两种种植材料对三种龈下优势菌生长的影响。

Ning Gao, Xiaorong Xiao, Feng Chai, Suqin Xian, Zhu Zhu, Yurong Liu
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引用次数: 0

摘要

目的:市售纯钛及钛合金作为种植体具有良好的临床效果。尽管成功率很高,但有些植入物确实会失败。与那些关于种植体骨整合的研究相比,有关其失败的信息很少。种植体材料与微生物的关系有待进一步研究。本研究旨在研究两种常用种植材料钛(TA2)和Ti-6AI-4V合金(TC4)对三种龈下优势菌血链球菌(s . s)、牙龈卟啉菌(P. g)、核梭菌(F. n)及其混合物生长行为的影响。方法:在厌氧条件下,S.s、P.g、F.n菌悬液及其混合物分别与两种植入材料一起培养,以相同菌悬液为对照。2、7、14 d后,采用克隆形成单位(CFU)法测定细菌生长量。用pH- meter测定菌悬液的pH值。结果:TA2组与TC4组细菌生长量及pH值差异无统计学意义(P > 0.05)。细菌混合物中单个细菌的比例和培养悬浮液的pH值也没有统计学上的显著变化。结论:在本研究条件下,两种种植材料对龈下细菌的生长及其培养环境的pH值均无明显影响。
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[The influence of two implant materials on the growth of three subgingival predominant bacteria].

Objective: Commercially pure titanium and titanium alloy as dental implants have show impressive clinical results. Despite the high success rates, some implants do fail. Compared to those studies on osseointegration of implants, the information pertaining to their failure is little. Further studies on the relationship between implant material and micro-organism are needed. The purpose of this investigation is to study the effect of two commonly used implant materials Titanium (TA2) and Ti-6AI-4V alloy (TC4) on the growth behaviour of three subgingival predominant bacteria Streptococcus sanguis (S. s), Porphyromanus gingivalis (P. g), Fusobacterium nucleatum (F. n) and their mixture.

Methods: Under anaerobic condition, bacterial suspensions of S.s, P.g, F.n and their mixture were incubated together with the two implant materials respectively, setting the same bacterial suspensions as controls. After 2, 7 and 14 days, the bacterial growth amount was assayed by means of clone forming unit (CFU) method. The pH value of the bacterial suspension was determined by pH-Meter.

Results: The results showed that there was no difference in amount of bacterial growth or pH value between TA2 group and TC4 group (P > 0.05). There was also no statistically significant change as to the proportion of individual bacteria in bacterial mixture or the pH value of culture suspension.

Conclusion: Under the condition of this investigation, the two studies implant materials have no examined influences on the growth of the subgingival bacteria and the pH value of their culture environment.

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