name":"Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao","volume":"33 2","pages":"175-8"},"PeriodicalIF":0.0000,"publicationDate":"2002-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[Comparison of the immune response to gene transfer into the rat brain with helper or helper virus-free HSV-1 vectors].\",\"authors\":\"Wei Zhang, A Geller\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>This study was conducted to compare the immune response to gene transfer with helper virus or helper virus-free vectors.</p><p><strong>Methods: </strong>Two vector stocks were delivered by stereotactic injection into the striatum and then the immune response markers including TCR, CD4, CD8, MHC-I and MHC-II were examined.</p><p><strong>Results: </strong>The data showed that the immune response to either vector stock reached maximal levels by 4 to 7 days after gene transfer. However, the maximal levels of all markers to the helper virus-free stock were 3 to 10 folds lower than the markers to the helper virus stock. The immune response to the helper virus-free stock declined rapidly to be close to the levels of PBS control.</p><p><strong>Conclusion: </strong>The immune response to the helper virus-free stock is significantly less than the response to the vector stock that contains helper virus.</p>\",\"PeriodicalId\":13173,\"journal\":{\"name\":\"Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao\",\"volume\":\"33 2\",\"pages\":\"175-8\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2002-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
[Comparison of the immune response to gene transfer into the rat brain with helper or helper virus-free HSV-1 vectors].
Objective: This study was conducted to compare the immune response to gene transfer with helper virus or helper virus-free vectors.
Methods: Two vector stocks were delivered by stereotactic injection into the striatum and then the immune response markers including TCR, CD4, CD8, MHC-I and MHC-II were examined.
Results: The data showed that the immune response to either vector stock reached maximal levels by 4 to 7 days after gene transfer. However, the maximal levels of all markers to the helper virus-free stock were 3 to 10 folds lower than the markers to the helper virus stock. The immune response to the helper virus-free stock declined rapidly to be close to the levels of PBS control.
Conclusion: The immune response to the helper virus-free stock is significantly less than the response to the vector stock that contains helper virus.
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