{"title":"缺氧诱导因子1 α在实验性脊髓损伤中的序列变化及其意义。","authors":"Yan Ju, Min He, Boyong Mao, Lida Gao","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To investigate the sequential changes of hypoxia-inducible factor 1 alpha (HIF-1 alpha) in experimental spinal cord(SCI) injury in rats and analyze its potential effects on SCI.</p><p><strong>Methods: </strong>Static compression model of SCI was employed in this study. Expressions of HIF-1 alpha were measured with immunohistochemical staining. Flow cytometry was used to determine the apoptotic ratio and bcl-2 expressions.</p><p><strong>Results: </strong>HIF-1 alpha began to increase one day after injury, and reached peak at 3-7 days. Two weeks later, it declined significantly. The sequential changes of HIF-1 alpha coincided well with the alterations of apoptotic ratio and bcl-2 content.</p><p><strong>Conclusion: </strong>HIF-1 alpha may participate in the secondary ischemic and hypoxic procedures after spinal cord injury and mediate the traumatic apoptosis. Further understanding of HIF-1 alpha may provide new therapeutic regimens for SCI.</p>","PeriodicalId":13173,"journal":{"name":"Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao","volume":"33 1","pages":"43-5"},"PeriodicalIF":0.0000,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[Sequential changes of hypoxia-inducible factor 1 alpha in experimental spinal cord injury and its significance].\",\"authors\":\"Yan Ju, Min He, Boyong Mao, Lida Gao\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>To investigate the sequential changes of hypoxia-inducible factor 1 alpha (HIF-1 alpha) in experimental spinal cord(SCI) injury in rats and analyze its potential effects on SCI.</p><p><strong>Methods: </strong>Static compression model of SCI was employed in this study. Expressions of HIF-1 alpha were measured with immunohistochemical staining. Flow cytometry was used to determine the apoptotic ratio and bcl-2 expressions.</p><p><strong>Results: </strong>HIF-1 alpha began to increase one day after injury, and reached peak at 3-7 days. Two weeks later, it declined significantly. The sequential changes of HIF-1 alpha coincided well with the alterations of apoptotic ratio and bcl-2 content.</p><p><strong>Conclusion: </strong>HIF-1 alpha may participate in the secondary ischemic and hypoxic procedures after spinal cord injury and mediate the traumatic apoptosis. Further understanding of HIF-1 alpha may provide new therapeutic regimens for SCI.</p>\",\"PeriodicalId\":13173,\"journal\":{\"name\":\"Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao\",\"volume\":\"33 1\",\"pages\":\"43-5\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2002-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
[Sequential changes of hypoxia-inducible factor 1 alpha in experimental spinal cord injury and its significance].
Objective: To investigate the sequential changes of hypoxia-inducible factor 1 alpha (HIF-1 alpha) in experimental spinal cord(SCI) injury in rats and analyze its potential effects on SCI.
Methods: Static compression model of SCI was employed in this study. Expressions of HIF-1 alpha were measured with immunohistochemical staining. Flow cytometry was used to determine the apoptotic ratio and bcl-2 expressions.
Results: HIF-1 alpha began to increase one day after injury, and reached peak at 3-7 days. Two weeks later, it declined significantly. The sequential changes of HIF-1 alpha coincided well with the alterations of apoptotic ratio and bcl-2 content.
Conclusion: HIF-1 alpha may participate in the secondary ischemic and hypoxic procedures after spinal cord injury and mediate the traumatic apoptosis. Further understanding of HIF-1 alpha may provide new therapeutic regimens for SCI.
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