[Nerve tissue morphological study of tutin microinjection into pontine NPBM in two hours].

Objective: To observe whether Tutin microinjection into the pontine NPBM respiratory area of rabbit will cause morphological damage to that area two hours later.

Methods: At two hours after the microinjection of Tutin into NPBM, the experimental effects on respiration came to be remarkable and the physiological condition was well, the rabbit was subjected to morphological sampling then. The sample was cut into slices for LM (Nissl dyeing) and transmission EM observation and photography.

Results: Under the LM and EM examination, no remarkable morphological damage done by Tutin microinjection into the pontine NPBM was observed. By comparing the Tutin-injected side with the other side of NPBM where equal normal saline microinjection was given, no apparent morphological difference could be found.

Conclusion: In our experiment condition, there was no morphological damage caused by Tutin microinjection into pontine NPBM respiratory area of the rabbit.